Immobilization of mannanase on sodium alginate-grafted-β-cyclodextrin: An easy and cost effective approach for the improvement of enzyme properties

Dhiman, Shikha; Srivastava, Binti; Singh, Gursharan; Khatri, Madhu; Arya, Shailendra Kumar

doi:10.1016/j.ijbiomac.2019.11.175

Cited by 49 publications

(27 citation statements)

References 31 publications

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“…Dincer et al [ 34 ] observed 67% immobilization yield when tyrosinase was immobilized on chitosan-clay composite beads. Dhiman et al [ 12 ] immobilized β-mannanase on sodium alginate-grafted-β-cyclodextrin, which resulted in 91.5% immobilization yield. This suggests that the enzyme leakage in our study was higher compared to study by Dhiman et al [ 12 ], which could be improved in the future studies.…”

Section: Resultsmentioning

confidence: 99%

“…Dhiman et al [ 12 ] immobilized β-mannanase on sodium alginate-grafted-β-cyclodextrin, which resulted in 91.5% immobilization yield. This suggests that the enzyme leakage in our study was higher compared to study by Dhiman et al [ 12 ], which could be improved in the future studies. The protein loading efficiency of Man/Cel5B was found to be 73.3% which was almost similar to the protein loading efficiency (78.4%) observed by Kaushal et al [ 23 ] when catalase was immobilized on glutaraldehyde cross-linked chitosan beads.…”

Section: Resultsmentioning

confidence: 99%

“…In addition to this, immobilization enables easy recovery of biocatalysts from the end product and provides opportunities for scaling up and enabling the development of processes based on different reactor configurations and reduction of operating costs [ 11 ]. Previously, β-mannanases have been immobilized on different support materials which include sodium alginate-grafted β-cyclodextrin [ 12 ], chitosan magnetic nanocomposites [ 13 ], cross-linked enzyme aggregates [ 14 ], and calcium alginate beads [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Modifying Thermostability and Reusability of Hyperthermophilic Mannanase by Immobilization on Glutaraldehyde Cross-Linked Chitosan Beads

et al. 2022

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In the current study, the purified β-mannanase (Man/Cel5B) from Thermotoga maritima was immobilized on glutaraldehyde cross-linked chitosan beads. The immobilization of Man/Cel5B on chitosan beads was confirmed by Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis. After immobilization, the protein loading efficiency and immobilization yield were found to be 73.3% and 71.8%, respectively. The optimum pH for both free and immobilized enzymes was found to be pH 5.5. However, the optimum temperature of immobilized Man/Cel5B increased by 10 °C, from 85 °C (free Man/Cel5B) to 95 °C (Immobilized). The half-life of free and immobilized enzymes was found to be 7 h and 9 h, respectively, at 85 °C owing to the higher thermostability of immobilized Man/Cel5B. The increase in thermostability was also demonstrated by an increase in the energy of deactivation (209 kJmol−1) for immobilized enzyme compared to its native form (92 kJmol−1), at 85 °C. Furthermore, the immobilized Man/Cel5B displayed good operational stability as it retained 54% of its original activity after 15 repeated catalytic reactions concerning its free form.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Modifying Thermostability and Reusability of Hyperthermophilic Mannanase by Immobilization on Glutaraldehyde Cross-Linked Chitosan Beads

et al. 2022

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“…The mixture was titrated to estimate the quantity of the produced EV (Equation ( 1)). The method by Dhiman et al [29] was adopted with minor modification to compare the thermal denaturation and thermodynamic parameters, such as activation and activation energy of free CRL and CRL/SiO 2 /Fe 3 O 4 ,/GO. The effect of temperature on both lipases was tested between 20 and 50 • C, where their lipase activities were monitored for 3 h. The first-order rate constants (k a ) were calculated using Equations ( 3) and (4):…”

Section: Regeneration Studymentioning

confidence: 99%

Operational Stability, Regenerability, and Thermodynamics Studies on Biogenic Silica/Magnetite/Graphene Oxide Nanocomposite-Activated Candida rugosa Lipase

