Immobilization of recombinant Escherichia coli whole cells harboring xylose reductase and glucose dehydrogenase for xylitol production from xylose mother liquor

Jin, Liqun; Yang, Bo; Wei, Xianfu; Chen, Xian-Xiao; Jia, Dong‐Xu; Liu, Zhi‐Qiang; Zheng, Yu‐Guo

doi:10.1016/j.biortech.2019.121344

Cited by 34 publications

(20 citation statements)

References 34 publications

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“…This acid hydrolysate contains many other sugars and impurities that complicate xylose purification and lead to the production of xylose mother liquor (XML) as the major by-product of xylose manufacture [ 3 , 4 ]. XML is a dark brown, nutrient-dense liquor that contains xylose (35–40%), glucose (8–10%), l -arabinose (10–15%), d -galactose (8–10%), and trace amount of other sugars [ 1 , 5 , 6 ]. The production of one ton of xylose results in the formation of one to one and a half tons of XML.…”

Section: Introductionmentioning

confidence: 99%

“…Use of this strain increased the l- arabinose content of the XML to 86.1% of total sugars, allowing the l -arabinose to be isolated using a simple process. Other microbial processes have been used to convert XML to high-value chemicals such as xylitol [ 1 , 6 ], solvents [ 3 , 9 ], welan gum [ 10 ] and succinic acid [ 7 ]. However, XML also contains furfural and 5-hydroxymethyl furfural, which are potent inhibitors of microbial growth.…”

Section: Introductionmentioning

confidence: 99%

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Stepwise metabolic engineering of Candida tropicalis for efficient xylitol production from xylose mother liquor

et al. 2021

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Background Commercial xylose purification produces xylose mother liquor (XML) as a major byproduct, which has become an inexpensive and abundant carbon source. A portion of this XML has been used to produce low-value-added products such as caramel but the remainder often ends up as an organic pollutant. This has become an issue of industrial concern. In this study, a uracil-deficient Candida tropicalis strain was engineered to efficiently convert XML to the commercially useful product xylitol. Results The xylitol dehydrogenase gene was deleted to block the conversion of xylitol to xylulose. Then, an NADPH regeneration system was added through heterologous expression of the Yarrowia lipolytica genes encoding 6-phosphate-gluconic acid dehydrogenase and 6-phosphate-glucose dehydrogenase. After process optimization, the engineered strain, C. tropicalis XZX-B4ZG, produced 97.10 g L− 1 xylitol in 120 h from 300 g L− 1 XML in a 5-L fermenter. The xylitol production rate was 0.82 g L− 1 h− 1 and the conversion rate was 92.40 %. Conclusions In conclusion, this study performed a combination of metabolic engineering and process optimizing in C. tropicalis to enhance xylitol production from XML. The use of C. tropicalis XZX-B4ZG, therefore, provided a convenient method to transform the industrial by-product XML into the useful material xylitol.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stepwise metabolic engineering of Candida tropicalis for efficient xylitol production from xylose mother liquor

et al. 2021

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“…Adsorption is based on secondary interactions, such as ionic interactions [91]. To diminish cell leakage, adsorption as first step can be combined with other immobilization techniques like encapsulation, coatings or covalent cross-linking after flocculation [92,93]. In this case the adsorbents and the flocculants have great impact on the produced biocatalyst.…”

Section: Introductionmentioning

confidence: 99%

“…In this case the adsorbents and the flocculants have great impact on the produced biocatalyst. The addition of solid support, such as diatomite, bentonite, silica microspheres, could increase mechanical and chemical stability and the biocatalytic activity compared to the cross-linked or entrapped cells without solid support [32,92]. For cross-linking and covalent immobilization onto solid surfaces, glutaraldehyde is widely used [92,94].…”

Section: Introductionmentioning

confidence: 99%

“…The addition of solid support, such as diatomite, bentonite, silica microspheres, could increase mechanical and chemical stability and the biocatalytic activity compared to the cross-linked or entrapped cells without solid support [32,92]. For cross-linking and covalent immobilization onto solid surfaces, glutaraldehyde is widely used [92,94]. It is getting popular to combine these methods and merge the advantageous properties of the different systems creating new hybrid matrices for whole cell or enzyme immobilization [95,96].…”

Section: Introductionmentioning

confidence: 99%

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“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media

et al. 2019

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This article overviews the numerous immobilization methods available for various biocatalysts such as whole-cells, cell fragments, lysates or enzymes which do not require preliminary enzyme purification and introduces an advanced approach avoiding the costly and time consuming downstream processes required by immobilization of purified enzyme-based biocatalysts (such as enzyme purification by chromatographic methods and dialysis). Our approach is based on silica shell coated magnetic nanoparticles as solid carriers decorated with mixed functions having either coordinative binding ability (a metal ion complexed by a chelator anchored to the surface) or covalent bond-forming ability (an epoxide attached to the surface via a proper linker) enabling a single operation enrichment and immobilization of a recombinant phenylalanine ammonia-lyase from parsley fused to a polyhistidine affinity tag.

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Immobilization of Microbial Consortium for Glutaric Acid Production from Lysine

et al. 2021

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Glutaric acid is an important C5 platform compound for the synthesis of nylon polymers. However, its production titer and cost are limited by the need for expensive cofactors addition as well as the difficulty of reusing microbial catalysts. Herein, to address these problems, two regeneration systems each for NAD(P) + and α-ketoglutaric acid were introduced into a fiveenzyme cadaverine pathway for a whole-cell biocatalytic approach. We demonstrated the efficient conversion of lysine to glutaric acid in a synthetic microbial consortium manner, producing 73.2 g/L glutaric acid with a 74 % molar yield in a 5-L bioreactor scale. Moreover, with a colloidal chitin-based immobilization, the engineered microbial consortium exhibited good stability and reusability in a five-cycle bioconversion. This study provides an efficient microbial platform for glutaric acid production by developing a cheaper and universal cell immobilization approach.

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Immobilization of recombinant Escherichia coli whole cells harboring xylose reductase and glucose dehydrogenase for xylitol production from xylose mother liquor

Cited by 34 publications

References 34 publications

Stepwise metabolic engineering of Candida tropicalis for efficient xylitol production from xylose mother liquor

Stepwise metabolic engineering of Candida tropicalis for efficient xylitol production from xylose mother liquor

“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media

Immobilization of Microbial Consortium for Glutaric Acid Production from Lysine

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