Immobilization of β-1,3-xylanase on pitch-based hyper-crosslinked polymers loaded with Ni2+ for algal biomass manipulation

Liu, Ting; Pei, Baoyou; Lin, Jinqing; Zhang, Guangya

doi:10.1016/j.enzmictec.2020.109674

Cited by 8 publications

(1 citation statement)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 1,3‐xylanases immobilized on the hyper‐crosslinked polymer (HCP) material prepared by Liu et al. could release more reducing sugar [29] . However, most of these works focus on the adsorption capacity, but pay little attention to the activity of immobilized enzymes.…”

Section: Introductionmentioning

confidence: 99%

Selective Immobilization of His‐Tagged Phosphomannose Isomerase on Ni Chelated Nanoparticles with Good Reusability and Activity

et al. 2022

View full text Add to dashboard Cite

Self‐stable precipitation polymerization was used to prepare an enzyme‐immobilized microsphere composite. Phosphomannose isomerase (PMI) with His‐tag was successfully immobilized on Ni2+ charged pyridine‐derived particles. The maximum amount of PMI immobilized on such particles was ∼184 mg/g. Compared with free enzyme, the activity of the immobilized enzymes was significantly improved. In addition, the immobilized enzymes showed a much better thermostability than free enzymes. At the same time, the immobilized enzymes can be reused for multiple reaction cycles. We observed that the enzyme activity did not decrease significantly after six cycles. We conclude that the pyridine‐derived particles can be used to selectively immobilize His‐tagged enzymes, which can couple the enzyme purification and catalysis steps and improve the efficiency of enzyme‐catalyzed industrial processes.

show abstract

Section: Introductionmentioning

confidence: 99%