Improved reuse and storage performances at room temperature of a new environmentally friendly lactate oxidase biosensor prepared by ambient electrospray immobilization

Abstract

A new, environmental-friendly, lactate oxidase (LOX) based biosensor for lactate detection, with unprecedented reuse and storage capabilities at room temperature, has been manufactured using the ambient electrospray deposition (ESD) technique....

Bioengineered Lactate Oxidase Mutants for Enhanced Electrochemical Performance at Acidic pH

“…On the other hand, Prussian blue (PB) was used as a mediator, which is known for electrochemical behavior towards H 2 O 2 , and shows biocompatibility properties [31] . Therefore, the bioelectrode construction is shown in Figure 3, where the initial oxidation of lactate to pyruvate is catalyzed by the AvLOx enzyme to generate H 2 O 2 , which is then reduced and detected under the electron transfer by the PB mediator within the working electrode [31,32] . Prussian blue is active in a wide pH range, including physiological values [33] .…”

“…[31] Therefore, the bioelectrode construction is shown in Figure 3, where the initial oxidation of lactate to pyruvate is catalyzed by the AvLOx enzyme to generate H 2 O 2 , which is then reduced and detected under the electron transfer by the PB mediator within the working electrode. [31,32] Prussian blue is active in a wide pH range, including physiological values. [33] Lactate is selectively detected by the PB-modified and carbonbased transducer at a low potential of 0 V vs. SCE, [34] generating a reducing current that varies proportionally to added lactate concentration.…”

Robust hydrogel sensor with good mechanical properties, conductivity, anti-swelling ability, water tolerance and biocompatibility

“…The maximum tensile strain was set at 100%, the tensile rate was 50 mm min −1 , and the hydrogel sample was subjected to 10 consecutive tensile load-unload experiments. The dissipated energy (U hys ) of the hydrogel sample in J m −3 was calculated using eqn (5), where ε max is the maximum elongation at break after loading and ε max ′ is the maximum elongation at break after unloading.…”

“…Electrically conductive hydrogels with the ability to transduce diverse deformations and/or motions into electrical signals have drawn considerable attention in various fields, such as wearable devices, [1][2][3][4][5][6] biomedical applications, 3,4 electronic skin, 5,6 flexible supercapacitors, 7,8 and soft robots. 9,10 The conductivity of the conductive hydrogels is achieved via introducing conductive additives into the hydrogels.…”