2005
DOI: 10.1016/j.bej.2005.03.007
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Immobilization of Pycnoporus sanguineus laccase on magnetic chitosan microspheres

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Cited by 373 publications
(160 citation statements)
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“…The support beads were activated using glutaraldehyde and immobilization was performed by direct contact of the enzyme solution with the activated chitosan/clay beads [39]. The percentage of glutaraldehyde solution used to activate the beads is very important as it promotes modification in the spheres geometrical parameters, and is also associated with structural modifications in the enzyme after its activation.…”
Section: Enzyme Immobilizationmentioning
confidence: 99%
“…The support beads were activated using glutaraldehyde and immobilization was performed by direct contact of the enzyme solution with the activated chitosan/clay beads [39]. The percentage of glutaraldehyde solution used to activate the beads is very important as it promotes modification in the spheres geometrical parameters, and is also associated with structural modifications in the enzyme after its activation.…”
Section: Enzyme Immobilizationmentioning
confidence: 99%
“…In the FTIR spectra of magnetic chitosan microspheres obtained by De-Sheng J et al the (C), the peak was at 3427 cm −1 corresponds to stretching vibrations of hydroxyl. The C-H stretching vibration of the polymer backbone is manifested through strong peak at 2925 cm −1 and 2855 cm −1 [30]. The stretch vibrations of C-O are found at 1084 cm −1 and 1032 cm −1 .…”
Section: Results and Discusssionmentioning
confidence: 92%
“…Various sustained release drug carriers have been made from chitosan such as microparticles [15], tablets [16], gel [17] and beads [18]. Chitosan microspheres have been widely investigated for use as controlled release delivery systems for hormones [19], vitamins [20], proteins [21] and enzymes [22].…”
Section: Introductionmentioning
confidence: 99%
“…1, spectrum d , the IR bands responsible for the lipase that was chemically covalent-bonded to the magnetic particles, were observed at 1636 cm 1 for amide and at 1549 cm 1 for amide 27 . In addition, the IR peak appeared at 1662 cm 1 could be assignable to stretching vibrations of C N bond, indicating the formation of schiff base as a result of the reaction between carbonyl group of glutaraldehyde and amine group of lipase 28 . ites, the same characteristic XRD peaks were also observed.…”
Section: Characteristics Of the Immobilized Lipasementioning
confidence: 97%