Immobilized pH gradients

Gianazza, Elisabetta; Righetti, Pier Giorgio

doi:10.1002/elps.200800641

Cited by 17 publications

(11 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5). Since proteomics techniques have often been the subject of discussion in numerous reviews 20, 21–23, 65–67 and in a comprehensive book dedicated solely to plant proteomics 18, we briefly discuss below a few modified strategies that were undertaken for investigating plant secretomes.…”

Section: Technology: a Cocktail Of Biochemistry Proteomics And Biomentioning

confidence: 99%

Plant secretome: Unlocking secrets of the secreted proteins

Agrawal¹,

Jwa²,

Lebrun³

et al. 2010

Proteomics

268

252

View full text Add to dashboard Cite

Plant secretomics is a newly emerging area of the plant proteomics field. It basically describes the global study of secreted proteins into the extracellular space of plant cell or tissue at any given time and under certain conditions through various secretory mechanisms. A combination of biochemical, proteomics and bioinformatics approaches has been developed to isolate, identify and profile secreted proteins using complementary in vitro suspension-cultured cells and in planta systems. Developed inventories of secreted proteins under normal, biotic and abiotic conditions revealed several different types of novel secreted proteins, including the leaderless secretory proteins (LSPs). On average, LSPs can account for more than 50% of the total identified secretome, supporting, as in other eukaryotes, the existence of novel secretory mechanisms independent of the classical endoplasmic reticulum-Golgi secretory pathway, and suggesting that this non-classical mechanism of protein expression is, for as yet unknown reasons, more massively used than in other eukaryotic systems. Plants LSPs, which seem to be potentially involved in the defense/stress responses, might have dual (extracellular and/or intracellular) roles as most of them have established intracellular functions, yet presently unknown extracellular functions. Evidence is emerging on the role of glycosylation in the apical sorting and trafficking of secretory proteins. These initial secretome studies in plants have considerably advanced our understanding on secretion of different types of proteins and their underlying mechanisms, and opened a door for comparative analyses of plant secretomes with those of other organisms. In this first review on plant secretomics, we summarize and discuss the secretome definition, the applied approaches for unlocking secrets of the secreted proteins in the extracellular fluid, the possible functional significance and secretory mechanisms of LSPs, as well as glycosylation of secreted proteins and challenges involved ahead. Further improvements in existing and developing strategies and techniques will continue to drive forward plant secretomics research to building comprehensive and confident data sets of secreted proteins. This will lead to an increased understanding on how cells couple the concerted action of secreted protein networks to their internal and external environments.

show abstract

Section: Technology: a Cocktail Of Biochemistry Proteomics And Biomentioning

confidence: 99%

Plant secretome: Unlocking secrets of the secreted proteins

Agrawal¹,

Jwa²,

Lebrun³

et al. 2010

Proteomics

268

252

View full text Add to dashboard Cite

show abstract

“…In 2015, it will be 40 years since the advent of proteomics, revolutionized via the introduction of two‐dimensional gel electrophoresis (2‐DGE) (Klose, ; O'Farrell, ; Scheele, ) and later refined with the introduction of immobilized pH gradients (IPGs) (Bjellqvist et al, ; Righetti et al, ; Gianazza & Righetti, ; Görg et al, ) and MS (Aebersold & Mann, ; Yates, Ruse, & Nakorchevsky, ). Forty years can be considered a long time, but the fact is that the study of proteins within the term proteomics (Wilkins et al, ) is quite young, fluid, and diversifying as a technology.…”

Section: Plant Proteomics and Mass Spectrometry: A Decadementioning

confidence: 99%

A decade of plant proteomics and mass spectrometry: Translation of technical advancements to food security and safety issues

