Immobilization of Pseudomonas cepacia lipase onto electrospun polyacrylonitrile fibers through physical adsorption and application to transesterification in nonaqueous solvent

Sakai, Shinji; Liu, Yuping; Yamaguchi, Tsuyoshi; Watanabe, Rie; Kawabe, Mayumi; Kawakami, Koei

doi:10.1007/s10529-010-0279-8

Cited by 58 publications

(18 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cross-linking of enzymes to electrospun nanofibers has shown greater residual activity due to increased surface area and porosity. Use of such nanodiametric supports have brought a turning point in the field of biocatalyst immobilization (Wu et al 2005; Kim et al 2006; Ren et al 2006; Li et al 2007; Huang et al 2008; Sakai et al 2010). Covalent binding of alcohol dehydrogenase on attapulgite nanofibers (hydrated magnesium silicate) has been opted owing to its thermal endurance and variable nano sizes (Zhao et al 2010).…”

Section: Different Techniques Used For Immobilizationmentioning

confidence: 99%

Enzyme immobilization: an overview on techniques and support materials

2012

View full text Add to dashboard Cite

The current demands of the world’s biotechnological industries are enhancement in enzyme productivity and development of novel techniques for increasing their shelf life. These requirements are inevitable to facilitate large-scale and economic formulation. Enzyme immobilization provides an excellent base for increasing availability of enzyme to the substrate with greater turnover over a considerable period of time. Several natural and synthetic supports have been assessed for their efficiency for enzyme immobilization. Nowadays, immobilized enzymes are preferred over their free counterpart due to their prolonged availability that curtails redundant downstream and purification processes. Future investigations should endeavor at adopting logistic and sensible entrapment techniques along with innovatively modified supports to improve the state of enzyme immobilization and provide new perspectives to the industrial sector.

show abstract

Section: Different Techniques Used For Immobilizationmentioning

confidence: 99%

Enzyme immobilization: an overview on techniques and support materials

2012

View full text Add to dashboard Cite

show abstract

“…A wide range of bioactive compounds can be decorated on the surface of electrospun fibers by physical adsorption. Sakai et al (2010) immobilized Pseudomonas cepacia lipase onto the electrospun polyacrylonitrile fibers by physical adsorption. The immobilized lipase showed more than 20-fold higher activity than the free lipase, and the initial reaction rate after 10 recycles was 80% of that in the first run, indicating that the recovery and reuse of the enzyme were achieved by the lipaseimmobilizing fibers.…”

Section: Surface Absorptionmentioning

confidence: 99%

Electrospinning of nanofibers: Potentials and perspectives for active food packaging

Zhang

Wang

et al. 2020

Comp Rev Food Sci Food Safe

268

128

View full text Add to dashboard Cite

Electrospun nanofibers with structural and functional advantages have drawn much attention due to their potential applications for active food packaging. The traditional role of food packaging is just storage containers for food products. The changes of retailing practice and consumer demand promote the development of active packaging to improve the safety, quality, and shelf life of the packaged foods. To develop the technique of electrospinning for active food packaging, electrospun nanofibers have been covalently or non‐covalently functionalized for loading diverse bioactive compounds including antimicrobial agents, antioxidant agents, oxygen scavengers, carbon dioxide emitters, and ethylene scavengers. The aim of this review is to present a concise but comprehensive summary on the progress of electrospinning techniques for active food packaging. Emphasis is placed on the tunability of the electrospinning technique, which achieves the modification of fiber composition, orientation, and architecture. Efforts are also made to provide functionalized strategies of electrospun polymeric nanofibers for food packaging application. Furthermore, the existing limitations and prospects for developing electrospinning in food packaging area are discussed.

show abstract

“…Sudwilai prepared PPy‐coated electrospun poly(lactic acid) nanofibers with low surface conductivity. These studies demonstrated that a conductive polymer coating can be used in place of a filler to enhance the conductivity . However, two problems remain for the electric conductivity improvement of electrospun fibers: (1) small conductive particles tend to aggregate, especially at the lap joints of fibers and (2) the Van der Waals forces that bind conductive fillers and electrospun fibers are weak, which has a negative effect with the long‐term usage.…”

Section: Introductionmentioning

confidence: 99%

Improvements on electrical conductivity of the electrospun microfibers using the silver nanoparticles

Wei

Qiu

Wang

et al. 2019

J of Applied Polymer Sci

View full text Add to dashboard Cite

Conductive electrospun polymer fibers have attracted a great deal of interest in recent years. This study describes the preparation of electrically conductive microfibers composed of polyethersulfone/polydopamine/silver nanoparticles (PES/PDA/Ag NPs). Ag NPs acted as conductive centers, while hydroxyl‐ and amino‐rich functional groups and excellent adhesion properties of PDA served to connect the Ag NPs and PES microfibers. Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) showed that PDA was firmly adhered to PES microfibers. PES/PDA microfibers absorbed considerable amounts of silver ion from AgNO3 solution, resulting in Ag NPs. X‐ray diffraction, X‐ray photoelectron spectroscopy, and SEM data represented the successful formation of PES/PDA/Ag NPs microfibers. Microfibers with optimal conductivity were obtained using a solution of 2% AgNO3 at pH 9 at 50 °C for 45 min. The electrical resistivity of our PES/PDA/Ag NPs microfibers was only 202 Ω/cm, much lower than that of regular PES microfibers (2.1 × 109 Ω/cm). These results show that the PES/PDA/Ag NPs microfibers are suitable for use as conductive polymer fibers in electromagnetic shielding, and conductivity‐sensing applications, and in flexible electronic devices and biosensors. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48788.

show abstract

Immobilization of Pseudomonas cepacia lipase onto electrospun polyacrylonitrile fibers through physical adsorption and application to transesterification in nonaqueous solvent

Cited by 58 publications

References 11 publications

Enzyme immobilization: an overview on techniques and support materials

Enzyme immobilization: an overview on techniques and support materials

Electrospinning of nanofibers: Potentials and perspectives for active food packaging

Improvements on electrical conductivity of the electrospun microfibers using the silver nanoparticles

Contact Info

Product

Resources

About