Immobilization of β-cyclodextrin in chitosan beads for separation of cholesterol from egg yolk

Chiu, Shao Hua; Chung, Tsair-Wang; Giridhar, R.; Wu, Wen Teng

doi:10.1016/j.foodres.2003.12.001

Cited by 84 publications

(52 citation statements)

References 33 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chitosan macrospheres were prepared using a previously reported precipitation method [57]). Chitosan (2 w/v %) was dissolved in an aqueous solution of acetic acid (2 w/v %).…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

Chloroperoxidase-Mediated Halogenation of Selected Pharmaceutical Micropollutants

et al. 2018

View full text Add to dashboard Cite

Abstract:The oxidation of eight pharmaceutical micropollutants by chloroperoxidase derived from Caldaromyces fumago using hydrogen peroxide as an electron acceptor is reported. All the tested compounds, namely trazadone, sulfamethoxazole, naproxen, tetracycline, estradiol, ketoconazole, ketorolac, and diclofenac, were found to be substrates for oxidation by chloroperoxidase. The respective oxidation products were identified by electrospray ionization-mass spectrometry. All the products contain at least one chloride atom in their structure after the enzymatic oxidation. Degradability experiments indicated that most of the reaction products are more biodegradable than the corresponding unmodified compounds. The enzyme was found to be catalytically active in effluent from a water treatment facility, transforming the micropollutants with high reaction rates and conversions. The enzyme was immobilized in chitosan macrospheres, which allowed the catalyst to be recycled for up to three treatment cycles in simulated samples of treated residual water. The conversion was high in the first two cycles; however, in the third, a 50% reduction in the capacity of the enzyme to oxidize ketorolac was observed. Additionally, immobilization improved the performance of the enzyme over a wider pH range, achieving the conversion of ketorolac at pH 5, while the free enzyme was not active at this pH. Overall, the results of this study suggest that chloroperoxidase represents a powerful potential catalyst in terms of its catalytic activity for the transformation of pharmaceutical micropollutants.

show abstract

“…Chitosan macrospheres were prepared using a previously reported precipitation method [57]). Chitosan (2 w/v %) was dissolved in an aqueous solution of acetic acid (2 w/v %).…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

Chloroperoxidase-Mediated Halogenation of Selected Pharmaceutical Micropollutants

et al. 2018

View full text Add to dashboard Cite

show abstract

“…BCD was immobilized onto CS by cross-linking with HMDI reagent in two reaction steps with 2-ethylhexanoate as the catalyst (Chiu et al 2004). About 0.2 g of dry CS beads was placed in 10 ml of dimethylformamide (DMF) solution containing 2.5% (v/v) of HMDI.…”

Section: Immobilization Of β-Cyclodextrinmentioning

confidence: 99%

“…BCD has a polar cavity in the molecule and has the capability of forming inclusion complex with various compounds (Ahn and Kwak 1999;Chiu et al 2004;Prabaharan and Mano 2006). The major contamination of whey in the separation of GMP is the lipid.…”

Section: Introductionmentioning

confidence: 99%

The separation of GMP from milk whey using the modified chitosan beads

Song

Hein

et al. 2010

Adsorption

View full text Add to dashboard Cite

The cationic property of chitosan was used to adsorb glycomacropeptide (GMP) molecules from milk whey proteins. β-cyclodextrin was immobilized to native chitosan beads by cross-linking with 1,6-hexamethylene diisocyanate (HMDI). The resultant modified beads (CS-HMDI-BCD) presented superior adsorption affinity and capacity towards GMP. At pH 3.0, 90.23% of GMP was adsorbed with a maximum adsorption capacity corresponding to 12.87 mg of sialic acid/g-adsorbent. Desorption experiments showed that the modified beads could be regenerated and used in many cycles without significant decreases in the capacity and selectivity.

show abstract

“…Nowadays, there is a growing interest in the chemical modification of chitosan in order to improve its solubility or the cross-linking ability in different media and therefore widen its applications [51][52][53][54]. The reaction between chitosan and diisocyanate compounds has been previously promoted to produce bio-polyurethanes for the development of molecular carriers in the biomedical and environmental fields [55], beads for cholesterol absorption [56] or materials with controlled thermal or elastomeric properties [57,58]. As a continuation of the above mentioned research on the development of oleogels for lubricant purposes and taking into account the interest of these biopolymers, the effect of NCO-functionalization degree of chitin and chitosan on the rheological properties and thermal degradation of resulting chemical oleogels has been studied in this work.…”

Section: Introductionmentioning

confidence: 99%

Influence of Functionalization Degree on the Rheological Properties of Isocyanate-Functionalized Chitin- and Chitosan-Based Chemical Oleogels for Lubricant Applications

et al. 2014

View full text Add to dashboard Cite

This work deals with the influence of functionalization degree on the thermogravimetric and rheological behaviour of NCO-functionalized chitosan-and chitin-based oleogels. Chitosan and chitin were functionalized using different proportions of 1,6-hexamethylene diisocyanate (HMDI) and subsequently dispersed in castor oil to promote the chemical reaction between the -NCO group of the modified biopolymer and the -OH group located in the ricinoleic fatty acid chain of castor oil, thus resulting in different oleogels with specific thermogravimetric and rheological characteristics. Biopolymers and oleogels were characterized through Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). Small-amplitude oscillatory shear (SAOS) measurements were performed on the oleogels. Oleogels presented suitable thermal resistance, despite the fact that the inclusion of HMDI moieties in the polymer structure led to a reduction in the onset temperature of thermal degradation. The insertion of low amounts of HMDI in both chitin and chitosan produces a drastic reduction in the values of oleogel viscoelastic functions but, above a critical threshold, they increase with OPEN ACCESSPolymers 2014, 6 1930 the functionalization degree so that isocyanate functionalization results in a chemical tool to modulate oleogel rheological response. Several NCO-functionalized chitosan-and chitin-based oleogel formulations present suitable thermal resistance and rheological characteristics to be proposed as bio-based alternatives to traditional lubricating greases.

show abstract

Immobilization of β-cyclodextrin in chitosan beads for separation of cholesterol from egg yolk

Cited by 84 publications

References 33 publications

Chloroperoxidase-Mediated Halogenation of Selected Pharmaceutical Micropollutants

Chloroperoxidase-Mediated Halogenation of Selected Pharmaceutical Micropollutants

The separation of GMP from milk whey using the modified chitosan beads

Influence of Functionalization Degree on the Rheological Properties of Isocyanate-Functionalized Chitin- and Chitosan-Based Chemical Oleogels for Lubricant Applications

Contact Info

Product

Resources

About