Immobilized metal affinity chromatography of histidine-tagged lentiviral vectors using monolithic adsorbents

Cheeks, Matthew; Kamal, Nabiha; Sorrell, A.; Darling, David; Farzaneh, Farzin; Slater, Nigel K.H.

doi:10.1016/j.chroma.2008.08.029

Cited by 48 publications

(26 citation statements)

References 29 publications

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“…Bioaffinity chromatography using cryogel monolith with immobilized concanavalin A [195] and Ni(II) ions [196] were also demonstrated. However, the binding capacity of these monoliths is inferior to those found for rigid monoliths [196]. The small surface area also makes cryogel monolith less suited for the separations of smaller entities such as proteins in adsorption modes.…”

Section: Cryogelsmentioning

confidence: 99%

Porous polymer monoliths: Amazingly wide variety of techniques enabling their preparation

Švec

2010

Journal of Chromatography A

535

389

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The porous polymer monoliths went a long way since their invention two decades ago. While the first studies applied the traditional polymerization processes at that time well established for the preparation of polymer particles, creativity of scientists interested in the monolithic structures has later led to the use of numerous less common techniques. This review article presents vast variety of methods that have meanwhile emerged. The text first briefly describes the early approaches used for the preparation of monoliths comprising standard free radical polymerizations and includes their development up to present days. Specific attention is paid to the effects of process variables on the formation of both porous structure and pore surface chemistry. Specific attention is also devoted to the use of photopolymerization. Then, several less common free radical polymerization techniques are presented in more detail such as those initiated by γ-rays and electron beam, the preparation of monoliths from high internal phase emulsions, and cryogels. Living processes including stable free radicals, atom transfer radical polymerization, and ring opening metathesis polymerization are also discussed. The review ends with description of preparation methods based on polycondensation and polyaddition reactions as well as on precipitation of preformed polymers affording the monolithic materials.

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Section: Cryogelsmentioning

confidence: 99%

Porous polymer monoliths: Amazingly wide variety of techniques enabling their preparation

Švec

2010

Journal of Chromatography A

535

389

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“…This was performed using murine 32Dp210 cells plated in RPMI, 10% FCS, 1% P/S and polybrene at 4.4 μg/ml as in [6]. A Becton Dickinson FACScan was used to read the GFP fluorescence.…”

Section: Methodsmentioning

confidence: 99%

Optimised concentration and purification of retroviruses using membrane chromatography

McNally

Darling

Farzaneh

et al. 2014

Journal of Chromatography A

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HighlightsAn in investigation in to the use of membrane chromatography for the purification of a γ-retrovirus was undertaken.The first report of a capacity for γ-retrovirus binding to a membrane chromatography device is presented.A process that produces a large increase in concentration and purity of the studied γ-retrovirus was identified.Proteomic techniques were used to identify the protein impurities removed and co-purified with the virus containing eluate.

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“…Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions INTRODUCTION Immobilized metal affinity chromatography (IMAC), also known as metal chelate affinity chromatography (MCAC), was first proposed by Porath et al (1) in 1975. The method has been widely used in the isolation and purification of proteins due to the selective affinity of metal chelate column for proteins and peptides (2)(3)(4)(5)(6). A series of immobilized metal chelate ligands was developed in order to improve the selectivity and stability of metal chelate column (4,(7)(8).…”

Section: Please Scroll Down For Articlementioning

confidence: 99%

A Novel Silica-Based Metal Chelate Stationary Phase—L-Glutamic Acid–Copper(II)

Wang

Chen

et al. 2010

Separation Science and Technology

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A novel silica-based metal chelate stationary phase, L-glutamic acid-copper(II) (L-Glu-Cu(II)), was synthesized. Effects of the immobilized metal ion, the pH of the solution, and competitive agents on chromatographic behavior of proteins were investigated on prepared chelate column. The coordination role of proteins on L-Glu-Cu(II) column was demonstrated. Optimal separation conditions for proteins on L-Glu-Cu(II) column were discussed. According to the established chromatographic conditions, protein mixtures were effectively separated. Separation performance of proteins and the leakage of Cu 2þ on L-Glu-Cu(II) column were discussed and compared with the traditional iminodiacetic acid-copper(II) (IDACu(II)) column. Separation performance of L-Glu-Cu(II) column for proteins was superior to that of the IDA-Cu(II) column. Under a proper eluting condition, a less amount of Cu 2þ was leaked from the L-Glu-Cu(II) column as compared with IDA-Cu(II) column. INTRODUCTIONImmobilized metal affinity chromatography (IMAC), also known as metal chelate affinity chromatography (MCAC), was first proposed by Porath et al. (1) in 1975. The method has been widely used in the isolation and purification of proteins due to the selective affinity of metal chelate column for proteins and peptides (2-6). A series of immobilized metal chelate ligands was developed in order to improve the selectivity and stability of metal chelate column (4,7-8). IDA is one of the most commonly used ligands since it has a suitable coordination number of atoms. This characteristic can meet not only with the coordination of immobilized metal ion, but also with the requirement of protein coordination owing to the remainder empty valence electron orbits of immobilized metal ion. When the transition metal ion, e.g., Ni 2þ , Co 2þ , or Zn 2þ , is immobilized on IDA, a weak affinity metal chelate

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Immobilized metal affinity chromatography of histidine-tagged lentiviral vectors using monolithic adsorbents

Cited by 48 publications

References 29 publications

Porous polymer monoliths: Amazingly wide variety of techniques enabling their preparation

Porous polymer monoliths: Amazingly wide variety of techniques enabling their preparation

Optimised concentration and purification of retroviruses using membrane chromatography

A Novel Silica-Based Metal Chelate Stationary Phase—L-Glutamic Acid–Copper(II)

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