Immobilization of urease on vapour phase stain etched porous silicon

Maia, Maria de Mascena Diniz; Vasconcelos, Elder A. de; Maia, Paula Ferdinanda Conceição de Mascena Diniz; Maciel, Jackeline da Costa; Cajueiro, Karla Regina Rizzardi; Silva, María da Paz Carvalho da; Silva, Eronides Felisberto da; Dutra, Rosa F.; Freire, V. N.; Filho, José Luiz de Lima

doi:10.1016/j.procbio.2006.08.008

Cited by 25 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polyethyleneimine is used as a crosslinking agent by many research teams around the world [59][60][61][62]. An example of the use of PEI to activate a substrate for immobilisation is the system using urease to detect urea [63] or peroxidase to identify paracetamol [64]. The present results show that PEI can be successfully used for the activation of chitosan microspheres with the maintenance of the enzymatic activity of the immobilised biocatalyst.…”

Section: Immobilisation Of Enzymes On Chitosan Microspheresmentioning

confidence: 65%

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Sulej

Piątek-Gołda

Grąz

et al. 2023

JFB

View full text Add to dashboard Cite

Lactobionic acid (LBA) is a bioactive compound that has become increasingly popular in medicine in recent years due to its unique properties. This chemical can be formed via the enzymatic oxidation of lactose using fungal oxidoreductive enzymes. This study aimed to intensify the synthesis of LBA using immobilised enzymes (cellobiose dehydrogenase from Phanerochaete chrysosporium (PchCDH) and laccase from Cerrena unicolor (CuLAC)) on chitosan microspheres. We used three different crosslinking agents: genipin, glutaraldehyde, and polyethyleneimine to activate the chitosan. The FTIR and CellDrop techniques were used to characterise the activated microspheres. Quantitative (HPLC) and qualitative (TLC) methods were used to determine the obtained LBA. The results show that the type of activator used influences the efficiency of the binding of the enzyme to the matrix. Furthermore, the amount of LBA formed depends on the type of system used. The use of a system in which one of the enzymes is immobilised on a PEI-activated carrier (PchCDH) and the other is free (CuLAC) proved to be the most optimal, as it yielded almost 100% conversion of lactose to lactobionic acid. Summarising the data obtained the following: lactobionic acid immobilised on chitosan microspheres has great potential for medical applications.

show abstract

Section: Immobilisation Of Enzymes On Chitosan Microspheresmentioning

confidence: 65%

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Sulej

Piątek-Gołda

Grąz

et al. 2023

JFB

View full text Add to dashboard Cite

show abstract

“…An important requirement of many bioassay or drug delivery applications is that the collection, concentration, and immobilization processes not denature or otherwise deactivate the biomolecule of interest. While it has been demonstrated that porous Si matrices can release antibodies,29 enzymes,19, 30–36 or other biomolecules37 in their active form, some compositions of porous Si are known to undergo irreversible chemical reactions with drugs or other molecules 38–40. In the present case, the enzymatic activity of lysozyme provides a functional assay of the compatibility of the carbonized pSi opto‐electrochemical device with sensitive proteins.…”

mentioning

confidence: 90%

Electric‐Field‐Assisted Protein Transport, Capture, and Interferometric Sensing in Carbonized Porous Silicon Films

Chen¹,

Klunk²,

Diep³

et al. 2011

Advanced Materials

View full text Add to dashboard Cite

show abstract

“…• HNO 3 /HF vapor etching can be advantageous for biosensor fabrication due to reduction in cost and easy scalability (large-area PS layers can be easily fabricated) (Maia et al 2007). Fig.…”

Section: Diverse Uses Of Vapor Etchingmentioning

confidence: 99%

Porous Silicon Formation by HNO3/HF Vapor Etching

Bessaı̈s

2014

Handbook of Porous Silicon

View full text Add to dashboard Cite

Immobilization of urease on vapour phase stain etched porous silicon

Cited by 25 publications

References 13 publications

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Electric‐Field‐Assisted Protein Transport, Capture, and Interferometric Sensing in Carbonized Porous Silicon Films

Porous Silicon Formation by HNO3/HF Vapor Etching

Contact Info

Product

Resources

About