Immobilization of naringin onto chitosan substrates by using ozone activation

Li, Chung Hsing; Wang, Jing Wei; Ho, Ming Hua; Shih, Jia Lin; Hsiao, Sheng Wen; Thiện, Đoàn Văn Hồng

doi:10.1016/j.colsurfb.2013.11.006

Cited by 9 publications

(5 citation statements)

References 22 publications

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“…Moreover, we demonstrated that the release of naringin was beneficial to the attachment (Figure ), osteogenesis (Figure ), and mineralization (Figure ) of MSCs. Though the biological activities of naringin have been confirmed, − the mechanism of its osteo-conductivity is complicated and yet to be illuminated. Several studies manifested that extracellular regulated protein kinases (ERK) 1/2 were found to be activated by naringin, and osteogenic differentiation was repressed when the inhibitor of ERK 1/2 was used. , The activation of ERK 1/2 is downstream of the Ras family .…”

Section: Discussionmentioning

confidence: 99%

Controlled Release of Naringin in GelMA-Incorporated Rutile Nanorod Films to Regulate Osteogenic Differentiation of Mesenchymal Stem Cells

et al. 2019

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Naringin, a Chinese herbal medicine, has been demonstrated to concentration-dependently promote osteogenic differentiation of mesenchymal stem cells (MSCs). However, it remains a challenge to load naringin on coatings for osteogenesis and further control the release kinetics. Here, we demonstrated that the release behavior of naringin on rutile nanorod films could be controlled by either mixing naringin with gelatin methacryloyl (GelMA) before spinning onto the films or soaking the obtained GelMA-incorporated films with the naringin solution to achieve the distinct degradation-type release and diffusion-type release, respectively. We further revealed that the naringin-loaded coatings facilitated adhesion, proliferation and late differentiation, and mineralization of MSCs. Our findings provided a novel strategy to engineer the coatings with controlled release of naringin and emphasized the bioactivity of naringin for the osteogenic differentiation of MSCs.

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Section: Discussionmentioning

confidence: 99%

Controlled Release of Naringin in GelMA-Incorporated Rutile Nanorod Films to Regulate Osteogenic Differentiation of Mesenchymal Stem Cells

et al. 2019

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“…The results show that more new bone formation and mineralization was found in the center and periphery of the bone defect area after filling with icariin-loaded bioactive scaffold, which attribute to the upregulating of osteogenic-related factors, such as RUNX2, ALP, OCN, COLI, BSP and OPN [ 76 , 86 , 91 – 94 ]. Besides, other TCM combined with different scaffold materials have similar effects, for instance, icaritin [ 88 , 95 ], hydroxy safflower yellow A [ 96 ], kaempferol [ 97 , 98 ], naringin [ 99 , 100 ], quercetin [ 101 – 103 ], silymarin [ 104 , 105 ], berberine [ 58 , 90 , 106 ], ginsenoside [ 62 , 107 ], resveratrol [ 108 – 110 ], curcumin [ 111 ], and epigallocatechin gallate [ 112 – 115 ]. The rat calvarial defect almost completely repaired with physiological integrity at 16 weeks in the PLGA scaffold incorporated with gelatin, alendronate, and naringin groups, and this might owing to the inhibitory impact of alendronate on osteoclasts and the positive effect of naringin on osteoblasts, the PLGA + Gelatin/ALD/NG scaffold had a high potential for bone repair, as shown by upregulating BMP-2, OSX, OPN, BSP, COLI, OCN and calcium content, and inhibiting TRAP [ 116 ].…”

