“…The activity of immobilized lipase can be determined by titrating (with NaOH) the acetic acid produced in the hydrolysis of triacetin [23,24] or the fatty acids released from the hydrolysis of olive oil. Triacetin (34.15 g) and pH 7.7 phosphate buffer were gradually mixed under vigorous shaking to prepare an emulsion which was finally made up to a volume of 500 ml with pH 7.7 phosphate buffer.…”
“…The activity of immobilized lipase can be determined by titrating (with NaOH) the acetic acid produced in the hydrolysis of triacetin [23,24] or the fatty acids released from the hydrolysis of olive oil. Triacetin (34.15 g) and pH 7.7 phosphate buffer were gradually mixed under vigorous shaking to prepare an emulsion which was finally made up to a volume of 500 ml with pH 7.7 phosphate buffer.…”
“…This hydrolysis reaction (forward direction) needs fat and an aqueous environment, which can be favorably achieved when using meat processing or pet food wastewater as a substrate. There are several research studies available on the treatment of oily wastes with artificially added fats using free lipase (FL) [7][8][9][10][11][12]. However, most of these studies focused on the pretreatment of wastewater with artificially added fats at low concentrations (<1 g/L) and there is very little information available on oily wastewater treatment using immobilized lipase (IL).…”
“…Lipase activity was assayed by using "olive emulsification method" [12]. One activity unit (U) of lipase was defined as the amount of enzyme required to catalyze substrate olive oil to hydrolyze to produce 1 mol of free fatty acid per min at 40 • C and pH 8.0.…”
Section: Lipase Activity Assay and Protein Estimationmentioning
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