2008
DOI: 10.1590/s0103-50532008000400005
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A biosensor based on immobilization of lactate oxidase in a PB-CTAB film for FIA determination of lactate in beer samples

Abstract: No presente trabalho são apresentados resultados referentes à fabricação de um biossensor para análise de lactato. O dispositivo foi preparado imobilizando-se a enzima lactato oxidase em filme de Azul da Prússia (PB, sigla em inglês) depositado na superfície de eletrodo de carbono vítreo em soluções de CTAB. Nestas condições experimentais observou-se melhoria na sensibilidade e seletividade das determinações de lactato, as quais foram conduzidas em sistema FIA com detecção amperométrica. Nas condições otimizad… Show more

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Cited by 11 publications
(6 citation statements)
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“…These results are consistent with the reported literature on the activity of free LOx in solution, which indicates that the FcMe2-LPEI polymer used to immobilize the enzyme does not significantly interfere with its characteristic activity. (Lowinsohn and Bertotti 2008) Additionally, it should be noted that under physiological conditions (pH 7.4 and 37 ºC), the electrode film is within 85% of its maximum activity with respect to both temperature and pH. The ability to maintain activity under these conditions is crucial when considering the possibility of using FcMe2-LPEI/LOx as an implantable sensor.…”
Section: Electrochemical Characterization Of Lactate Sensormentioning
confidence: 99%
“…These results are consistent with the reported literature on the activity of free LOx in solution, which indicates that the FcMe2-LPEI polymer used to immobilize the enzyme does not significantly interfere with its characteristic activity. (Lowinsohn and Bertotti 2008) Additionally, it should be noted that under physiological conditions (pH 7.4 and 37 ºC), the electrode film is within 85% of its maximum activity with respect to both temperature and pH. The ability to maintain activity under these conditions is crucial when considering the possibility of using FcMe2-LPEI/LOx as an implantable sensor.…”
Section: Electrochemical Characterization Of Lactate Sensormentioning
confidence: 99%
“…Electrochemical experiments were carried out at room temperature by amperometric analysis with an applied potential of −0.10 V vs. the Ag/AgCl reference electrode. 59 The study of the LOX activity versus l -lactic acid was performed in a total volume of 100 μL of 0.1 M PBS buffer at pH 7, recording the current signals every 0.5 s. The fabricated biosensor must catalyze the oxidation of the substrate l -lactate into pyruvate. This reaction occurs in the presence of dissolved oxygen that oxidizes LOX by producing hydrogen peroxide which in turn is reduced by Prussian blue, which is finally reduced at the electrode surface (see Fig.…”
Section: Methodsmentioning
confidence: 99%
“…An amperometric sensor was also developed with lactate dehydrogenase which was immobilized into a Prussian blue-modified electrode for the detection of lactate with a LOD of 0.84 µM. The lactate detection in beer with this method performed better as compared with other commercial kits [118].…”
Section: Organic Acidsmentioning
confidence: 99%