Immobilization of feruloyl esterases on magnetic nanoparticles and its potential in production of ferulic acid

He, Fuming; Zhang, Song; Liu, Xinli

doi:10.1016/j.jbiosc.2015.01.006

Cited by 21 publications

(4 citation statements)

References 42 publications

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“…Monocomponent FAEs have been immobilized on magnetic Fe 3 O 4 nanoparticles, leading to an increase in optimal temperature from 45 o C to 55 o C and temperature stability, retaining 52.4% of its initial activity for the release of FA from insoluble wheat bran after 5 cycles (He et al, 2015). Supermagnetic Fe 3 O 4 @Au core-shell nanoparticles were used to immobilize a putative FAE, PhEst, with a 2-fold increase in activity compared to the free enzyme (Parracino et al, 2011).…”

mentioning

confidence: 99%

Feruloyl esterases: Biocatalysts to overcome biomass recalcitrance and for the production of bioactive compounds

Oliveira

Mota

Oliva

et al. 2019

Bioresource Technology

105

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Ferulic acid and its hydroxycinnamate derivatives represent one of the most abundant forms of low molecular weight phenolic compounds in plant biomass. Feruloyl esterases are part of a microorganism's plant cell wall-degrading enzymatic arsenal responsible for cleaving insoluble wall-bound hydroxycinnamates and soluble cytosolic conjugates. Stimulated by industrial requirements, accelerating scientific discoveries and knowledge transfer, continuous improvement efforts have been made to identify, create and repurposed biocatalysts dedicated to plant biomass conversion and biosynthesis of high-added value molecules. Here we review the basic knowledge and recent advances in biotechnological characteristics and the gene content encoding for feruloyl esterases. Information about several enzymes is systematically organized according to their function, biochemical properties, substrate specificity, and biotechnological applications. This review contributes to further structural, functional, and biotechnological R&D both for obtaining hydroxycinnamates from agricultural by-products as well as for lignocellulose biomass treatments aiming for production of bioethanol and other derivatives of industrial interest.

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mentioning

confidence: 99%

Feruloyl esterases: Biocatalysts to overcome biomass recalcitrance and for the production of bioactive compounds

Oliveira

Mota

Oliva

et al. 2019

Bioresource Technology

105

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“…[79] Note that those enzymes are not only produced at the industrial scale, but they also are often supplied in the form of a cocktail, lowering the price and possibly containing secondary activities such as feruloyl esterases. [113] Other advantages of enzymes are their substrate specificity (discrimination between the p-HCAs), [114] recyclability, [115] and no deleterious effect on the substrate that can be reused for further applications (e. g., feed, methanization, ethanol production). Moreover, the use of enzymes can simplify the downstream processing as few degradation products are recovered compared to alkaline hydrolysis (e. g., discoloration step).…”

Section: Discussionmentioning

confidence: 99%

Accessing p‐Hydroxycinnamic Acids: Chemical Synthesis, Biomass Recovery, or Engineered Microbial Production?

et al. 2020

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p-Hydroxycinnamic acids (i. e., p-coumaric, ferulic, sinapic, and caffeic acids) are phenolic compounds involved in the biosynthesis pathway of lignin. These naturally occurring molecules not only exhibit numerous attractive properties, such as antioxidant, anti-UV, and anticancer activities, but they also have been used as building blocks for the synthesis of tailored monomers and functional additives for the food/feed, cosmetic, and plastics sectors. Despite their numerous high value-added applications, the sourcing of p-hydroxycinnamic acids is not ensured at the industrial scale except for ferulic acid, and their production cost remains too high for commodity applications. These compounds can be either chemically synthesized or extracted from lignocellulosic biomass, and recently their production through bioconversion emerged. Herein the different strategies described in the literature to produce these valuable molecules are discussed.

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“…Feruloyl esterases (FAE) were also successfully immobilized on magnetic iron nanoparticles via physical adsorption. Optimal immobilization conditions were significantly influenced by pH 6, where the maximum activity of immobilized FAEs were observed [91]. Because physical adsorption is a simple and mild method, it involves weak interactions, where enzymes are attached to the matrix through hydrogen bonds [92], van der Waals forces [93,94], and hydrophobic interactions [95].…”

Section: Physical Adsorptionmentioning

confidence: 99%

Micro‐ and Nanocarriers for Immobilization of Enzymes

Leitgeb¹,

Knez²,

Vasić³

2016

Micro and Nanotechnologies for Biotechnology

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Two types of micro-and nanocarriers for immobilization of enzymes for biotechnological and biomedical applications are described: magnetic nanoparticles and crosslinked enzyme aggregates (CLEAs). Nanosized structures with their large surface and smaller size volume ratio, which is dependent on their strong magnetic dipole, give key features that make magnetic nanoparticles useful in many biotechnological and biomedical applications. They are therefore used as carriers to which different active substances can bind. The preparation of the magnetic nanoparticles, possible surface coating methods, and functionalization with different materials are described. Enzyme immobilization methods, such as adsorption, affinity binding, chelation, or metal binding or covalent binding, enable the preparation of efficient and stable enzyme bound to magnetic nanoparticles. Such a product may be used among bioreactor applications for targeted drug delivery in biosensors or bioimaging and magnetic resonance imaging. Preparation of CLEAs, the microsized enzyme structures without a carrier, is described as well. Their main advantage is very simple preparation, where two steps, precipitation of the enzyme and cross-linking, are joined. A broad spectrum of enzymes for CLEA preparation has been used and many biotechnological reactions are catalyzed. The improvement in CLEA preparation to enhance their stability and operability is also shown.

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Immobilization of feruloyl esterases on magnetic nanoparticles and its potential in production of ferulic acid

Cited by 21 publications

References 42 publications

Feruloyl esterases: Biocatalysts to overcome biomass recalcitrance and for the production of bioactive compounds

Feruloyl esterases: Biocatalysts to overcome biomass recalcitrance and for the production of bioactive compounds

Accessing p‐Hydroxycinnamic Acids: Chemical Synthesis, Biomass Recovery, or Engineered Microbial Production?

Micro‐ and Nanocarriers for Immobilization of Enzymes

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