Immobilization of Rhizopus oryzae lipase on magnetic Fe3O4-chitosan beads and its potential in phenolic acids ester synthesis

Immobilization of feruloyl esterases on magnetic nanoparticles and its potential in production of ferulic acid

“…The activity and specific activity of immobilized enzyme decreased rapidly when pH exceeded 6.0. A possible explanation is that the high pH of the reaction solution changed the structure of the enzyme protein, results in protein deactivation before adsorption (33,34). This result can also be verified in the next experiments, the activity of free FAEs was decreased with the increase of pH (pH > 6).…”

Increase of stability of oleate hydratase by appropriate immobilization technique and conditions

“…Using AMP-CHT (prepared as 17 described in section 2.4.4.4) as support and glutaraldehyde as linker, about 24% (about 18 51U/g) of the initial activity was recovered following the immobilization, which can be 19 considered a good result for such a difficult and unstable enzyme. The higher 20 immobilization efficiency compared to other methods is due to the availability of 21 numerous lysine groups near to the surface for covalent binding (Fig. 2Sb).…”

“…500 µl OHase solution (2.5 mg ml -1 ) was added and the mixture was 5 shaken at 4ºC, 100 rpm, for 24h. The washing step was performed as described in Magnetic chitosan composites containing amino terminated magnetic particles (AMP) 9 dispersed in chitosan macroparticles have been prepared as described elsewhere [20], 10 with some modifications. Chitosan 2% solution in acetic acid (2%) was added to 187.5 11 mg wet AMP, in 5:1 ratio (w/w).…”

“…Adsorption: Immobilization of OHase on Celite 545, a slightly hydrophobic support, 20 resulted in low protein loading and low recovered activity. (Table 1, Table 1, entry 4) exceeded 95%, but the specific activity of 11.8 U/g protein 10 corresponds to only 5.5% recovered activity.…”

Recent progress in nanobiocatalysis for enzyme immobilization and its application

“…Kumar et al conducted the ethyl esterification of ferulic acid in an iso-octane solvent system by using the immobilized lipase and obtained 89.6% of the conversion rate. Furthermore, the immobilized lipase retained 72.6% of its initial activity after 8 reuses, indicating high stability in pharmaceutical and industrial applications [73].…”