Agrawal¹,

Sarkar

Righetti

et al. 2013

Mass Spectrometry Reviews

Self Cite

View full text Add to dashboard Cite

Agrawal, G.K. et al. (2013). A decade of plant proteomics and mass spectrometry: Translation of technical advancements to food security and safety issues. AbstractTremendous progress in plant proteomics driven by mass spectrometry (MS) techniques has been made since 2000 when few proteomics reports were published and plant proteomics was in its infancy. These achievements include the refinement of existing techniques and the search for new techniques to address food security, safety, and health issues. It is projected that in 2050, the world's population will reach 9-12 billion people demanding a food production increase of 34-70% (FAO, 2009) from today's food production. Provision of food in a sustainable and environmentally committed manner for such a demand without threatening natural resources, requires that agricultural production increases significantly and that postharvest handling and food manufacturing systems become more efficient requiring lower energy expenditure, a decrease in postharvest losses, less waste generation and food with longer shelf life. There is also a need to look for alternative protein sources to animal based (i.e., plant based) to be able to fulfill the increase in protein demands by 2050. Thus, plant biology has a critical role to play as a science capable of addressing such challenges. In this review, we discuss proteomics especially MS, as a platform, being utilized in plant biology research for the past 10 years having the potential to expedite the process of understanding plant biology for human benefits. The increasing application of proteomics technologies in food security, analysis, and safety is emphasized in this review. But, we are aware that no unique approach/technology is capable to address the global food issues. Proteomics-generated information/resources must be integrated and correlated with other omics-based approaches, information, and conventional programs to ensure sufficient food and resources for human development now and in the future

show abstract

“…Reports compiled in Supporting Information Table 1 also indicate that 2‐DE alone accounts for 75% of the proteomics approaches used to investigate the rice proteomes. Several excellent reviews on 2‐DE are the evidence that covers in detail the history, application, sample solubilization, protein detection using different types of dyes, identification by MS, improvements such as differential in‐gel electrophoresis (DIGE), characterization of membrane and extreme proteins, strength, weakness, and future prospects; hence readers are recommended to those reviews 63–72. For example, the 2‐D DIGE technique requires low amount of protein (0.025–0.050 mg) compared to 2‐DE (ca.…”

Section: Technique Of Choice: Classical Protein Extraction and Sepamentioning

confidence: 99%

Rice proteomics: A move toward expanded proteome coverage to comparative and functional proteomics uncovers the mysteries of rice and plant biology

Agrawal¹,

Rakwal

2011

Proteomics

View full text Add to dashboard Cite

Growing rice is an important socio-economic activity. Rice proteomics has achieved a tremendous progress in establishing techniques to proteomes of almost all tissues, organs, and organelles during the past one decade (year 2000-2010). We have compiled these progresses time to time over this period. The present compilation discusses proteomics research in rice published between 1st April 2008 and 30th July 2010. Progress continues mainly towards protein cataloging deep into the proteome with high-confident protein assignment and some functional significance than ever before by (i) identifying previously unreported/low-abundance proteins, (ii) quantifying relative/absolute values of proteins, (iii) assigning protein responses to biotic/abiotic stresses, (iv) protein localization into organelles, (v) validating previous proteomes and eliminating false-positive proteins, and (vi) discovering potential biomarkers for tissues, organs, organelles, and for screening transgenic plants and food-safety evaluation. The notable achievements in global mapping of phosphorylation sites and identifying several novel secreted proteins into the extracellular space are worth appreciating. Our ever-increasing knowledge on the rice proteomics is beginning to impact the biology of not only rice, but also crops and plants. These major achievements will be discussed in this review keeping in mind newcomers, young, and established scientists in proteomics and plants.

show abstract

Immobilized pH gradients

Cited by 17 publications

References 112 publications

Plant secretome: Unlocking secrets of the secreted proteins

Plant secretome: Unlocking secrets of the secreted proteins

A decade of plant proteomics and mass spectrometry: Translation of technical advancements to food security and safety issues

Rice proteomics: A move toward expanded proteome coverage to comparative and functional proteomics uncovers the mysteries of rice and plant biology

Contact Info

Product

Resources

About