Immobilized enzyme electrode for the determination of l-lactate in food samples

Villamil, M.J.Fernández; Ordieres, Arturo J. Miranda; Blanco, Paulino Tuñón

doi:10.1016/s0003-2670(97)00046-9

Cited by 14 publications

(12 citation statements)

References 30 publications

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“…Lactate, a biochemical compound produced from pyruvate, is studied in various fields such as food preservation, biosensor and microbial fermentation [1][2][3][4]. Sulfites, benzoates, propionates, nitrites and nitrates are the commonly used preservatives for food products [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that these chemicals induce adverse effects in human such as stomach cancer and acidity [4][5][6][7]. Due to these adverse effects, lactate is used as a preservative for food products related to meat, poultry and fish [1][2][3][4]. The enhanced shelf life of food products is due to its antibacterial activity [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

“…Due to these adverse effects, lactate is used as a preservative for food products related to meat, poultry and fish [1][2][3][4]. The enhanced shelf life of food products is due to its antibacterial activity [1][2][3][4]. In 1990, Friedman [8] observed hepatic immaturity in children under the age of three when they were fed with food containing lactate.…”

Section: Introductionmentioning

confidence: 99%

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An electrochemical biosensor with nanointerface for lactate detection based on lactate dehydrogenase immobilized on zinc oxide nanorods

Nesakumar

Thandavan

Sethuraman

et al. 2014

Journal of Colloid and Interface Science

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An electrochemical biosensor with nanointerface for lactate detection based on lactate dehydrogenase immobilized on zinc oxide nanorods

Nesakumar

Thandavan

Sethuraman

et al. 2014

Journal of Colloid and Interface Science

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“…This value is comparable to the one reported in literature based on immobilization of lactate dehydrogenase and toluidine blue O by using a dialysis membrane and better than other two lactate biosensors where lactate oxidase was used as enzyme. 24,25 The analytical frequency was estimated to be 200 samples h -1 . The variability of measurements (inter-assay reproducibility) performed with three different biosensors was found to be 9%, demonstrating that differences in operator behavior to fabricate the biosensor are no relevant.…”

Section: Optimization Of the Biosensor Response And Analytical Paramementioning

confidence: 99%

A biosensor based on immobilization of lactate oxidase in a PB-CTAB film for FIA determination of lactate in beer samples

Lowinsohn

Bertotti

2008

J. Braz. Chem. Soc.

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No presente trabalho são apresentados resultados referentes à fabricação de um biossensor para análise de lactato. O dispositivo foi preparado imobilizando-se a enzima lactato oxidase em filme de Azul da Prússia (PB, sigla em inglês) depositado na superfície de eletrodo de carbono vítreo em soluções de CTAB. Nestas condições experimentais observou-se melhoria na sensibilidade e seletividade das determinações de lactato, as quais foram conduzidas em sistema FIA com detecção amperométrica. Nas condições otimizadas (pH = 6,9 e E = −0,1 V), obteve-se curva analítica com limite superior igual a 0,28 mmol L -1 e um limite de detecção de 0,84 µmol L -1 . A repetibilidade do método foi avaliada por meio de injeções consecutivas de amostras de lactato 0,28 mmol L -1 , obtendo-se desvio padrão de 2,2 % (n = 18). O método foi aplicado em amostras de cerveja e houve boa concordância entre os resultados obtidos e aqueles oriundos do uso de procedimento espectrofotométrico de referência.An amperometric lactate biosensor with lactate oxidase immobilized into a Prussian Blue (PB) modified electrode was fabricated. The advantage of using cetyltrimethylammonium bromide (CTAB) in the electrodeposition step of PB films onto glassy carbon surfaces was confirmed taking into account both the stability and sensitivity of the measurements. The biosensor was used in the development of a FIA amperometric method for the determination of lactate. Under optimal operating conditions (pH = 6.9, E = −0.1 V), the linear response of the method was extended up to 0.28 mmol L -1 lactate with a limit of detection of 0.84 µmol L -1 . The repeatability of the method for injections of a 0.28 mmol L -1 lactate solution was 2.2 % (n = 18). The usefulness of the method was demonstrated by determining lactate in beer samples and the results were in good agreement with those obtained by using a reference spectrophotometric enzyme method.Keywords: lactate, biosensor, prussian blue modified electrodes, CTAB, FIA amperometric detection, beer IntroductionThe relevance of metal hexacyanoferrates for electroanalytical chemistry was firstly demonstrated in the pioneering studies of Itaya et al. 1,2 involving the electrocatalysis of oxygen and hydrogen peroxide reduction at surfaces covered by Prussian Blue (PB, iron(III) hexacyanoferrate(II)) films. The transfer of electrons and the movement of small hydrated counter-cations through the channels and holes of the solid for charge compensation is a typical feature of this kind of compound. Owing to the zeolite nature of metal hexacyanoferrates, their ability as effective three-dimensional electrocatalysts has been extensively recognized.Once sufficient negative potentials are applied to PB modified electrodes, electrons are injected into the film and the so-called Prussian-White film is generated which arises from the reduction of a high-spin Fe 3+ . The reduced form (Fe 2+ ) is catalytically active towards the electron-transfer step involving hydrogen peroxide, water being formed as a final product. At positive pot...

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“…In foodstuffs, L ‐lactate monitoring is important for the control of the fermentation of cheese, yoghurt, butter, pickles, sauerkraut, wine, cider and other products. Therefore the reliable and rapid measurement of lactic acid is substantial in food industry 4. Many different analytical methods are now available for the determination of L ‐lactate with chromatographic, spectrophotometric, amperometric or by different enzymatic methods 1, but they are time‐ and labour‐intensive.…”

Section: Introductionmentioning

confidence: 99%

Determination of L‐Lactic Acid Content in Foods by Enzyme‐Based Amperometric Bioreactor

et al. 2011

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A thermostable lactate oxidase (from Aerococcus viridans) based bioreactor was elaborated for l-lactate determination in dairy products. The enzyme was immobilised in a thin-layer enzyme cell connected into a flow injection system with an amperometric detector. The linear measuring range was 0.1-1 mmol/L l-lactate (R 2 = 0.99; linear regression equation: y = 81.2x + 3.3). The detection limit was 0.05 mmol/L. 40 Samples were analyzed per hour. Considering reproducibility: the signals measured were very stable for over 800 injections, after this period the activity of the cell decreased slowly. The method was used for the control of dairy products and for monitoring milk freshness.

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Immobilized enzyme electrode for the determination of l-lactate in food samples

Cited by 14 publications

References 30 publications

An electrochemical biosensor with nanointerface for lactate detection based on lactate dehydrogenase immobilized on zinc oxide nanorods

An electrochemical biosensor with nanointerface for lactate detection based on lactate dehydrogenase immobilized on zinc oxide nanorods

A biosensor based on immobilization of lactate oxidase in a PB-CTAB film for FIA determination of lactate in beer samples

Determination of L‐Lactic Acid Content in Foods by Enzyme‐Based Amperometric Bioreactor

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