Immobilized lipase catalyzed synthesis of n‐amyl acetate: parameter optimization, heterogeneous kinetics, continuous flow operation and reactor modeling

Abstract

BACKGROUND The work describes the synthesis of n‐amyl acetate from n‐amyl alcohol, a byproduct of the sugar industry. The synthesis was carried out using Burkholderia cepacia lipase immobilized on a hydrophobic polymer support containing hydroxypropyl methyl cellulose and polyvinyl alcohol. The reaction conditions were optimized and intrinsic kinetics were evaluated. An immobilized lipase‐coated film reactor was designed and evaluated for continuous flow synthesis. An attempt has been made to model the coated film reactor based on axial dispersion method. RESULTS The reaction conditions were optimized using a central composite design‐based response surface method. The optimum conditions for a maximum conversion of 99.5% were an acyl donor : alcohol ratio of 2:1, temperature 50 °C and catalyst loading 40 mg in n‐hexane. The intrinsic kinetics of reaction were fitted using an order bi–bi model with alcohol inhibition. The continuous flow synthesis was carried out in a coated film reactor with residence times ranging from 12 min to 60 min with per pass conversions of 5.6% and 22%, respectively. The catalyst was successfully recycled for five cycles. CONCLUSION The activation energy of the reaction was found to be 15.48 kJ mol−1. The productivity from batch and continuous flow reactor were found to be 8.16 and 6.54 mmol g−1 h−1. © 2018 Society of Chemical Industry

Analytical Determination of the Optimal Feed Temperature for Hydrogen Peroxide Decomposition Process Occurring in Bioreactor with a Fixed-Bed of Commercial Catalase

“…Despite the extensive literature on theoretical and experimental analyses of the HPD process, both the knowledge of the effect of operational conditions on bioreactor behaviour-especially temperature in case of batch processes and feed temperature in case of continuous processes-and development of mathematical methods applied to assess an optimal control variables values ensuring high bioreactor performance are required for efficient design of immobilized enzyme reactor [29,30].…”

Application of Immobilized Enzymes in Flow Biocatalysis for Efficient Synthesis

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