Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture

Tunma, Somruthai; Song, Dong Ho; Kim, Si Eun; Kim, Kyoung Nam; Han, Jeon G.; Boonyawan, Dheerawan

doi:10.1016/j.apsusc.2013.07.046

Cited by 11 publications

(5 citation statements)

References 22 publications

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“…Two absorption peaks at 276 and 216 nm were distinguished in the absorbance profile of the sample (Figure 2b), corresponding to aromatic amino acids and peptide bonds, respectively. Similar to previous reports, peptide bonds presented the highest absorbance, 34 confirming the minor representation of aromatic amino acids in SS. 32 Interestingly, the absorption peak for aromatic amino acids was registered at a wavelength very close to that reported for a commercial SS sample (275.4 nm), 35 ensuring the good quality of the SS sample.…”

Section: ■ Results and Discussionsupporting

confidence: 91%

Silk Sericin-Functionalized Bacterial Cellulose as a Potential Wound-Healing Biomaterial

et al. 2016

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Bacterial cellulose (BC) is a polysaccharide known as a suitable matrix for proper wound healing. To improve this ability, BC was functionalized with silk sericin (SS) that has cytoprotective and mitogenic effects. The composites obtained by solution impregnation were stabilized by hydrogen bonds, and SS could be released in a controlled manner. The constructs were highly porous with interconnected pores allowing for high water uptake that varied with the SS concentration used for sample preparation. While SS did not disrupt the stability of the BC network, soluble SS diffusing from the composites did not influence keratinocyte growth but enhanced fibroblast proliferation, which would further optimize the wound healing process and improve extracellular matrix production, accelerating healing. Further, improved cell viability was observed upon the composites. Because of their attractive structure and properties, these BC-SS biomaterials represent potential candidates not only for wound dressing applications but also for tissue engineering.

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Section: ■ Results and Discussionsupporting

confidence: 91%

Silk Sericin-Functionalized Bacterial Cellulose as a Potential Wound-Healing Biomaterial

et al. 2016

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“…It is clearly shown in Figure (b) that the water droplet sticks firmly onto the APP‐PSt CSPs, and the advancing contact angle increases to 83.2° with a higher droplet height 0.95 mm. The contact angle of PSt is 82 ± 1.4° according the published work . This means that PSt effectively shelled APP to form CSPs.…”

Section: Resultsmentioning

confidence: 57%

Flame retardant and mechanical properties of epoxy composites containing APP−PSt core−shell microspheres

Zhao

Xing

et al. 2013

J of Applied Polymer Sci

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Ammonium polyphosphate (APP)-polystyrene (PSt) core-shell microspheres (CSPs) were synthesized via in situ radical polymerization. The core-shell structure was confirmed by transmission electron microscope (TEM). The results of optical contact angle measurements demonstrated a significant improvement in hydrophobicity of the modified APP. The obtained APP-PSt CSPs were added into epoxy (EP) system with various loadings. Effects of CSP on flame retardancy, thermal properties, heat release rate (HRR), smoke production, and mechanical properties of EP/CSP composites were investigated by limiting oxygen index (LOI), UL-94 tests, thermogravimetric analysis (TGA), cone calorimeter, and tensile test. LOI and UL-94 indicated that CSP remarkably improved the flame retardancy of EP composites. TGA showed that the initial decomposition temperature and the maximum-rate decomposition temperature decreased, whereas residue yields at high temperature increased with the incorporation of microspheres. Cone calorimetry gave evidence that HRR, peak release rate, average HRR, and smoke production rate of EP/CSP composites decreased significantly. The morphology of char residues suggested that CSP could effectively promote EP to form high-quality char layer with compact outer surface and swollen inner structure. Tensile strength of EP was enhanced with the addition of CSP. V C 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40218.

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“…This finding is in agreement with other studies, which were reported by Yasmin et al (2015) in sheep oocytes, and Terada et al (2002) in several mammalian cell lines in which sericin was used to accelerate cell proliferation. Sericin may serve as an alternative supplement in serum-free media for cell culture (Tunma et al, 2013). Yanagihara et al (2006) reported that sericin is a powerful alternative to the production of the adenoviral vector in human cells.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide concentration and DNA fragmentation of buffalo oocytes matured in sericin-supplemented maturation medium

et al. 2019

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This study was conducted to investigate the effects of sericin on meiotic maturation, hydrogen peroxide (H 2 O 2) concentration and deoxyribonucleic acid (DNA) fragmentation in buffalo oocytes. Oocytes were matured in vitro in tissue culture medium (TCM-199) with in vitro maturation (IVM) groups under several conditions, namely without bovine serum albumin (-BSA), (+BSA), and 0.025%, 0.05%, 0.1% and 0.25% (w/v) sericin. The results showed that supplementation of the maturation medium with 0.05% sericin significantly increased the rate of oocytes that reach metaphase II compared with other groups, except for the 0.025% sericin-treated group. Intracellular H 2 O 2 concentrations in oocytes of all groups were measured using 2',7'-dichlorodihydrofluorescein diacetate (DCHFDA). The concentration of H 2 O 2 in matured oocytes treated with 0.05% sericin was lower than in other groups. DNA fragmentation and the nuclear status of oocytes were examined using the terminal deoxyribonucleotidyl transferase-mediated dUTP digoxigenin nick end-labelling (TUNEL) method. The total proportion of TUNEL-positive oocytes at the MII stage was lower in the 0.05% sericin group. The results indicate that addition of 0.05% sericin to the maturation medium may improve nuclear maturation and may inhibit DNA fragmentation in oocytes by decreasing H 2 O 2 concentrations.

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Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture

Cited by 11 publications

References 22 publications

Silk Sericin-Functionalized Bacterial Cellulose as a Potential Wound-Healing Biomaterial

Silk Sericin-Functionalized Bacterial Cellulose as a Potential Wound-Healing Biomaterial

Flame retardant and mechanical properties of epoxy composites containing APP−PSt core−shell microspheres

Hydrogen peroxide concentration and DNA fragmentation of buffalo oocytes matured in sericin-supplemented maturation medium

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