Immobilization of Octadecyl Ammonium Chloride on the Surface of Titanium and Its Effect on Microbial Colonization In Vitro

Nikawa, Hiroki; Ishida, Kazuhiro; Hamada, Takashi; Satoda, Takahiro; Murayama, Tetsuya; Takemoto, Tsuyoshi; Tamamoto, Mistuhiro; Tajima, Hideaki; Shimoe, Saiji; Fujimoto, Hitomi; Makihira, Seicho

doi:10.4012/dmj.24.570

Cited by 26 publications

(14 citation statements)

References 26 publications

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“…This result is similar to a previous study showing significantly reduction of C. albicans adhesion on SiQAC-coated titanium. In that study, the authors suggested that the octadecyl alkyl chain of the SiQAC molecule has the potential to inhibit initial adhesion of C. albicans [36]. …”

Section: Discussionmentioning

confidence: 99%

Effect of water-aging on the antimicrobial activities of an ORMOSIL-containing orthodontic acrylic resin

Shuili¹,

Epasinghe²,

Zhou³

et al. 2013

Acta Biomaterialia

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Quaternary ammonium methacryloxy silicate (QAMS), an organically modified silicate (ORMOSIL) functionalized with polymerizable methacrylate groups and an antimicrobial agent with a long lipophilic alkyl chain quaternary ammonium group, was synthesized through a silane-based sol–gel route. By dissolving QAMS in methyl methacrylate monomer, this ORMOSIL molecule was incorporated into an auto-polymerizing, powder/liquid orthodontic acrylic resin system, yielding QAMS-containing poly (methyl methacrylate). The QAMS-containing acrylic resin showed a predominant contact-killing effect on Streptococcus mutans (ATCC 35668) and Actinomyces naeslundii (ATCC 12104) biofilms, while inhibiting adhesion of Candida albicans (ATCC 90028) on the acrylic surface. The antimicrobial activities of QAMS-containing acrylic resin were maintained after a 3 month water-aging period. Bromophenol blue assay showed minimal leaching of quaternary ammonium species when an appropriate amount of QAMS (<4 wt.%) was incorporated into the acrylic resin. The results suggest that QAMS is predominantly co-polymerized with the poly(methyl methacrylate) network, and only a minuscule amount of free QAMS molecules is present within the polymer network after water-aging. Acrylic resin with persistent antimicrobial activities represents a promising method for preventing bacteria- and fungus-induced stomatitis, an infectious disease commonly associated with the wearing of removable orthodontic appliances.

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Section: Discussionmentioning

confidence: 99%

Effect of water-aging on the antimicrobial activities of an ORMOSIL-containing orthodontic acrylic resin

Shuili¹,

Epasinghe²,

Zhou³

et al. 2013

Acta Biomaterialia

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“…An ATP assay was used to determine the number of viable microorganisms. To confirm the correlation between number of colonies and ATP content, prior to the experiment, we determined whether ATP luminescence reflected viable cell count (16, 19).…”

Section: Discussionmentioning

confidence: 99%

“…Prevention of microbial adhesion to materials is effective in inhibiting the formation of biofilm. Improvement of the surface conditions of denture base materials was effective in reducing microbial adhesion (15, 16).…”

Section: Introductionmentioning

confidence: 99%

Inhibiting microbial adhesion to denture base acrylic resin by titanium dioxide coating

Arai

Ueda

Sugiyama

et al. 2009

J of Oral Rehabilitation

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Mechanical cleaning of dentures is effective in preventing infections such as aspiration pneumonia and denture stomatitis. For denture wearers with a physical handicap and the elderly, however, mechanical cleaning can present problems. The aim of this study was to investigate the effect of coating denture base acrylic resin with titanium dioxide (TiO(2)) in the inhibition of oral microbial adhesion. We prepared uniformly sized acrylic resin plates (10 mm x 10 mm x 0.5 mm), which were divided into two groups (a non-coated group and a TiO(2)-coated group). The plates were immersed in cultured Streptococcus sanguinis or Candida albicans and incubated for 24 h. After incubation, each plate was washed to remove loosely adherent microorganisms, and then incubated for a further 24 h. Adenosine triphosphate (ATP) content of the microorganisms was evaluated using a reagent containing benzalkonium, which extracts intra-cellular ATP. In addition, to determine biofilm formation, we also observed each plate by scanning electron microscopy (SEM). We found that the ATP content of both S. sanguinis and C. albicans was reduced by the TiO(2) coating (P = 0.000). Observation by SEM confirmed that the TiO(2) coating inhibited biofilm formation. The results indicate that a TiO(2) coating on a denture base acrylic resin inhibits adhesion of S. sanguinis and C. albicans.

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“…Irreversible immobilization of the antimicrobial agents in the device offers an alternative motif that eliminates patient exposure to elutable active agents and potentially increases the duration of antimicrobial efficacy. 8,9 A wide range of antimicrobial agents have been immobilized, including small molecules (e.g., quaternary ammonium silanes), [10][11][12][13][14][15][16][17] quaternary ammonium polymers, [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] polyamines, [36][37][38][39][40][41][42] chitosan, [43][44][45][46][47][48][49] enzymes, [50][51][52][53][54][55] peptides, and peptide mimetics. …”

Section: Introductionmentioning

confidence: 99%

A review of immobilized antimicrobial agents and methods for testing

2011

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Antimicrobial surfaces for food and medical applications have historically involved antimicrobial coatings that elute biocides for effective kill in solution or at surfaces. However, recent efforts have focused on immobilized antimicrobial agents (iAMA) to avoid toxicity, compatibility and reservoir limitations common to elutable agents. This review critically examines the assorted AMAs reported to have been immobilized with an emphasis around interpretation of antimicrobial testing as it pertains to discriminating between eluting and immobilized agents. Immobilization techniques and modes of antimicrobial action are also discussed.

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Immobilization of Octadecyl Ammonium Chloride on the Surface of Titanium and Its Effect on Microbial Colonization In Vitro

Cited by 26 publications

References 26 publications

Effect of water-aging on the antimicrobial activities of an ORMOSIL-containing orthodontic acrylic resin

Effect of water-aging on the antimicrobial activities of an ORMOSIL-containing orthodontic acrylic resin

Inhibiting microbial adhesion to denture base acrylic resin by titanium dioxide coating

A review of immobilized antimicrobial agents and methods for testing

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