Immobilized endo-xylanase of Aspergillus tamarii Kita: an interesting biological tool for production of xylooligosaccharides at high temperatures

Heinen, Paulo Ricardo; Pereira, Maria José Bistafa; Vargas-Rechia, Carem Gledes; Almeida, Paula Zaghetto de; Monteiro, Lummy Maria Oliveira; Pasin, Thiago Machado; Messias, Josana Maria; Cereia, Mariana; Kadowaki, Marina Kimiko; Jorge, João Atı́lio; Polizeli, M. L. T. M.

doi:10.1016/j.procbio.2016.11.021

Cited by 28 publications

(19 citation statements)

References 40 publications

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“…3). It seems that at this temperature an optimum synergy is reached between the positive effect of high temperatures in the release of sugars and in the increase of the hydrolysis reaction rates (Heinen et al, 2017), and the optimum growth temperature for B. subtilis, which is reported to be between 30 and 37°C (Korsten & Cook, 1996), and optimum temperature for xylanase production, around 35-37°C (Helianti et al, 2016;Irfan, Asghar, Nadeem, Nelofer, & Syed, 2018). For temperatures higher than 50°C, a decrease of the Y RS value was observed possibly due its negative effect on B. subtilis growth (Irfan et al, 2018).…”

Section: Optimization Of the Bsg Concentration Ph And Temperature Fomentioning

confidence: 98%

Single-step production of arabino-xylooligosaccharides by recombinant Bacillus subtilis 3610 cultivated in brewers’ spent grain

Amorim

Silvério

Silva

et al. 2018

Carbohydrate Polymers

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Brewers' spent grain (BSG) is an inexpensive and abundant brewery by-product that can be used to produce prebiotic arabino-xylooligosaccharides (AXOS). In this study, Bacillus subtilis 3610 was used, for the first time, to produce AXOS through direct fermentation of BSG. Additionally, the microorganism was genetically modified to improve the AXOS production. The xylanase gene xyn2 from Trichoderma reesei coupled with a secretion tag endogenous to B. subtilis was cloned in pDR111 and integrated into its chromosome. After optimization by experimental design, AXOS with a degree of polymerization ranging from 2 to 6 were obtained. The maximum production yield expressed in xylose equivalents per amount of BSG (54.2 ± 1.1 mg/g) represents an increase of 33% comparing to the wild type. When compared with the enzymatic hydrolysis process, single-step fermentation with B. subtilis proved to be a very promising low-cost strategy for the simultaneous production of AXOS and valorization of BSG.

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Section: Optimization Of the Bsg Concentration Ph And Temperature Fomentioning

confidence: 98%

Single-step production of arabino-xylooligosaccharides by recombinant Bacillus subtilis 3610 cultivated in brewers’ spent grain

Amorim

Silvério

Silva

et al. 2018

Carbohydrate Polymers

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“…Recently, a mesoporous silica based support immobilized endoxylanase has been reported to produce X2‐X5 of 3.39 ±0.13 mg ml −1 from BWX . Other immobilized xylanases such as glyoxylagarose immobilized endoxylanase of A. tamarii Kita, glyoxyl agarose immobilized endoxylanase of Streptomyces halstedii . agarose activated with glyoxal groups immobilized endoxylanase of B. subtilis endoxylanase and agarose beads activated with aldehyde groups immobilized commercial endoxylanase (D333MDP) have been reported to produce XOS of varying DP and yields.…”

