Immobilized proteases for wound cleaning

Vernikovskii, B. V.; Степанова, Э. Ф.

doi:10.1134/s107036321203036x

Cited by 5 publications

(2 citation statements)

References 7 publications

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“…Trypsin is a serine protease enzyme usually found in digestive systems to hydrolyze proteins. Trypsin cleaves peptide chains mainly at the carboxyl side of lysine and arginine amino acids and breaks down the protein macromolecule [51]. Trypsin was the second probe enzyme to be tested in this silica and silica-cellulose matrices.…”

Section: Introductionmentioning

confidence: 99%

Potentials of Cellulose and Silica-based Materials for Enzyme Immobilization

Wonorahardjo

Suharti

Yuniawati

et al. 2022

Preprint

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This research aims to develop biomaterials for a place for enzyme immobilization in a designed porous matrix. Hydrolyzed bacterial cellulose was entrapped in the silica rice husk surface during gelling to modify the surface properties. Immobilized enzymes were investigated for their life duration and reusability. In this research, cellulase, trypsin, and lipase enzymes were used as probe enzymes to be immobilized in the silica-cellulose matrix, and their activity was tested using spectroscopic techniques. The systems exhibited good reusability up to 6 cycles with the also interesting outcome from the dynamics in the interface, which were varied by temperature, pH, and stirring speed. Silica-cellulose was also compared to silica alone in this experiment. When physical interaction with surfaces was assumed, enzyme activity decreased to 10, 20, and 40% due to confinement but underwent complex dynamics due to speedy adsorption-desorption equilibrium.

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Section: Introductionmentioning

confidence: 99%

Potentials of Cellulose and Silica-based Materials for Enzyme Immobilization

Wonorahardjo

Suharti

Yuniawati

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Some proteases that are able to catalyze the degradation of tissue proteins favor wound cleaning and also exhibit anti-inflammatory, fibrinolytic, and antiedemic effects (Vernikovskii and Stepanova, 2012).…”

Section: Introductionmentioning

confidence: 99%