Immobilization of methotrexate anticancer drug onto the graphene surface and interaction with calf thymus DNA and 4T1 cancer cells

Shervedani, Reza Karimi; Mirhosseini, Hadiseh; Foroushani, Marzieh Samiei; Torabi, Mostafa; Rahsepar, Fatemeh Rahnemaye; Norouzi-Barough, Leila

doi:10.1016/j.bioelechem.2017.08.004

Cited by 25 publications

(7 citation statements)

References 48 publications

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“…Alginate conjugated GO-DOX did not produce any cell cytotoxicity by MTT assay on NIH-3T3 and HeLa cell lines. Methotrexate (an anticancer drug) was immobilized on the graphene surface to evaluate the drug-carrying capacity of graphene nanomaterials (Karimi Shervedani et al, 2018). A study on 4T1 cancer cell lines demonstrated successful carrier capacity and cellular capacity through the electrochemical method.…”

Section: Graphenementioning

confidence: 99%

Drug Delivery With Carbon-Based Nanomaterials as Versatile Nanocarriers: Progress and Prospects

Debnath

Srivastava

2021

Front. Nanotechnol.

114

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With growing interest, a large number of researches have been conducted on carbon-based nanomaterials (CBNs). However, their uses are limited due to comprehensive potential environmental and human health effects. It is often confusing for researchers to make an informed choice regarding the versatile carbon-based nanocarrier system and its potential applications. This review has highlighted emerging applications and cutting-edge progress of CBNs in drug delivery. Some critical factors like enzymatic degradation, surface modification, biological interactions, and bio-corona have been discussed here. These factors will help to fabricate CBNs for effective drug delivery. This review also addresses recent advancements in carbon-based target specific and release controlled drug delivery to improve disease treatment. The scientific community has turned their research efforts into the development of novel production methods of CBNs to make their production more attractive to the industrial sector. Due to the nanosize and diversified physical properties, these CBNs have demonstrated distinct biological interaction. Thus long-term preclinical toxicity study is recommended before finally translating to clinical application.

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Section: Graphenementioning

confidence: 99%

Drug Delivery With Carbon-Based Nanomaterials as Versatile Nanocarriers: Progress and Prospects

Debnath

Srivastava

2021

Front. Nanotechnol.

114

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“…The SN38-PEG-GO was water soluble and stable in phosphate buffer saline and mouse serum and showed enhanced cell killing ability compared with a water-soluble drug CPT-11. This pioneer study has stimulated the reach on the delivery of various drugs, e.g., doxorubicin hydrochloride (DOX) [74][75][76][77][78], chlorin e6 (Ce6) [79,80], camptothecin (CPT) [81,82], methotrexate (MTX) [83][84][85], and ibuprofen [86]. As the most effective drug for cancer treatment, DOX is widely investigated.…”

Section: Graphenementioning

confidence: 99%

Functionalization of Carbon Nanomaterials for Biomedical Applications

Liu

Speranza

2019

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Over the past decade, carbon nanostructures (CNSs) have been widely used in a variety of biomedical applications. Examples are the use of CNSs for drug and protein delivery or in tools to locally dispense nucleic acids to fight tumor affections. CNSs were successfully utilized in diagnostics and in noninvasive and highly sensitive imaging devices thanks to their optical properties in the near infrared region. However, biomedical applications require a complete biocompatibility to avoid adverse reactions of the immune system and CNSs potentials for biodegradability. Water is one of the main constituents of the living matter. Unfortunately, one of the disadvantages of CNSs is their poor solubility. Surface functionalization of CNSs is commonly utilized as an efficient solution to both tune the surface wettability of CNSs and impart biocompatible properties. Grafting functional groups onto the CNSs surface consists in bonding the desired chemical species on the carbon nanoparticles via wet or dry processes leading to the formation of a stable interaction. This latter may be of different nature as the van Der Waals, the electrostatic or the covalent, the π-π interaction, the hydrogen bond etc. depending on the process and on the functional molecule at play. Grafting is utilized for multiple purposes including bonding mimetic agents such as polyethylene glycol, drug/protein adsorption, attaching nanostructures to increase the CNSs opacity to selected wavelengths or provide magnetic properties. This makes the CNSs a very versatile tool for a broad selection of applications as medicinal biochips, new high-performance platforms for magnetic resonance (MR), photothermal therapy, molecular imaging, tissue engineering, and neuroscience. The scope of this work is to highlight up-to-date using of the functionalized carbon materials such as graphene, carbon fibers, carbon nanotubes, fullerene and nanodiamonds in biomedical applications.

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“…These nanoparticles provide the researchers with more efficient techniques for interfacing DNA recognition with electrochemical signal transduction. The recent electrochemical drug‐DNA binding studies based on nanomaterials are summarized in Table ; the types of drugs that have been analyzed are anticancer agents antibiotics, antihistaminic, antivirals, nonsteroidal anti‐inflammatory drugs, neurotransmitters, vitamins and retroviral . Upon discovery of the advantages that nanomaterials could bring to analytical science, the first researches regarding to DNA‐drug interaction were done in solution.…”

Section: Nanomaterialsmentioning

confidence: 99%

“…In 2018, Shervedani et al. published an interesting paper as a prove concept, where they reported the preparation of a pharmacosensor by immobilizing methotrexate (MTX) anticancer drug in three different ways: a) covalent bonding using EDC/NHS as the organic activators to form the amide bond, b) electrografting of MTX diazonium salt and c) noncovalent bonding, being electrografting the best method to immobilize the drug. To evaluate the interaction ability of the immobilized MTX with biological species they used calf thymus DNA (ctDNA), mouse 4T1 breast tumor and Human foreskin fibroblast (hFF) cells as models of the primary intracellular target of anticancer drugs, for cancer and normal cells, respectively.…”

Section: Nanomaterialsmentioning

confidence: 99%

Nanomaterial‐based Sensors for the Study of DNA Interaction with Drugs

2019

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The interaction of drugs with DNA has been searched thoroughly giving rise to an endless number of findings of undoubted importance, such as a prompt alert to harmful substances, ability to explain most of the biological mechanisms, or provision of important clues in targeted development of novel chemotherapeutics. The existence of some drugs that induce oxidative damage is an increasing point of concern as they can cause cellular death, aging, and are closely related to the development of many diseases. Because of a direct correlation between the response, strength/ nature of the interaction and the pharmaceutical action of DNA‐targeted drugs, the electrochemical analysis is based on the signals of DNA before and after the interaction with the DNA‐targeted drug. Nowadays, nanoscale materials are used extensively for offering fascinating characteristics that can be used in designing new strategies for drug‐DNA interaction detection. This work presents a review of nanomaterials (NMs) for the study of drug‐nucleic acid interaction. We summarize types of drug‐DNA interactions, electroanalytical techniques for evidencing these interactions and quantification of drug and/or DNA monitoring.

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Immobilization of methotrexate anticancer drug onto the graphene surface and interaction with calf thymus DNA and 4T1 cancer cells

Cited by 25 publications

References 48 publications

Drug Delivery With Carbon-Based Nanomaterials as Versatile Nanocarriers: Progress and Prospects

Drug Delivery With Carbon-Based Nanomaterials as Versatile Nanocarriers: Progress and Prospects

Functionalization of Carbon Nanomaterials for Biomedical Applications

Nanomaterial‐based Sensors for the Study of DNA Interaction with Drugs

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