Immobilization strategies of photolyases: Challenges and perspectives for DNA repairing application

Ramírez, Nicolás; Serey, Marcela; Illanes, Andrés; Piumetti, Marco; Ottone, Carminna

doi:10.1016/j.jphotobiol.2020.112113

Cited by 12 publications

(13 citation statements)

References 104 publications

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“…Hence, it can be predicted that presence of various types of Phrs in cyanobacteria might enable them to survive and sustain even in higher intensities of harmful UVR in their natural habits. Cryptochrome/Phr has aroused great research interests, and numerous works on the photoreaction have been reported recently (Kavakli et al 2017;Rajneesh et al 2017;Ramírez et al 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Despite the various advantages and high activity, of cyanobacterial Phr, their application as bioactive compounds in skin products may be limited by the lack of long-term stability and sensitivity to environmental conditions (Ramírez et al 2021). The search of extremophilic enzymes of pharmaceutical interest in extreme ecosystems has yielded several important enzymes (Lloyd et al 2004)…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Phrs have immense ability of photorepair, hence, constitute an important ingredient in topical creams (Ramírez et al 2021). In medical field, Phrs are used for reversing skin damage, and in preventing diseases such as actinic keratosis, cancer and premature photoaging (Ramírez et al 2021). Hence, research should focus on studying new Phrs from organisms such as cyanobacteria inhabiting extreme environments, where these high irradiances of UVR plays a crucial role for the Phrs to have optimum activity in the process of photorepair.…”

Section: Introductionmentioning

confidence: 99%

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Computational studies on photolyase (Phr) proteins of cyanobacteria

et al. 2022

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Photolyases (Phrs) are enzymes that utilize blue/ultraviolet (UV-A) region of light for repairing UV-induced cyclopyramidine dimer. We have studied Phr groups by bioinformatic analyses as well as active-site and structural modeling. The analysis of 238 amino acid sequences from 85 completely sequenced cyanobacterial genomes revealed five classes of Phrs, i.e., CPD Gr I, 6-4 Phrs/cryptochrome, Cry-DASH, Fe-S bacteria Phrs, and a group having fewer number of amino acids (276-385) in length. Distribution of Phr groups in cyanobacteria belonging to the order Synechococcales was found to be influenced by the habitats of the organisms. Class V Phrs were exclusively present in cyanobacteria. Unique motif and binding sites were reported in Group II and III. Fe-S protein binding site was only present in Group V. Active site residues and putative CPD/6-4pp binding residues are charged amino acids which were present on the surface of the proteins. Majority of hydrophilic amino acid residues were present on surface of Phrs. Sequence analysis confirmed the diverse nature of Phrs, though, sequence diversity does not affect their overall 3D structure. Protein-ligand interaction analysis identified novel CPD/6-4PP binding sites on Phrs. This structural information of Phrs can be used for the preparation of efficient Phr based formulations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Computational studies on photolyase (Phr) proteins of cyanobacteria

et al. 2022

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“…Recombinant photolyase purified by affinity chromatography with immobilized metal has no impurities and shows high reactivity and resistance to high doses of UV [ 67 ]. Different variants of DNA photolyase immobilization on various nanostructured carriers for targeted delivery and stability of the molecule are also considered [ 68 ]. In addition, for example, palladium/platinum nanoparticles themselves can be used to eliminate the effects of photodamage and oxidative stress in cells [ 69 , 70 , 71 ].…”

Section: Dna Photolyases As a Tool Of Protection Against Photodamage Of Human Skin And Its Treatmentmentioning

confidence: 99%

UV Radiation in DNA Damage and Repair Involving DNA-Photolyases and Cryptochromes

et al. 2021

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Prolonged exposure to ultraviolet radiation on human skin can lead to mutations in DNA, photoaging, suppression of the immune system, and other damage up to skin cancer (melanoma, basal cell, and squamous cell carcinoma). We reviewed the state of knowledge of the damaging action of UVB and UVA on DNA, and also the mechanisms of DNA repair with the participation of the DNA-photolyase enzyme or of the nucleotide excision repair (NER) system. In the course of evolution, most mammals lost the possibility of DNA photoreparation due to the disappearance of DNA photolyase genes, but they retained closely related cryptochromes that regulate the transcription of the NER system enzymes. We analyze the published relationships between DNA photolyases/cryptochromes and carcinogenesis, as well as their possible role in the prevention and treatment of diseases caused by UV radiation.

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