A decade of plant proteomics and mass spectrometry: Translation of technical advancements to food security and safety issues

Abstract

Agrawal, G.K. et al. (2013). A decade of plant proteomics and mass spectrometry: Translation of technical advancements to food security and safety issues. AbstractTremendous progress in plant proteomics driven by mass spectrometry (MS) techniques has been made since 2000 when few proteomics reports were published and plant proteomics was in its infancy. These achievements include the refinement of existing techniques and the search for new techniques to address food security, safety, and health issues. It is projected that in 2050, the world's population will reach 9-12 billion people demanding a food production increase of 34-70% (FAO, 2009) from today's food production. Provision of food in a sustainable and environmentally committed manner for such a demand without threatening natural resources, requires that agricultural production increases significantly and that postharvest handling and food manufacturing systems become more efficient requiring lower energy expenditure, a decrease in postharvest losses, less waste generation and food with longer shelf life. There is also a need to look for alternative protein sources to animal based (i.e., plant based) to be able to fulfill the increase in protein demands by 2050. Thus, plant biology has a critical role to play as a science capable of addressing such challenges. In this review, we discuss proteomics especially MS, as a platform, being utilized in plant biology research for the past 10 years having the potential to expedite the process of understanding plant biology for human benefits. The increasing application of proteomics technologies in food security, analysis, and safety is emphasized in this review. But, we are aware that no unique approach/technology is capable to address the global food issues. Proteomics-generated information/resources must be integrated and correlated with other omics-based approaches, information, and conventional programs to ensure sufficient food and resources for human development now and in the future

Advances in proteomic technologies and their scope of application in understanding plant–pathogen interactions

“…The term phyto-pathoproteomics can be defined as the application of proteomic tools to profile the proteome (total proteins) of pathogen, host and their interactions for understanding different aspects of phytopathology such as pathogenesis, plant disease resistance and molecular interface of plant-pathogen interactions. For a comprehensive history and development of proteomics, the readers can refer elsewhere (Patterson and Aebersold 2003;Thelen 2007;Agrawal et al 2013). …”

10 Standard abbreviations

“…A first such review on the subjecttranslational plant proteomics -was published in the Journal of Proteomics [7], followed by two reviews focusing on the application and translational aspects of plant proteomics in the new fields of nanoproteomics [8] and biofuel crops [9]. Another comprehensive review with particular focus towards plant proteomics application in food security and safety issues was published in Mass Spectrometry Reviews [10].…”

Zeus, Aesculapius, Amalthea and the proteome of goat milk

“…Thus, in 2011 Hettinga et al [2] reported as many as 192 proteins and Le et al [3] listed as many as 293 unique species, by resorting to various pre-fractionation techniques. An interesting study has also been published by A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 7 (per 100 mL) of the Roche cocktail of five protease inhibitors. Prior to CPLL treatment, one liter of milk was first delipidated by centrifugation and then casein depleted by precipitation at pH 4.6.…”