Immobilized Thrombin Receptor Agonist Peptide Accelerates Wound Healing in Mice

Strukova, S. M.; Dugina, T. N.; Chistov, I.V.; Lange, M; Марквичева, Елена; Купцова, С.В.; Зубов, В. П.; Glusa, Erika

doi:10.1177/107602960100700414

Cited by 40 publications

(30 citation statements)

References 24 publications

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“…The platelets were activated with thrombin, a naturally occurring platelet activator that promotes wound healing. This fact induces a decreased wound size by 60%, increased the fibroblast to macrophage ratio and increased proliferating fibroblast 150% 12 . This activation of platelets also causes release of VEGF a mediator of angiogenesis that stimulates endothelial cell proliferation 13 .…”

Section: Resultsmentioning

confidence: 99%

Effects of platelet-rich plasma gel on skin healing in surgical wound in horses

DeRossi¹,

Coelho²,

Mello³

et al. 2009

Acta Cir. Bras.

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Purpose: To establish a low-cost method to prepare platelet-rich plasma (PRP) and evaluates the potential of platelet derived factors to enhance wound healing in the surgical wounds in equine. Methods: To obtain a PRP gel, calcium gluconate and autologous thrombin were added to platelet-rich plasma. For the tests six saddle horses were used and two surgical incisions were made in each animal. Wounds were treated with PRP gel or untreated. Sequential wound biopsies collected at Treatment 1: at days 5 and 30 and Treatment 2: at days 15 and 45 post wounding permitted comparison of differentiation markers and wound repair. Results: The optimal platelets enrichment over 4.0 time's baseline values was obtained using 300 g for 10 min on the first centrifugation and 640 g for 10 min on the second centrifugation. Conclusion: Wounds treated with PRP gel exhibit more rapid epithelial differentiation and enhanced organization of dermal collagen compared to controls in equine Key words: Platelet-Rich Plasma. Wound Healing. Skin. Horses. RESUMOObjetivo: Estabelecer um método econômico na preparação de plasma rico em plaquetas (PRP) e avaliar se os fatores derivados destas plaquetas aceleram a cicatrização de feridas cirúrgicas em cavalos. Métodos: Gluconato de cálcio e trombina autógena foram adicionados ao PRP para a obtenção do gel de PRP. Foram usados seis cavalos de sela, cada um dos quais sofreu duas incisões cirúrgicas. Uma destas incisões foi tratada com gel de PRP e a outra suturada de maneira tradicional (controle). A biópsia das feridas foi coletada de maneira seqüencial; Tratamento 1. nos dias 5 e 30 e Tratamento 2. nos dias 15 e 45 do período pós-operatório permitindo uma comparação na diferenciação epitelial e no reparo das feridas. Resultados: O enriquecimento das plaquetas obtido através de uma primeira centrifugação usando 300 g por 10 minutos e uma segunda 640 g por 10 minutos acelerou quatro vezes a reparação tecidual em relação ao controle. Conclusão: As feridas tratadas com gel de PRP apresentaram uma mais rápida diferenciação epitelial e acelerou a organização do colágeno da derme comparado ao grupo controle em cavalos. Descritores: Plasma Rico em Plaquetas. Cicatrização de Feridas. Pele. Cavalos.

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Section: Resultsmentioning

confidence: 99%

Effects of platelet-rich plasma gel on skin healing in surgical wound in horses

DeRossi¹,

Coelho²,

Mello³

et al. 2009

Acta Cir. Bras.

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“…[4][5][6][7][8][9] Thrombin receptor agonists have been shown to speed wound healing in animal models. [10][11][12][13] Fibrin also plays a critical role in wound healing. It forms the framework on which tissue repair takes place, and fibrin degradation products promote influx of neutrophils [14][15][16] and monocytes/macrophages.…”

Section: Introductionmentioning

confidence: 99%

Cutaneous wound healing is impaired in hemophilia B

Hoffman

Harger

Lenkowski

et al. 2006

Blood

102

145

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We used a mouse model to test the hypothesis that the time course and histology of wound healing is altered in hemophilia B. Punch biopsies (3 mm) were placed in the skin of normal mice and mice with hemophilia. The size of the wounds was measured daily until the epidermal defect closed. All wounds closed in mice with hemophilia by 12 days, compared with 10 days in normal animals. Skin from the area of the wound was harvested at different time points and examined histologically. Hemophilic animals developed subcutaneous hematomas; normal animals did not. Macrophage infiltration was significantly delayed in hemophilia B. Unexpectedly, hemophilic mice developed twice as many blood vessels in the healing wounds as controls, and the increased vascularity persisted for at least 2 weeks. The deposition and persistence of ferric iron was also greater in hemophilic mice. We hypothesize that iron plays a role in promoting excess angiogenesis after wounding as it had been proposed to do in hemophilic arthropathy. We have demonstrated that impaired coagulation leads to delayed wound healing with abnormal histology. Our findings have significant implications for treatment of patients with hemophilia, and also highlight the importance of rapidly establishing hemostasis following trauma or surgery. (Blood. 2006; 108:3053-3060)

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“…Moreover, PAR-1 is expressed in both the epidermis and dermis of normal and hypertrophic scars and in keloid lesions (12). The functional consequence of PAR-1 expression in the skin remains elusive however although accelerated wound healing in mice after topical PAR-1 activation pinpoints PAR-1 as an important receptor in the skin (13).…”

Section: Introductionmentioning

confidence: 99%

Protease Activated Receptor-1 Deficiency Diminishes Bleomycin-Induced Skin Fibrosis

Duitman

Ruela-de-Sousa

Shi

et al. 2014

Mol Med

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Accumulating evidence shows that protease-activated receptor-1 (PAR-1) plays an important role in the development of fibrosis, including lung fibrosis. However, whether PAR-1 also plays a role in the development of skin fibrosis remains elusive. The aim of this study was to determine the role of PAR-1 in the development of skin fibrosis. To explore possible mechanisms by which PAR-1 could play a role, human dermal fibroblasts and keratinocytes were stimulated with specific PAR-1 agonists or antagonists. To investigate the role of PAR-1 in skin fibrosis, we subjected wild-type and PAR-1-deficient mice to a model of bleomycin-induced skin fibrosis. PAR-1 activation leads to increased proliferation and extra cellular matrix (ECM) production, but not migration of human dermal fibroblasts (HDF) in vitro. Moreover, transforming growth factor (TGF)-β production was increased in keratinocytes upon PAR-1 activation, but not in HDF. The loss of PAR-1 in vivo significantly attenuated bleomycin-induced skin fibrosis. The bleomycin-induced increase in dermal thickness and ECM production was reduced significantly in PAR-1-deficient mice compared with wild-type mice. Moreover, TGF-β expression and the number of proliferating fibroblasts were reduced in PAR-1-deficient mice although the difference did not reach statistical significance. This study demonstrates that PAR-1 contributes to the development of skin fibrosis and we suggest that PAR-1 potentiates the fibrotic response mainly by inducing fibroblast proliferation and ECM production.

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Immobilized Thrombin Receptor Agonist Peptide Accelerates Wound Healing in Mice

Cited by 40 publications

References 24 publications

Effects of platelet-rich plasma gel on skin healing in surgical wound in horses

Effects of platelet-rich plasma gel on skin healing in surgical wound in horses

Cutaneous wound healing is impaired in hemophilia B

Protease Activated Receptor-1 Deficiency Diminishes Bleomycin-Induced Skin Fibrosis

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