Section: Btementioning

confidence: 99%

Traditional Chinese medicine promotes bone regeneration in bone tissue engineering

et al. 2022

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Bone tissue engineering (BTE) is a promising method for the repair of difficult-to-heal bone tissue damage by providing three-dimensional structures for cell attachment, proliferation, and differentiation. Traditional Chinese medicine (TCM) has been introduced as an effective global medical program by the World Health Organization, comprising intricate components, and promoting bone regeneration by regulating multiple mechanisms and targets. This study outlines the potential therapeutic capabilities of TCM combined with BTE in bone regeneration. The effective active components promoting bone regeneration can be generally divided into flavonoids, alkaloids, glycosides, terpenoids, and polyphenols, among others. The chemical structures of the monomers, their sources, efficacy, and mechanisms are described. We summarize the use of compounds and medicinal parts of TCM to stimulate bone regeneration. Finally, the limitations and prospects of applying TCM in BTE are introduced, providing a direction for further development of novel and potential TCM. Graphical Abstract

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“…26 Acetic acid (0.5% w v 21 ) was used as the solvent to prepare the chitosan solution of 10% w v 21 . After chitosan solution was casted and dried, chitosan substrates were obtained, followed by the immersion in TPP solution, the crosslinking agent, at 4°C for 2 h, followed by the immersion in saturated Ca(OH) 2 solution for a period of 24 h. Chitosan substrates were then treated with 1.59 SBF prepared by dissolving NaCl (213 mM), NaHCO 3 (6.3 mM), Na 2 SO 4 (0.8 mM), Na 2 HPO 4 (1.5 mM), MgCl 2 AE6H 2 O (2.3 mM), KCl (7.5 mM), and CaCl 2 (3.8 mM) in deionized water, which was described previously.…”

Section: Preparation and Characterization Of Chitosanmentioning

confidence: 99%

“…The osteoconductivity of scaffolds is critical in bone regeneration, because scaffolds influence not only cell proliferation but also osteogenic differentiation. According to previous researches, the osteoconductivity of biomaterials is affected by materials' chemical compositions, 26 hydrophilicity, 20 mechanical strength, 32,36,50 roughness, 13 crystallinity, 18 etc. Information related to materials' osteoconductivity is possibly provided by a serious of in vivo or in vitro experiments.…”

Section: Introductionmentioning

confidence: 98%

Preparation of Chitosan/Hydroxyapatite Substrates with Controllable Osteoconductivity Tracked by AFM

Hsiao

et al. 2014

Ann Biomed Eng

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In this study, cell-material adhesive strength and cellular mechanical properties were measured using atomic force microscopy (AFM) to track cell attachment and osteogenic differentiation. First, chitosan substrates were treated with simulated body fluid (SBF) for various periods, resulting in substrates with different osteoconductivity. The X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS) and in vitro tests revealed that the biomimeticity and osteoconductivity of substrates increased with increasing time of SBF treatment. When the SBF immersion exceeded 14 days, the chitosan substrates exhibited their highest biocompatibility and osteoconductivity. AFM measurements indicated specifically high adhesive forces between SBF-treated chitosan and osteogenic cells, causing better cell attachment. The results demonstrate that cell adhesion was controlled by cell-material adhesive strength, which were in turn controlled via the SBF treatment time. The adhesive strength between cells and material also accounted for the chitosan substrates' specific selectivity toward osteogenic cells. A two-step increase in mechanical strength was observed for the nucleus and cytoplasm of osteogenic cells. The results indicate that through the use of AFM, the real-time cell-material interforce and cellular mechanics can be identified. The adhesive strength was positively correlated to the cell attachment, and the second increase in the Young's modulus of nucleus and cytoplasm was correlated to early osteogenic differentiation.

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Immobilization of naringin onto chitosan substrates by using ozone activation

Cited by 9 publications

References 22 publications

Controlled Release of Naringin in GelMA-Incorporated Rutile Nanorod Films to Regulate Osteogenic Differentiation of Mesenchymal Stem Cells

Controlled Release of Naringin in GelMA-Incorporated Rutile Nanorod Films to Regulate Osteogenic Differentiation of Mesenchymal Stem Cells

Traditional Chinese medicine promotes bone regeneration in bone tissue engineering

Preparation of Chitosan/Hydroxyapatite Substrates with Controllable Osteoconductivity Tracked by AFM

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