Immobilization of a Laccase/2,2’-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic Acid System to Layered Double Hydroxide/Alginate Biohybrid Beads for Biodegradation of Malachite Green Dye

Abstract

The application of laccase-mediator-based catalysis is limited owing to the high cost of laccases and mediators and the potential toxicity of free mediators. Here, a novel biocatalyst (Im-LMS) was fabricated by immobilizing both laccase and a mediator (2,2'-azino-bis-[3-ethylbenzothiazoline]-6-sulfonic acid) on layered double hydroxide/alginate biohybrid beads. The catalytic activity of Im-LMS was evaluated for dye decolorization using malachite green. The decolorization yields of malachite green by Im-LMS and the free laccase-mediator system were 92% within 120 min and 90% within 90 min. Malachite green solution was detoxified completely after biodegradation by Im-LMS. Following eight reuse cycles of Im-LMS for dye treatment, a decolorization yield of 79% was obtained. The activity of Im-LMS was almost completely stable after being stored for 10 days. The recyclability and stability of Im-LMS will be helpful for reducing the running cost and potential toxicity associated with mediators to facilitate practical applications.

Environmentally Friendly and Recyclable Natural-Mediator-Modified Magnetic Nanoparticles for Laccase-Catalyzed Decolorization

“…Moreover, polyethylene glycol was modified with TEMPO, and the resulting immobilized redox mediator exhibited reusability for Lac-catalyzed dye decolorization [26]. Furthermore, efficient catalytic performance and easy recoverability were achieved by co-immobilization of Lac and a mediator in the same matrix [33,34]. In this work, the natural mediator SA was immobilized on magnetically separable MNPs to facilitate its recovery and reuse.…”

Medical Use of Polycatecholamines + Oxidoreductases-Modified Curdlan Hydrogels—Perspectives

“…For many reasons, related to bioremediation of various pollutants or medical applications, oxidoreductases have been immobilized on different hydrogels which provide appropriate and biocompatible environments for enzymatic reactions. For example, immobilized laccases from T. versicolor and C. unicolor were effectively applied for removal of persistent trace compounds in wastewaters (diclofenac, triclosan, paracetamol, bisphenol A, oils, dyes and other pollutants) [ 14 , 15 , 16 , 17 , 18 , 19 ]. Sampaio et al [ 20 ] proposed the bacterial nanocellulose-based hydrogel with immobilized commercial laccase.…”