Immune and bacterial toxin genes expression in different giant tiger prawn, penaeus monodon post-larvae stages following AHPND-causing strain of vibrio parahaemolyticus challenge

Deris, Zulaikha Mat; Iehata, Shumpei; Ikhwanuddin, Mhd; Sahimi, Mohd Badrul Mohamad Khairul; Do, Thinh Dinh; Sorgeloos, Patrick; Sung, Yeong Yik; Wong, Li Lian

doi:10.1016/j.aqrep.2019.100248

Cited by 21 publications

(19 citation statements)

References 59 publications

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“…Importantly, early developmental stages of animals have been reported for a higher infection risk by pathogenic microorganisms than adult stage 10 , 11 . In shrimp, AHPND causes severe mortality in early stages of shrimp including P. monodon 12 – 14 . Additionally, the infectious hypodermal and hematopoietic necrosis virus (IHHNV) causing runt deformity syndrome (RDS) and EHP can infect shrimp at the early life stage which can further transmit to later shrimp developmental stages, resulting in poor survival rates or growth performance in shrimp aquaculture 15 , 16 .…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages

Angthong

Uengwetwanit

Arayamethakorn

et al. 2021

Sci Rep

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With the rapid growth in the global demand, the shrimp industry needs integrated approaches for sustainable production. A high-quality shrimp larva is one of the crucial key requirements to maximize shrimp production. Survival and growth rates during larval development are often criteria to evaluate larval quality, however many aspects of gene regulation during shrimp larval development have not yet been identified. To further our understanding of biological processes in their early life, transcriptomic analysis of larval developmental stages (nauplius, zoea, mysis, and postlarva) were determined in the black tiger shrimp, Penaeus monodon using next-generation RNA sequencing. Gene clustering and gene enrichment analyses revealed that most of the transcripts were mainly related to metabolic processes, cell and growth development, and immune system. Interestingly, Spätzle and Toll receptors were found in nauplius stage, providing evidence that Toll pathway was a baseline immune system established in early larval stages. Genes encoding pathogen pattern-recognition proteins (LGBP, PL5-2 and c-type lectin), prophenoloxidase system (PPAE2, PPAF2 and serpin), antimicrobial peptides (crustin and antiviral protein), blood clotting system (hemolymph clottable protein) and heat shock protein (HSP70) were expressed as they developed further, suggesting that these immune defense mechanisms were established in later larval stages.

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Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages

Angthong

Uengwetwanit

Arayamethakorn

et al. 2021

Sci Rep

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“…Immune roles of the TLRs during ontogeny should not be underestimated: Many marine invertebrate embryos and larvae live in environments rich in microbial pathogens [132,133]. Pathogens cause mortality of embryos and larvae but also provoke anomalies during development [134,135]. Therefore, these embryos and larvae need immune defenses to ght pathogens [133].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, these embryos and larvae need immune defenses to ght pathogens [133]. Actually, few studies have shown that the Toll pathway is involved in immunity during ontogeny in arthropods, mollusks and amphioxus [18,[135][136][137], and other immune-related genes have also been found to be involved in immunity during mollusk and echinoderm development [136,[138][139][140]. Additionally, in planulozoans it has been shown that TLRs are involved in adult immunity [11,[22][23][24][25][26].…”

Section: Discussionmentioning

confidence: 99%

The evolution of the metazoan Toll receptor family and its expression during protostome development

Orús-Alcalde

Hejnol

2021

Preprint

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Background: Toll-like receptors (TLRs) play a crucial role in immunity and development. They contain leucine-rich repeat domains, one transmembrane domain, and one Toll/IL-1 receptor domain. TLRs have been classified into V-type/scc and P-type/mcc TLRs, based on differences in the leucine-rich repeat domain region. Although TLRs are widespread in animals, detailed phylogenetic studies of this gene family are lacking. Here we aim to uncover TLR evolution by conducting a survey and a phylogenetic analysis in species across Bilateria. To discriminate between their role in development and immunity we furthermore analyzed stage-specific transcriptomes of the ecdysozoans Priapulus caudatus and Hypsibius exemplaris, and the spiralians Crassostrea gigas and Terebratalia transversa.Results: We detected a low number of TLRs in ecdysozoan species, and multiple independent radiations within the Spiralia. V-type/scc and P-type/mcc type-receptors are present in cnidarians, protostomes and deuterostomes, and therefore they emerged early in TLR evolution, followed by a loss in xenacoelomorphs. Our phylogenetic analysis shows that TLRs cluster into three major clades: clade α is present in cnidarians, ecdysozoans, and spiralians; clade β in deuterostomes, ecdysozoans, and spiralians; and clade γ is only found in spiralians. Our stage-specific transcriptome and in situ hybridization analyses show that TLRs are expressed during development in all species analyzed, which indicates a broad role of TLRs during animal development.Conclusions: Our findings suggest that the bilaterian TLRs likely emerged by duplication from a single TLR encoding gene (proto-TLR) present in the last common cnidarian-bilaterian ancestor. This proto-TLR gene duplicated before the split of protostomes and deuterostomes; a second duplication occurred in the lineage to the Trochozoa. While all three clades further radiated in several spiralian lineages, specific TLRs clades have been presumably lost in others. Furthermore, the expression of the majority of these genes during protostome ontogeny suggests their involvement in immunity and development.

