Immune Cell Responses in Gastric Carcinoma

Ohtani, Haruo

doi:10.5772/17472

Cited by 1 publication

(1 citation statement)

References 17 publications

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“…Carcinoma with lym-phoid stroma, also known as lymphoepithelioma-like carcinoma is a rare histopathological variant of carcinoma in various organs and is characterized by the formation of a TIL-rich stroma, whose prognosis is usually favorable [5,9,15,22]. We have histopathologically analyzed this variant of carcinoma from the perspective of host immune responses to carcinoma cells [11][12][13][14]17]. In 2009, we used the term tertiary lymphoid tissue for the lymphoid stroma of gastric carcinoma due to its similarity to secondary lymphoid organs [11,Fig.…”

Section: Introductionmentioning

confidence: 99%

C-C Chemokine 21-Expressing T-cell Zone Fibroblastic Reticular Cells, Abundant in Lymph Nodes, Are Absent in Cancer Lymphoid Stroma

Ohtani,

Matsuo,

Kitahata

et al. 2024

Acta Histochem. Cytochem

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Cancer tissue generally possesses an immunosuppressive microenvironment. However, some cancers are associated with lymphoid stroma (i.e., a widely developed tertiary lymphoid structure). The T-cell zone (paracortex) of secondary lymphoid organs, particularly lymph nodes, is characterized by an abundance of T-cell zone fibroblastic reticular cells (TCZ-FRCs) that express C-C motif chemokine ligand 21 (CCL21) and smooth muscle actin (SMA). We analyzed the presence of TCZ-FRCs in 30 cases of carcinomas with lymphoid stroma of the breast, stomach, colon, tongue, and skin. Immunohistochemistry corroborated the abundance of CCL21 + SMA + TCZ-FRCs in the normal lymph nodes. In sharp contrast, all 30 carcinomas with lymphoid stroma displayed no CCL21 + SMA + TCZ-FRCs despite the affluence of T cells. Real-time reverse transcription polymerase chain reaction confirmed a marked decrease in the messenger ribonucleic acid expression of CCL21 and its receptor C-C motif chemokine receptor 7 in cancer lymphoid stroma compared to that in lymph nodes. Next, we analyzed the T cell phenotypes. The cancer lymphoid stroma demonstrated an abundance of CD3 + CD62L − memory-type T cells, in contrast to the presence of CD3 + CD62L + naïve- and central memory T cells in the T cell zone of lymphoid tissues. Our data demonstrated the following: 1) Cancer lymphoid stroma lacked TCZ-FRCs with abundance of more activated T cells than in lymph nodes and 2) these were common phenomena in cancer lymphoid stroma irrespective of the histological types and organs involved.

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