UV Radiation in DNA Damage and Repair Involving DNA-Photolyases and Cryptochromes

Vechtomova, Yulia L.; Телегина, Т. А.; Buglak, Andrey A.; Kritsky, M. S.

doi:10.3390/biomedicines9111564

Cited by 30 publications

(16 citation statements)

References 81 publications

(146 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cyanobacteria do these works relying on sunlight as the energy source. Meanwhile, they suffer from the UV component of sunlight, which induces cyclobutane pyrimidine dimer (CPD, ∼70–90%) and the (6–4) pyrimidine-pyrimidone photoproduct (6–4 photoproduct, ∼10–30%) in DNA ( 4 , 5 ). These harmful lesions must be repaired to restore the normal functions of DNA.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of a prokaryotic 6-4 photolyase from Synechococcus elongatus with a deazariboflavin antenna chromophore

Chen

Liu

Zhou

et al. 2022

Nucleic Acids Research

View full text Add to dashboard Cite

Synechococcus elongatus, formerly known as Anacystis nidulans, is a representative species of cyanobacteria. It is also a model organism for the study of photoreactivation, which can be fully photoreactivated even after receiving high UV doses. However, for a long time, only one photolyase was found in S. elongatus that is only able to photorepair UV induced cyclobutane pyrimidine dimers (CPDs) in DNA. Here, we characterize another photolyase in S. elongatus, which belongs to iron-sulfur bacterial cryptochromes and photolyases (FeS-BCP), a subtype of prokaryotic 6–4 photolyases. This photolyase was named SePhrB that could efficiently photorepair 6–4 photoproducts in DNA. Chemical analyses revealed that SePhrB contains a catalytic FAD cofactor and an iron-sulfur cluster. All of previously reported FeS-BCPs contain 6,7-dimethyl-8-ribityllumazine (DMRL) as their antenna chromophores. Here, we first demonstrated that SePhrB possesses 7,8-didemethyl-8-hydroxy-5-deazariboflavin (8-HDF) as an antenna chromophore. Nevertheless, SePhrB could be photoreduced without external electron donors. After being photoreduced, the reduced FAD cofactor in SePhrB was extremely stable against air oxidation. These results suggest that FeS-BCPs are more diverse than expected which deserve further investigation.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification and characterization of a prokaryotic 6-4 photolyase from Synechococcus elongatus with a deazariboflavin antenna chromophore

Chen

Liu

Zhou

et al. 2022

Nucleic Acids Research

View full text Add to dashboard Cite

show abstract

“…Lipids, nucleic acids, and proteins are ROS , RNS, and reactive sulfur species ( RSS ) targets [ 63 ]. UV -A damages DNA indirectly by generating ROS -like radical singlet oxygen (1O2) and/or hydrogen peroxide among others, causing DNA mutations during the replication process such as the guanine–thiamine (G–T) transversion, while UV-B directly damages DNA strands, generating cyclobutane pyrimidine dimers ( CPDs ) by covalently bonding two neighboring pyrimidines; their accumulation over time can produce an interruption of DNA replication and transcription, disturbing the function of the damaged cell [ 62 , 64 ]. Over the last two decades, studies have been conducted to find and obtain antioxidants from natural sources in order to replace synthetic sources to treat oxidative damage.…”

Section: Antioxidantsmentioning

confidence: 99%

Microalgae Bioactive Compounds to Topical Applications Products—A Review

et al. 2022

View full text Add to dashboard Cite

Microalgae are complex photosynthetic organisms found in marine and freshwater environments that produce valuable metabolites. Microalgae-derived metabolites have gained remarkable attention in different industrial biotechnological processes and pharmaceutical and cosmetic industries due to their multiple properties, including antioxidant, anti-aging, anti-cancer, phycoimmunomodulatory, anti-inflammatory, and antimicrobial activities. These properties are recognized as promising components for state-of-the-art cosmetics and cosmeceutical formulations. Efforts are being made to develop natural, non-toxic, and environmentally friendly products that replace synthetic products. This review summarizes some potential cosmeceutical applications of microalgae-derived biomolecules, their mechanisms of action, and extraction methods.

show abstract

“…The photorepair quantum yield for CPD photolyases equals 0.7–1 (depending on the antenna and the efficiency of energy transfer from it to the flavin) and for (6-4) photolyases it is ca. 0.3 due to the more complex splitting mechanism of (6-4)PP [ 222 , 234 , 241 ].…”

Section: Biochemical and Physiological Application Of Pterin Photoche...mentioning

confidence: 99%

Insights into Molecular Structure of Pterins Suitable for Biomedical Applications

Buglak

Kapitonova

Vechtomova

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Pterins are an inseparable part of living organisms. Pterins participate in metabolic reactions mostly as tetrahydropterins. Dihydropterins are usually intermediates of these reactions, whereas oxidized pterins can be biomarkers of diseases. In this review, we analyze the available data on the quantum chemistry of unconjugated pterins as well as their photonics. This gives a comprehensive overview about the electronic structure of pterins and offers some benefits for biomedicine applications: (1) one can affect the enzymatic reactions of aromatic amino acid hydroxylases, NO synthases, and alkylglycerol monooxygenase through UV irradiation of H4pterins since UV provokes electron donor reactions of H4pterins; (2) the emission properties of H2pterins and oxidized pterins can be used in fluorescence diagnostics; (3) two-photon absorption (TPA) should be used in such pterin-related infrared therapy because single-photon absorption in the UV range is inefficient and scatters in vivo; (4) one can affect pathogen organisms through TPA excitation of H4pterin cofactors, such as the molybdenum cofactor, leading to its detachment from proteins and subsequent oxidation; (5) metal nanostructures can be used for the UV-vis, fluorescence, and Raman spectroscopy detection of pterin biomarkers. Therefore, we investigated both the biochemistry and physical chemistry of pterins and suggested some potential prospects for pterin-related biomedicine.

show abstract

UV Radiation in DNA Damage and Repair Involving DNA-Photolyases and Cryptochromes

Cited by 30 publications

References 81 publications

Identification and characterization of a prokaryotic 6-4 photolyase from Synechococcus elongatus with a deazariboflavin antenna chromophore

Identification and characterization of a prokaryotic 6-4 photolyase from Synechococcus elongatus with a deazariboflavin antenna chromophore

Microalgae Bioactive Compounds to Topical Applications Products—A Review

Insights into Molecular Structure of Pterins Suitable for Biomedical Applications

Contact Info

Product

Resources

About