Immobilization of <i>Candida antarctica</i> lipase B (CALB) on surface-modified rice husk ashes (RHA) via physical adsorption and cross-linking methods

Ulker, Cansu; Gokalp, Nurefsan; Güvenilir, Yüksel

doi:10.1080/10242422.2016.1247818

Cited by 22 publications

(8 citation statements)

References 20 publications

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“…Previously, successful performance of Im-CALB for PCL synthesis have been shown and suggested as an alternative for Nov-435 [14]. In the current work, ROP of δ-VL was performed at varied reaction temperatures and periods via Nov-435 and Im-CALB.…”

Section: Synthesis Of Poly(δ-valerolactone) Homopolymers and Their Spmentioning

confidence: 95%

“…In the present work, Nov-435, and CALB immobilized onto rice husk ashes (RHA) via physical adsorption (obtained in our previous work) were used as biocatalysts for ROP of δ-VL [13]. It was aimed to introduce the new home-made enzyme, that was shown to be successful for PCL (Mn = 14000 g/mol) and poly(ε-caprolactoneco-ω-pentadecalactone) (Mn = 20960 g/mol) syntheses previously, as an alternative for the commercial one [14,15]. In previous studies, performances of Im-CALB and Nov-435 had been compared for PCL and poly(ε-caprolactone-co-ω-pentadecalactone) syntheses.…”

Section: Introductionmentioning

confidence: 99%

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Performance Comparison of Commercial and Home-Made Lipases for Synthesis of Poly(δ-valerolactone) Homopolymers

Ulker¹,

Gok²,

Güvenilir³

2019

Journal of Renewable Materials

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Novozyme 435, which is the commercially available immobilized form of Candida antarctica lipase B, has been successfully conducted ring opening polymerization of lactones in organic solvents. In this paper, it was aimed to introduce an alternative biocatalyst for Novozyme 435. Candida antarctica lipase B immobilized onto rice husk ashes via physical adsorption (with a specific activity of 4.4 U/mg) was prepared in previous studies and used as a biocatalyst for poly(δ-valerolactone) synthesis in the present work. Polymerization reactions were proceeded at various reaction temperatures and periods via both two immobilized enzyme preparations. The resulting products were characterized spectroscopically and thermally. The highest molecular weight (Mn = 9010 g/mol) was obtained via Novozyme 435 catalysis at 40℃ and 24 hours. The performance of home-made lipase, which resulted in a molecular weight of 8040 g/mol, was close to commercial one.

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Section: Synthesis Of Poly(δ-valerolactone) Homopolymers and Their Spmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Performance Comparison of Commercial and Home-Made Lipases for Synthesis of Poly(δ-valerolactone) Homopolymers

Ulker¹,

Gok²,

Güvenilir³

2019

Journal of Renewable Materials

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“…Recently, RHA has been used as a carrier, using cross-linking and adsorption methods, for the recovery of activity of Candida antarctica lipase B (CALB) with good results, also increasing stability, reusability and application adaptability of the biocatalyst (Ulker et al 2016a) and applied in the biosynthesis of poly(e-caprolactone) (Ulker et al 2016b).…”

Section: Introductionmentioning

confidence: 99%

Rice husk ash as a potential carrier for the immobilization of lipases applied in the enzymatic production of biodiesel

Bonet-Ragel

López-Pou

Tutusaus

et al. 2017

Biocatalysis and Biotransformation

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The potential application of rice husk ash (RH26) as support for the immobilization of a recombinant Rhizopus oryzae (rROL) lipase as biocatalyst in the enzymatic biodiesel production using alperujo oil and the comparison with commercial hydrophobic support OD403 (RelOD) has been made. Although the specific activity (UA mg support À1 ) was around one-half lower in RH26 than in RelOD when they were used as biocatalyst in biodiesel reaction, the normalized initial rate was similar, between 1.6 and 2.4 lmol FAME mL À1 mL À1 UA À1 . Thus in terms of biocatalysis performance, rice husk as is an alternative to commercial supports. However, the main problem is the more complex recovery of RH26 for the reutilization compared with commercial ones. ARTICLE HISTORY

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“…Surfaced-modified RHA samples were thermally characterized by TGA in order to observe the presence of silane coupling agents. The cumulative weight loss of pure RHA up to this temperature was given as 1.8% in a previous study [33]. The thermogram given in Figure 4 showed a cumulative weight loss of 2.5% for 3-APTMS modified RHA as the temperature reaches 650°C .…”

Section: Characterization Of Rha and Activated Rhamentioning

confidence: 60%

Enzymatic Synthesis of Polycaprolactone: Effect of Immobilization Mechanism of CALB on Polycaprolactone Synthesis

Kaptan¹,

Sc²,

Avcıbaşı-Güvenilir³

2018

Journal of Renewable Materials

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Surface-modified rice husk ash was used as an inorganic support material for immobilization of Candida antarctica lipase B. (3-aminopropyl) trimethoxysilane was used for surface modification. Immobilization of CALB was performed via both physical adsorption and cross-linking. PCL synthesis was carried out by using these immobilized enzymes, free enzyme and Novozyme 435 ®. Molecular weight distribution of polymer samples was obtained by gel permeation chromatography (GPC) and chain structures of the polymer samples were observed by hydrogen nuclear magnetic resonance (1 H-NMR). The highest monomer conversion is generally obtained by using cross-linked enzyme, around 90%. PDI values for all polymer samples were approximately 1.5 which can be considered as acceptable. In general cross-linked enzymes were better than physically adsorbed enzymes in terms of average molecular weights. It can be concluded that PCL can be synthesized with these immobilized enzymes with high molecular weight and low PDI values.

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Immobilization of Candida antarctica lipase B (CALB) on surface-modified rice husk ashes (RHA) via physical adsorption and cross-linking methods

Cited by 22 publications

References 20 publications

Performance Comparison of Commercial and Home-Made Lipases for Synthesis of Poly(δ-valerolactone) Homopolymers

Performance Comparison of Commercial and Home-Made Lipases for Synthesis of Poly(δ-valerolactone) Homopolymers

Rice husk ash as a potential carrier for the immobilization of lipases applied in the enzymatic production of biodiesel

Enzymatic Synthesis of Polycaprolactone: Effect of Immobilization Mechanism of CALB on Polycaprolactone Synthesis

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