2003
DOI: 10.1002/jsfa.1390
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Immobilization of invertase on celite and on polyacrylamide by an absorption procedure

Abstract: Invertase from Saccharomyces cerevisiae was immobilized on celite and on polyacrylamide by an absorption procedure. The properties of the immobilized invertase were characterized and compared with those of soluble invertase. The activity yield for immobilized invertase on celite and on polyacrylamide was 92% and 81% respectively. The optimum pH and temperature for both soluble and immobilized invertase activity were 4.6 and 60 • C respectively. The activity of immobilized invertase is stable in the range pH 4.… Show more

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Cited by 30 publications
(16 citation statements)
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References 13 publications
(6 reference statements)
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“…The highest activity for invertase and glucose oxidase was observed at pH 4.5 (0.041 mg TRS/min mL) and pH 5.5 (11.5×10 −4 mg H 2 O 2 /min mL), respectively. Both pH values are in agreement with those found in the literature [17][18][19][20].…”
Section: Resultssupporting
confidence: 92%
“…The highest activity for invertase and glucose oxidase was observed at pH 4.5 (0.041 mg TRS/min mL) and pH 5.5 (11.5×10 −4 mg H 2 O 2 /min mL), respectively. Both pH values are in agreement with those found in the literature [17][18][19][20].…”
Section: Resultssupporting
confidence: 92%
“…Since it is well known that all the protein that added to the matrix is not bound. Mansour and Dawoud (2003) suggested that increasing the enzyme loading above the saturation level resulted in the gradual decrease in the enzyme activity. This is because a high concentration of enzyme on the external surface can contribute to internal diffusional restrictions, thus leading to the underestimation of the initial activity of the immobilized enzyme (Wilson et al .…”
Section: Resultsmentioning
confidence: 99%
“…This is probably due to optimal adsorption of the enzyme on the matrix surface at 250 μg/5 ml for MCM-41. Mansour and Dawoud (2003) suggested that the gradual decrease in the enzyme activity is probably due to hindrance between the adsorbed enzyme molecules on the matrix, at higher concentrations of enzyme.…”
Section: Effect Of the Variation Of The Tyrosinase Concentration On Imentioning
confidence: 98%