Effects of Ionic Liquids on Metalloproteins

Abstract

In the past decade, innovative protein therapies and bio-similar industries have grown rapidly. Additionally, ionic liquids (ILs) have been an area of great interest and rapid development in industrial processes over a similar timeline. Therefore, there is a pressing need to understand the structure and function of proteins in novel environments with ILs. Understanding the short-term and long-term stability of protein molecules in IL formulations will be key to using ILs for protein technologies. Similarly, ILs have been investigated as part of therapeutic delivery systems and implicated in numerous studies in which ILs impact the activity and/or stability of protein molecules. Notably, many of the proteins used in industrial applications are involved in redox chemistry, and thus often contain metal ions or metal-associated cofactors. In this review article, we focus on the current understanding of protein structure-function relationship in the presence of ILs, specifically focusing on the effect of ILs on metal containing proteins.

Application progress of magnetic molecularly imprinted polymers chemical sensors in the detection of biomarkers

“…Ionic liquids (IL S ) are environmentally friendly functional monomers, which have attracted extensive attention due to their excellent stability and hydrolysis resistance. 112 Fig. 3B shows a novel Fe 3 O 4 @dopamine/graphene oxide modified with ionic liquids/β-cyclodextrin (ILs-Fe 3 O 4 @DA/GO/β-CD) SMIPs, which combined with a CL sensor, realized the detection of lysozyme in urine samples.…”

Effect of Hydrated Ionic Liquid on Photocycle and Dynamics of Photoactive Yellow Protein

“…The interaction between ionic liquids (ILs) and proteins has attracted significant interest. Numerous researchers have studied how proteins are dissolved in ILs and how cations or anions modify the secondary- or higher-order protein structures [ 1 , 2 , 3 , 4 , 5 , 6 ]. In particular, the role of the alkyl chain length of the IL cations and their relationship with the Hofmeister series have been discussed using various spectroscopic techniques.…”

Effects of Ionic Liquids on Laccase from Trametes versicolor

“…The protein folding process normally occurs in cells under native cytoplasmic conditions; however, extensive investigation on protein denaturation or unfolding has been carried out in vitro and has yielded significant insights into the thermodynamics of protein structural stability. In the context of ionic liquids, more and more proteins are under investigation for interactions with ionic liquids [19]. This is a challenging problem, as there are seemingly infinite combinations of ionic liquids and proteins to investigate.…”