Effect of surface hydrophobicity/hydrophilicity of mesoporous supports on the activity of immobilized lipase

He, Jing; Xu, Yan; Ma, Hongan; Zhang, Qiong; Evans, David G.; Duan, Xue

doi:10.1016/j.jcis.2005.12.063

Cited by 56 publications

(25 citation statements)

References 33 publications

(37 reference statements)

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“…On the other hand, the co-condensation method between the same organic building block and the silica precursor leads in general to a more hydrophilic surface. [42,43] These ideas were confirmed because the hydrophilicity, measured by the nOH vibrations, and some associated framework modes were much larger in PMO 1 and PMO 2 than in MCM-41, PMS 1 , and PMS 2 . Therefore, one can control this property by choosing the method of synthesis.…”

Section: Resultsmentioning

confidence: 87%

Vibrational Study on the Local Structure of Post‐Synthesis and Hybrid Mesoporous Materials: Are There Fundamental Distinctions?

Vaz

Nunes

Vasconcellos-Dias

et al. 2007

Chemistry A European J

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Organic-inorganic mesoporous materials of the MCM-41 type are important materials that can be prepared by either post-synthesis or one-pot synthesis procedures. A complete control of the characteristics at a local level is of the utmost importance in view of the applications of such materials. However, there are not many studies relating such features with synthetic approaches. In this work, we prepared samples by post-synthesis derivatization of materials from Si-based MCM-41, with bidentate nitrogen ligands bearing one or two silylated arms, and by one-pot synthesis of organic-inorganic hybrid materials. The bulk properties of the two kinds of materials were comparable. Diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy and Raman spectroscopy were used to investigate the local environment, namely, the number of OH groups and distribution of SiO(4) units (large and small ring units). Hydrophilicity correlates with both the type of organic moiety used (mono- or disilylated), as well as with the synthetic procedure. The same vibrational studies showed how the structure in the channels changes as a function of pressure, reflecting the low mechanical stability of the mesoporous materials.

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Section: Resultsmentioning

confidence: 87%

Vibrational Study on the Local Structure of Post‐Synthesis and Hybrid Mesoporous Materials: Are There Fundamental Distinctions?

Vaz

Nunes

Vasconcellos-Dias

et al. 2007

Chemistry A European J

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“…[78][79][80] XRD analysis before and after adsorption of a protein can indicate retention of the mesoporous structure and also entry of the protein into the mesopores by a reduction in the intensity of the reflection peaks. [74,75,78,[81][82][83] …”

Section: Relative Size Of the Mesopores And The Proteinmentioning

confidence: 99%

“…Functionalization after immobilization of the protein can enhance the hydrophobicity and/or amphiphilicity of the support for effective substrate diffusion through the pores. [82,97] However, the solvent used for this reaction can reduce the activity of the immobilized protein. [41] Covalent coupling with surface amine functional groups on the support using glutaraldehyde, which can bind to enzyme amine groups, can increase the stability of the immobilized enzyme, significantly reduce the amount of leaching, and allow the immobilized enzyme to be reused.…”

Section: Surface Functional Groups Of Mps and Proteinmentioning

confidence: 99%

“…Recently this problem was overcome by polymerizing a methacrylate group onto the silicate after silanization to narrow the pore entrances even further. [61,82] Immobilization can also enhance storage stability, as mentioned with the cross-linked enzyme aggregates in Section 3.8. Trypsin immobilized on MCM-41 was still active after a week at 25 8C, whereas free trypsin had been completely inactivated; [27] trypsin immobilized on CNS also exhibited enhanced stability in aqueous and organic solvents.…”

Section: Reusability and Enhanced Stability Of The Immobilized Proteinmentioning

confidence: 99%

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Proteins in Mesoporous Silicates

2008

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Mesoporous silicates (MPS) have an ordered pore structure with dimensions comparable to many biological molecules. They have been extensively explored as supports for proteins and enzymes in biocatalytic applications. Since their initial discovery, novel syntheses methods have led to precise control over pore size and structure, particle size, chemical composition, and stability, thus allowing the adsorption of a wide variety of biological macromolecules, such as heme proteins, lipases, antibody fragments, and proteases, into their structures. This Review discusses the application of ordered, large-pore, functionalized mesoporous silicates to immobilize proteins for biocatalysis.

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“…It has also been used as a catalyst for the manufacture of anti-cancer drugs and even as an ingredient in cosmetics [57,83,99]. Yaropolov et al [156] proposed an amperometric electrode-based laccase for determining phenolics.…”

Section: Laccasementioning

confidence: 99%

Enzyme Immobilization by Amperometric Biosensors with TiO2 Nanoparticles Used to Detect Phenol Compounds

et al. 2015

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Nanoparticles of titanium dioxide (TiO 2 ) have unique properties in creating an appropriate microenvironment for immobilizing biomolecules without loss of biological activity, and facilitating electron transference between the enzyme and surface of the electrode. TiO 2 properties have led to its intensive use in building electrochemical biosensors. Another aspect is, the chemical process of sol-gel which offers new and interesting advantages in the encapsulation of biomolecules sensitive to heat and environmental conditions (enzymes, proteins, antibodies, and cells from plants, animals and micro-organisms), mainly due to a synthesized process at low temperatures. The nanomaterials produced by sol-gel have many advantages, including chemical inertia, physical rigidity, insignificant swelling in an aqueous medium, and porosity. For this reason, electrochemical biosensors consisting of nanomaterials have been extensively investigated and used in important industrial sectors, such as, those of pharmaceuticals, health, food, agriculture, and environment. They provide real-time data, which allows the control and traceability of each of the processes involved. Biosensors are devices that consist of one element of molecular recognition (biomolecules) and one transduction element. The objective of this work is to conduct a review of electrochemical biosensors using nanoparticles obtained from the sol-gel process and their potential application to measure phenol compounds.

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Effect of surface hydrophobicity/hydrophilicity of mesoporous supports on the activity of immobilized lipase

Cited by 56 publications

References 33 publications

Vibrational Study on the Local Structure of Post‐Synthesis and Hybrid Mesoporous Materials: Are There Fundamental Distinctions?

Vibrational Study on the Local Structure of Post‐Synthesis and Hybrid Mesoporous Materials: Are There Fundamental Distinctions?

Proteins in Mesoporous Silicates

Enzyme Immobilization by Amperometric Biosensors with TiO2 Nanoparticles Used to Detect Phenol Compounds

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