Immobilization of laccase on hairy polymer grafted zeolite particles: Degradation of a model dye and product analysis with MALDI–ToF-MS

Çelikbıçak, Ömür; Bayramoğlu, Gülay; Yılmaz, Meltem; Ersoy, Gozde; Bıçak, Niyazi; Salih, Bekir; Arıca, M. Yakup

doi:10.1016/j.micromeso.2014.08.003

Cited by 37 publications

(19 citation statements)

References 41 publications

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“…After 7 cycles, the immobilized laccase lost about 66.2% of initial activity. The loss of the activity was probably attributed to: (1) part of immobilized laccase with weak binding desorbed from the Mba support during the washing process for the instability of physical adsorption, (2) the enzyme activity may lost during storage 32 . In this study it took five days to finish the operational stability experiment since the reaction between immobilized laccase and ABTS was slow and continuously slowed down with prolonged the measurements.…”

Section: Resultsmentioning

confidence: 99%

Immobilizing Laccase on Different Species Wood Biochar to Remove the Chlorinated Biphenyl in Wastewater

Xia

Niu

et al. 2018

Sci Rep

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Biochars produced from two different wood species over a microwave assisted pyrolysis process were used as novel and green-based supports for immobilizing enzyme, laccase in particular. The results obtained from FT-IR, SEM and BET measurements indicated that Maple biochar with honeycomb structure has higher surface area and pore volume than Spruce biochar; and there exist O-H, C-H, C=O and C=C groups in biochars for potential chemical modification. The best laccase immobilization conditions identified from an orthogonal experiment were pH = 3, laccase concentration 16 g/L and contact time 8 h. Under such conditions, the high immobilization yield (64.2%) and amount (11.14 mg/g) of laccase on Maple biochar were achieved, leading to the significantly improved thermal stability of laccase. Moreover, the immobilized laccase is reusable and enhanced the enzymatic degradation of 4-hydroxy-3,5-dichlorobiphenyl (71.4% yield), thus creating a promising and novel type of adsorbent in the removal of polychlorinated biphenyls from wastewater.

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Section: Resultsmentioning

confidence: 99%

Immobilizing Laccase on Different Species Wood Biochar to Remove the Chlorinated Biphenyl in Wastewater

Xia

Niu

et al. 2018

Sci Rep

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“…This decline was mainly attributed to the accumulation of dye degradation products which depressed the activity of the enzyme and affected the reaction in the next cycle [ 13 ]. The other cause may be the loss of laccase and ABTS during the recycling and washing processes [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

“…In order to monitor the extent of MG degradation, changes in the absorbance spectra of the dye were recorded in the UV-visible range between 200 and 800 nm. After complete decolorization of MG, the supernatant was analyzed by MALDI-TOF-MS to identify the degradation products as previously described [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Immobilization of a Laccase/2,2’-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic Acid System to Layered Double Hydroxide/Alginate Biohybrid Beads for Biodegradation of Malachite Green Dye

Huang

Yang

Wang

et al. 2018

BioMed Research International

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The application of laccase-mediator-based catalysis is limited owing to the high cost of laccases and mediators and the potential toxicity of free mediators. Here, a novel biocatalyst (Im-LMS) was fabricated by immobilizing both laccase and a mediator (2,2'-azino-bis-[3-ethylbenzothiazoline]-6-sulfonic acid) on layered double hydroxide/alginate biohybrid beads. The catalytic activity of Im-LMS was evaluated for dye decolorization using malachite green. The decolorization yields of malachite green by Im-LMS and the free laccase-mediator system were 92% within 120 min and 90% within 90 min. Malachite green solution was detoxified completely after biodegradation by Im-LMS. Following eight reuse cycles of Im-LMS for dye treatment, a decolorization yield of 79% was obtained. The activity of Im-LMS was almost completely stable after being stored for 10 days. The recyclability and stability of Im-LMS will be helpful for reducing the running cost and potential toxicity associated with mediators to facilitate practical applications.

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“…Various studies on immobilized enzymes and their matrices (support phases) have been reported. Polymers, (1)(2)(3) zirconia, (4) zeolite, (5) chitosan, (6) and carbon nanoparticles (7) are some matrix materials that provide support to the binding of a particular enzyme. The immobilization principle is to remove the enzyme from the reaction mixture without any damage to its function and structure.…”

Section: Introductionmentioning

confidence: 99%

Development and Characterization of Superparamagnetic Nanomaterial to Determine Its Potential Application in Removing the Bitterness of Table Olives

Savaş¹,

Karaağaç²,

Köçkar³

et al. 2020

Sensors and Materials

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The aim of this study is to develop stable superparamagnetic iron oxide nanoparticles (SPIONs) for the immobilization of β-glucosidase. The goal is to figure out the possibility of applying immobilization techniques in the debittering of olives. In the present study, SPIONs with a size of ~9 nm were synthesized by co-precipitation, and immobilization was carried out by the formation of covalent bonds between β-glucosidase and SPIONs. The properties of the free enzymes immobilized with SPIONs were compared by using commercial β-glucosidase as a standard. The immobilized enzymes were characterized by using techniques including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The results showed that SPIONs are an effective and suitable material for the immobilization of β-glucosidase as well as a potential nanomaterial for the elimination of olive bitterness originating from oleuropein.

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Immobilization of laccase on hairy polymer grafted zeolite particles: Degradation of a model dye and product analysis with MALDI–ToF-MS

Cited by 37 publications

References 41 publications

Immobilizing Laccase on Different Species Wood Biochar to Remove the Chlorinated Biphenyl in Wastewater

Immobilizing Laccase on Different Species Wood Biochar to Remove the Chlorinated Biphenyl in Wastewater

Immobilization of a Laccase/2,2’-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic Acid System to Layered Double Hydroxide/Alginate Biohybrid Beads for Biodegradation of Malachite Green Dye

Development and Characterization of Superparamagnetic Nanomaterial to Determine Its Potential Application in Removing the Bitterness of Table Olives

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