2021

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Inorganic biopolymer-based nanocomposites are useful for stabilizing lipases for enhanced catalytic performance and easy separation. Herein, we report the operational stability, regenerability, and thermodynamics studies of the ternary biogenic silica/magnetite/graphene oxide nanocomposite (SiO2/Fe3O4/GO) as a support for Candida rugosa lipase (CRL). The X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), field-electron scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), and nitrogen adsorption/desorption data on the support and biocatalyst corroborated their successful fabrication. XPS revealed the Fe3O4 adopted Fe2+ and Fe3+ oxidation states, while XRD data of GO yielded a peak at 2θ = 11.67°, with the SiO2/Fe3O4/GO revealing a high surface area (≈261 m2/g). The fourier transform infrared (FTIR) spectra affirmed the successful fabricated supports and catalyst. The half-life and thermodynamic parameters of the superparamagnetic immobilized CRL (CRL/SiO2/Fe3O4/GO) improved over the free CRL. The microwave-regenerated CRL/SiO2/Fe3O4/GO (≈82%) exhibited higher catalytic activity than ultrasonic-regenerated (≈71%) ones. Lower activation (Ea) and higher deactivation energies (Ed) were also noted for the CRL/SiO2/Fe3O4/GO (13.87 kJ/mol, 32.32 kJ/mol) than free CRL (15.26 kJ/mol, 27.60 kJ/mol). A peak at 4.28 min in the gas chromatograph-flame ionization detection (GC-FID) chromatogram of the purified ethyl valerate supported the unique six types of 14 hydrogen atoms of the ester (CAS: 539-82-2) in the proton nuclear magnetic resonance (1H-NMR) data. The results collectively demonstrated the suitability of SiO2/Fe3O4/GO in stabilizing CRL for improved operational stability and thermodynamics and permitted biocatalyst regenerability.

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“…In terms of chemical modification, Dhiman et al (2019) modified sodium alginate with β-cyclodextrin by adding sodium alginate and β-cyclodextrin to sodium hydroxide solution. The mixture was further mixed under constant temperature in a water bath.…”

Section: Diversified Modification Capabilitiesmentioning

confidence: 99%

Application progress of microbial immobilization technology based on biomass materials

Zhang

Fan

Zhang

et al. 2021

BioRes

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In recent years, microbial degradation technology has shown broad potential in the fields of agriculture, industry, and environmental protection. However, in practical applications the technology still encounters many problems, such as low bacterial survivability during dynamic operations, the need to remove bacterial liquid, and low tolerance in high-toxic environments, among other issues. Immobilization technology has been developed to overcome such limitations. Microbial strains have been prepared for a specific range of activities utilizing self-fixation or exosome fixation. Immobilization can significantly improve strain density, toxicity tolerance, and bacterial liquid removal. This review first presents the advantages and disadvantages of the current microbial immobilization technologies and then summarizes the properties and characteristics of various carrier materials. The review focuses on how biomass-derived materials have been used as the carriers in new microbial immobilization technologies. The excellent biocompatibility, unique physical structure, and diversified modification methods of biomass-derived materials have shown excellent prospects in the field of microbial immobilization. Finally, microbial immobilization technologies’ potential applications in agriculture, industry, and environmental applications are considered.

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Immobilization of mannanase on sodium alginate-grafted-β-cyclodextrin: An easy and cost effective approach for the improvement of enzyme properties

Cited by 49 publications

References 31 publications

Modifying Thermostability and Reusability of Hyperthermophilic Mannanase by Immobilization on Glutaraldehyde Cross-Linked Chitosan Beads

Modifying Thermostability and Reusability of Hyperthermophilic Mannanase by Immobilization on Glutaraldehyde Cross-Linked Chitosan Beads

Operational Stability, Regenerability, and Thermodynamics Studies on Biogenic Silica/Magnetite/Graphene Oxide Nanocomposite-Activated Candida rugosa Lipase

Application progress of microbial immobilization technology based on biomass materials

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