Section: Resultsmentioning

confidence: 98%

Immobilization of Recombinant Endo‐1,4‐β‐xylanase on Ordered Mesoporous Matrices for Xylooligosaccharides Production

et al. 2019

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Xylooligosaccharides (XOS) are emerging prebiotics, widely used in food, medicine and health care. XOS are produced by hydrolysis of xylan by acid or endoxylanase enzyme. Xylanase hydrolysis is preferred over acid due to its high specificity and absence of formation of non‐toxic byproducts. Immobilized xylanase improves enzyme‐stability and efficacy of XOS production through its repeated use. Though there are a few xylanases immobilized on conventional organic polymers for XOS production, immobilization of xylanse on mesoporous inorganic materials have not been investigated for XOS production. In this study, the recombinant endo‐1, 4‐β‐D‐xylanase (XynC) of B.subtilis KCX006 was purified and immobilized on ordered mesoporous matrices of carbon (CMK‐3), silica (SBA‐15) and zirconia (ZMF‐127). The immobilized‐XynC was characterized and used for XOS production by recycling. The recovered activity (RA) of immobilized‐XynC varied between 55 to 84%. The maximum RA was observed for XynC immobilized on ZMF‐127 matrix. All immobilized‐XynC had optimum pH similar to that of free‐XynC. But all immobilized‐XynC gained broader temperature range (50‐65°C) for optimal catalytic activity when compared with the free‐XynC. Immobilization of XynC resulted in higher Km due to substrate diffusion limit. All immobilized‐XynC produced free‐XOS of DP 2–6 (X2‐X6) and substituted‐XOS from beechwood xylan and extracted crude xylans from sorghum stalks and sugarcane bagasse (SCB). The XynC immobilized on SBA‐15 produced higher proportions of X2‐X6 compared to ZMF‐127 and CMK‐3. The XynC immobilized on SBA‐15 and CMK‐3 retained higher activity of 85–93% after four batches of repeated use. The observed efficiency of XynC immobilized on CMK‐3 and SBA‐15 are higher than the reported values. The results showed that XynC immobilized on mesoporous carbon and silica matrices would be useful for production of XOS by enzyme recycling.

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“…The xylanolytic system is composed of endoxylanase (EC 3.2.1.8), β ‐xylosidase (EC 3.2.1.37), α ‐glucuronidase (EC 3.2.1.139), and α ‐arabinofuranosidase (EC 3.2.1.55), which cooperatively convert xylan into simple sugars 5,9,10 . Cellulases include endoglucanases (EC 3.2.1.4), cellobiohydrolases (CBH I and CBH II, EC 3.2.1.91), and β ‐glucosidases (EC 3.2.1.21), which work together to release glucose 5,11 .…”

Section: Introductionmentioning

confidence: 99%

Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy

Pinheiro

Horváth

Lundin

et al. 2021

Biofuels Bioprod Bioref

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Biofuels contribute environment‐friendly, renewable energy, minimizing dependence on fossil energy. The pretreatment of biomass is a practical step that accelerates and facilitates the hydrolysis of complex materials. This work aimed to screen, select, and study cocktail formulations for biomass hydrolysis, where the enzymes were provided both from a commercial source (Trichoderma reesei, Novozymes®) and through the cultivation of Aspergillus brasiliensis and Aspergillus tamarii Kita. Experimental mixture designs were used to optimize the enzymatic conversion of substrates into simple sugars. A crude extract rich in amylase (AAB) had a significant favorable influence on cornmeal hydrolysis by maximizing the yield of reducing sugars (RS) (173 μmol mL–1). Celluclast™, rich in cellulase, significantly affected the hydrolysis of banana peel, maximizing the RS yield (175 μmol mL–1). Variable degrees of enzyme synergism were evident from statistical analysis of the biomass hydrolysis. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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Immobilized endo-xylanase of Aspergillus tamarii Kita: an interesting biological tool for production of xylooligosaccharides at high temperatures

Cited by 28 publications

References 40 publications

Single-step production of arabino-xylooligosaccharides by recombinant Bacillus subtilis 3610 cultivated in brewers’ spent grain

Single-step production of arabino-xylooligosaccharides by recombinant Bacillus subtilis 3610 cultivated in brewers’ spent grain

Immobilization of Recombinant Endo‐1,4‐β‐xylanase on Ordered Mesoporous Matrices for Xylooligosaccharides Production

Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy

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