Immobilization of laccase from Trametes versicolor on Lifetechtm supports for applications in degradation of industrial dyes

Abstract

In this study, immobilization of laccase from Trametes versicolor on eight Lifetech? supports, with different characteristics (pore size, length of the spacer arm and functional groups), was studied and optimized for intended use in bioremediation for decolorization of industrial wastewaters. Out of six tested amino-functionalized supports, the most promising carrier was proved to be porous Lifetech? ECR8309F with primary amino groups and a C2 spacer arm. Onto this support, laccase is attached by forming electrostatic interactions so that the most active preparation has shown the activity of 66876 U/g support. On the other hand, during immobilization of laccase on epoxy-functionalized Lifetech? ECR8285F, via hydrophobic interactions and covalent bonding confirmed by a desorption assay, immobilization yield of 60 % and the activity of 118929 U/g were accomplished. Furthermore, immobilized enzyme on this support showed high capacity for decolorization of dyes (Lanaset? Violet B, Lanaset? Blue 2R, bromothymol blue and bromocresol green), by combination of both adsorption and enzyme degradation. Decolorization was in the range of 88 to 96 % after 4 h, with more than 80 % achieved after only 45 min. Also, this preparation demonstrated high operational stability during seven consecutive reuses in all examined dye reaction systems.[Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. 451-03-68/2020-14/200135 and Grant no. 451-03-68/2020-14/ 200287]

Effects of Ionic Liquids on Metalloproteins

“…In addition to plant sources, fungi are a common source of laccase, and most fungi produce different laccase isoforms and isoenzymes. One of the most commonly studied forms of laccase is derived from the Trametes versicolor fungus [ 136 , 137 , 138 , 139 ]. The T. versicolor laccase contains two copper sites, a mono-copper and a tri-copper site ( Figure 1 ).…”

“…Trametes versicolor fungus [136][137][138][139]. The T. versicolor laccase contains two copper sites, a mono-copper and a tri-copper site (Figure 1).…”