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“…The first baseline defense mechanism of shrimp humoral immune response is the clotting system, which is vital for the prevention of haemolymph damage during injury and wound healing and prevents the entry of opportunistic pathogens (Maningas et al 2013). On the other hand, P. monodon cellular immune response involves different types of cells, including haemocytes, which are initiated by a cascade of phenoloxidase (PO) activating processes leading to phagocytosis, encapsulation, coagulation, and melanisation of invading pathogens (Deris et al 2020). In the present study, methanolicextract of P. tetrastromatica incorporated into diets and fed to shrimp, lowered haemolymph clotting time after 10 days of exposure with V. parahaemolyticus bacteria, thus indicating good immune effect in the treated group.…”

Section: The Haematological and Immunostimulatory Activity Of Penaeusmentioning

confidence: 99%

“…Shrimp have innate immunity to combat pathogenic infections, which is a first-line defense mechanism (Deris et al 2020). This system eliminates invading microorganisms by humoral and cellular immune responses (S€ oderh€ all and Cerenius 1998).…”

Section: Introductionmentioning

confidence: 99%

Effects of seaweeds extract on growth, survival, antibacterial activities, and immune responses of Penaeus monodon against Vibrio parahaemolyticus

Aftabuddin

Siddique

Habib

et al. 2021

Italian Journal of Animal Science

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Seaweeds can play an essential role in controlling antibiotic resistance for bacterial diseases. Three extracts of two brown seaweeds Padina tetrastromatica and Sargassum ilicifolium were investigated as growth promoters and to provide immune protection against Vibrio parahaemolyticus. Extracts of the two seaweeds were administered at 2.5 and 5 g kg À1 with food for 45 days to Penaeus monodon juveniles. The maximum weight gain (6.28 and 7.08 g), specific growth rate (3.39 and 3.32%), and survival rate (78.45 and 82.45%) of the juvenile tiger shrimp were obtained at a dose of 5 g kg À1 with P. tetrastromatica extracted by methanol and ethanol, respectively; which was significantly higher than the other solvents and control group (p < .01). On the other hand, methanol extract of both seaweeds had maximal antibacterial activity against V. parahaemolyticus with zones of inhibition ranging between 20.25 ± 1.00 and 22.00 ± 0.50 mm. The minimal time required for haemolymph clotting was with methanol extracted of P. tetrastromatica at 5 g kg À1 , which was significantly lower than the other groups (p < .05). After challenging with V. parahaemolyticus, the total haemocyte count (THC) in P. monodon treated with methanol-extract of P. tetrastromatica diet at 5 g kg À1 was higher (5.42 Â 10 5 cells mL À1 ) but not significantly different than the other diets (p > .05). However, the administration of 5 g kg À1 methanol and ethanol extract of P. tetrastromatica improved growth performance and the immune capacity of P. monodon by increasing their phenoloxidase activity, superoxide anion concentration, and resistance against V. parahaemolyticus. HIGHLIGHTSThe maximum growth performance and survival of juvenile tiger shrimps obtained with Padina tetrastromatica extract at a dose of 5 g kg À1 . The methanol extract of P. tetrastromatica and Sargassum ilicifolium had maximal antibacterial activity against V. parahaemolyticus. The administration of 5 g kg À1 methanol and ethanol extract of P. tetrastromatica improved growth performance and the immune capacity of P. monodon.

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Immune and bacterial toxin genes expression in different giant tiger prawn, penaeus monodon post-larvae stages following AHPND-causing strain of vibrio parahaemolyticus challenge

Cited by 21 publications

References 59 publications

Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages

Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages

The evolution of the metazoan Toll receptor family and its expression during protostome development

Effects of seaweeds extract on growth, survival, antibacterial activities, and immune responses of Penaeus monodon against Vibrio parahaemolyticus

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