Immobilization strategies of Brucella particles on optical fibers for use in chemiluminescence immunosensors

Liebes, Yael; Amir, Liron; Marks, Robert S.; Banai, Menachem

doi:10.1016/j.talanta.2009.06.070

Cited by 20 publications

(18 citation statements)

References 43 publications

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“…Liebes et al (2009) tested milk samples for antibodies to Brucella as an indicator of bovine pathogen exposure. They generated a chemiluminescent signal at the surface of an optical fiber coated with killed Brucella organisms.…”

Section: Veterinary Medicinementioning

confidence: 99%

Point-of-care diagnostics for niche applications

Cummins

Ligler

Walker

2016

Biotechnology Advances

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Point-of-care or point-of-use diagnostics are analytical devices that provide clinically relevant information without the need for a core clinical laboratory. In this review we define point-of-care diagnostics as portable versions of assays performed in a traditional clinical chemistry laboratory. This review discusses five areas relevant to human and animal health where increased attention could produce significant impact: veterinary medicine, space travel, sports medicine, emergency medicine, and operating room efficiency. For each of these areas, clinical need, available commercial products, and ongoing research into new devices are highlighted.

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Section: Veterinary Medicinementioning

confidence: 99%

Point-of-care diagnostics for niche applications

Cummins

Ligler

Walker

2016

Biotechnology Advances

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“…[ 57 ] Concentrations between 0.3 and 100 vol% can be applied for surface functionalization with silanization reagents. [ 58 ] Preliminary studies have shown that the materials become hydrophobic after using a 3 or 5 wt% 3‐(glycidoxypropyl)dimethylmethoxysilane solution, preventing their use in aqueous medium (Table S1, Supporting Information). For this reason, and because pore‐blocking should be avoided, a concentration of 1 wt% was used.…”

Section: Resultsmentioning

confidence: 99%

Influence of Pore Space Hierarchy on the Efficiency of an Acetylcholinesterase‐Based Support for Biosensorics

Enke

Anders

Tallarek

2020

Adv Materials Inter

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The influence of a hierarchically structured pore system of a silica sol–gel support for application as a functional component in an acetylcholinesterase‐based enzyme array, with respect to its efficiency (response time, in particular) is investigated. Careful adjustment of synthesis parameters and a novel drying method allow to prepare monolithic silica sol–gel membranes with monomodal or hierarchical pore structures. These supports enable direct comparison regarding the influence of morphological properties on maximum acetylcholinesterase (AChE) loading by a membrane and on the apparent reaction rate of the AChE‐catalyzed degradation of acetylcholine at identical enzyme loading. It is shown for the first time that the hierarchical, meso‐macroporous material is superior over the monomodal structures (of either mesopores or macropores) regarding combined functionality and transport efficiency, as reflected in the apparent reaction rates. The advantage of the mesopores in a hierarchical system is manifested in higher maximum enzyme loading than for purely macroporous material, while the presence of macropores results in less obstructed transport that for a purely mesoporous material, which in turn reduces the response time.

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“…As a starting point we used a previously described immobilization protocol (Protocol 1) 14 and optimized each step in order to maximize the chemiluminescence output.…”

Section: Resultsmentioning

confidence: 99%

Fiber-Optic Immunosensor for Detection of Crimean-Congo Hemorrhagic Fever IgG Antibodies in Patients

et al. 2015

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Crimean-Congo hemorrhagic fever (CCHF) is a severe viral disease with high fatality rate.CCHF virus is endemic in parts of Africa, Asia, Middle East and southeastern Europe.Rapid diagnostics of CCHF is vital for appropriate clinical management and prevention of secondary spread from human-to-human. Currently, diagnostics relies on Real-Time RT-PCR and antibody or antigen detection using ELISA. These methods require trained personnel and expensive equipment and are not appropriate for point-of-care (POC) diagnostics. Furthermore there are no POC assays available for CCHF.We developed a fiber-optic biosensor for the detection of CCHF IgG antibodies. In order to improve sensitivity, we optimized both the bioreceptor immobilization protocol and the chemiluminescence substrate formulation. The resulting protocol showed a 100-fold greater sensitivity for detection of CCHF antibodies. Finally, we evaluated the fiber-optic biosensor with two CCHF patient sera. We showed that the fiber-optic biosensor is 10-times more sensitive that colorimetric ELISA and is able to detect both patients with high and low levels of IgG antibodies. We believe that the fiber-optic biosensor is a suitable alternative to ELISA as it is much more sensitive and makes it possible to detect small amount of antibodies at an early stage of infection, and can be integrated as a point-of-care diagnostic system of CCHF.

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Immobilization strategies of Brucella particles on optical fibers for use in chemiluminescence immunosensors

Cited by 20 publications

References 43 publications

Point-of-care diagnostics for niche applications

Point-of-care diagnostics for niche applications

Influence of Pore Space Hierarchy on the Efficiency of an Acetylcholinesterase‐Based Support for Biosensorics

Fiber-Optic Immunosensor for Detection of Crimean-Congo Hemorrhagic Fever IgG Antibodies in Patients

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