Immune and haematological parameters of Blackbelly ewes infected with gastrointestinal nematodes

González-Gardúño, Roberto; Arellano, María Eugenia López; Felipe, Magnolia María Conde de; Gives, Pedro Mendoza de; Marcelino, Liliana Aguilar; Díaz, Gabriela Jaso

doi:10.17533/udea.rccp.v30n3a05

Cited by 7 publications

(4 citation statements)

References 19 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this case, selecting individuals among different breeds may be favorable even for Crioulas pregnant with twins; besides, a combination of genetic selection and nutritional supplementation in Crioula herd would be effective management against parasitism. Contrary to what Ribeiro et al (36) reported, we did not observe a constant regression on TPP but a marked decline after parturition (from 8.6 to 5.8 g/dl), observed in BlackBelly (32) and Ile de France sheep as well (8,9). Values of TPP may reach around 5 g/dl in susceptible ewes (12) during the postpartum.…”

Section: Discussioncontrasting

confidence: 99%

“…The slight increase observed in PCV during gestation is attributed to the greater need of oxygen transport in pregnant ewes, because of their higher metabolic rate compared to nonpregnant ewes (44). However, the EPG peak at the parturition leads to a reduction in the PCV during lactation, in response to the susceptibility of the females to the infection especially when the parasite has the behavior of feeding from the host blood, such as H. contortus (32). The observed decrease was considered critical only in the group of susceptible ewes, reaching 25% probably because most ewes from this group were pregnant with twins.…”

Section: Discussionmentioning

confidence: 98%

“…Peripartum is characterized by physiological depression of immunity and increase of EPG values at different intensities and moments. Peaks may occur early in lactation (31), or in the course of it (32), depending on the breed, but differences can be noticed even in ewes belonging to the same breed. Research carried out with Santa Inês hair sheep showed this variation.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Peripartum Phenomenon in Crioula Lanada Sheep Susceptible and Resistant to Gastrointestinal Nematodes

et al. 2020

View full text Add to dashboard Cite

This study aimed at evaluating parasitological and blood variables in native breed Crioula Lanada sheep belonging to the same herd, to identify and compare susceptible or resistant individuals to gastrointestinal nematodes during gestation and lactation phases. For this purpose, 18 Crioula sheep were used within 2 years of evaluation, in which blood and feces samples and weight of the animals were taken from their 4th month of gestation until the weaning of their lambs, in the 4th month postpartum. Feces samples were used for counting eggs per gram of feces (EPG) and, thus, to identify 12 resistant ewes (EPG < 1,000) and 6 susceptible (EPG > 1,000) to gastrointestinal nematodes. The identification of infective larvae was also performed. Blood was used for analysis of packed cell volume (PCV), eosinophil count, total plasma protein, and immunoglobulin G level against Haemonchus contortus infective larvae. The Kruskal-Wallis non-parametric comparison test was used to evaluate the differences between days of parturition and linear mixed-effects model using package lme4 in R to evaluate the groups. The main parasite species observed in the feces samples were Haemonchus, Trichostrongylus, Oesophagostomum, Ostertagia, and Cooperia in similar proportions in both groups. Susceptible ewes presented peaks of EPG at the beginning of lactation and lower PCV values throughout the study. No difference between groups was observed concerning other blood variables or body weight, but some changes were observed along with the gestation and lactation phases within each group. The physiological response of sheep to nematode infection is a useful tool to identify the most susceptible individuals within the same breed and herd and to select the most genetically resistant individuals.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Peripartum Phenomenon in Crioula Lanada Sheep Susceptible and Resistant to Gastrointestinal Nematodes

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The immunoglobulin levels (IgA, IgG, and IgM) were determined by means of an indirect enzyme-linked immunosorbent assay (iELISA) using crude antigens (CAg) of H. contortus and Trichostrongylus colubriformis according to the procedure described by Gonz alez Garduño et al (2017). The optical density (OD) of each serum was obtained by subtracting from each well the values of the wells without serum, which represented the non-specific binding of the conjugate and the activity of the Igs were expressed as a percentage to a positive standard serum (RPS) to calibrate the values among the plates.…”

Section: Animal Managementmentioning

confidence: 99%

High energy levels in the diet reduce the parasitic effect of Haemonchus contortus in Pelibuey sheep

López-Leyva

González-Gardúño

Huerta-Bravo

et al. 2020

Heliyon

View full text Add to dashboard Cite

Nutritional aspects modulate the parasitological and immune response in infected sheep. The aim of the present study was to evaluate the parasitological and humoral immune response of Pelibuey sheep experimentally infected with Haemonchus contortus that were fed diets with two different energy (n ¼ 12) and protein (n ¼ 12) levels. Twenty-four Pelibuey lambs infected with H. contortus were evaluated over 12 weeks. An additional six animals were considered as a control group. Fecal egg count (FEC) was determined, in addition to packed cell volume (PCV), total plasma protein (TPP), and immunoglobulin levels (IgA, IgG, and IgM) by indirect enzymelinked immunosorbent assay (iELISA). Data were analyzed by repeated measures over time. The lambs that received a high-energy (HighE) diet had the lowest FEC (P < 0.01) regardless of whether they received a high (HighP) or low (LowP) level of protein. The effect of energy level was also observed over time: FEC values decreased and PCV and TPP values increased. Higher immunoglobulin levels were obtained for females (P < 0.05) than males yet, overall, the energy and protein levels of the diets did not affect the response of the immunoglobulins. Over time, however, an increase in IgG and IgM was observed, whereas the IgA level remained basal.

show abstract

Influence of the physiological stage of Blackbelly sheep on immunological behaviour against gastrointestinal nematodes

González-Gardúño

Gives

Arellano

et al. 2018

Experimental Parasitology

View full text Add to dashboard Cite

Immune and haematological parameters of Blackbelly ewes infected with gastrointestinal nematodes

Cited by 7 publications

References 19 publications

Peripartum Phenomenon in Crioula Lanada Sheep Susceptible and Resistant to Gastrointestinal Nematodes

Peripartum Phenomenon in Crioula Lanada Sheep Susceptible and Resistant to Gastrointestinal Nematodes

High energy levels in the diet reduce the parasitic effect of Haemonchus contortus in Pelibuey sheep

Influence of the physiological stage of Blackbelly sheep on immunological behaviour against gastrointestinal nematodes

Contact Info

Product

Resources

About