Immobilization of Bacillus amyloliquefaciens MBL27 cells for enhanced antimicrobial protein production using calcium alginate beads

Abstract

Cell immobilization is one of the common techniques for increasing the overall cell concentration and productivity. Bacillus amyloliquefaciens MBL27 cells were immobilized in calcium alginate beads and it is a promising method for repeated AMP (antimicrobial protein) production. The present study aimed at determining the optimal conditions for immobilization of B. amyloliquefaciens MBL27 cells in calcium alginate beads and the operational stability for enhanced production of the AMP. AMP production with free and immobilized cells was also done. In batch fermentation, maximum AMP production (7300 AU (arbitrary units)/ml against Staphylococcus aureus) was obtained with immobilized cells in shake flasks under optimized parameters such as 3% (w/v) sodium alginate, 136 mM CaCl2 with 350 alginate beads/flask of 2.7-3.0 mm diameter. In repeated cultivation, the highest activity was obtained after the second cycle of use and approx. 94% production was noted up to the fifth cycle. The immobilized cells of B. amyloliquefaciens MBL27 in alginate beads are more efficient for the production of AMP and had good stability. The potential application of AMP as a wound healant and the need for development of economical methods for improved production make whole cell immobilization an excellent alternative method for enhanced AMP production.

Cell‐Laden Hydrogels for Multikingdom 3D Printing

“…Living materials are a class of materials that feature an interface of active, living cells within a polymeric network serving as an enclosure or matrix in which the cells reside. [1][2][3][4][5][6][7] Such materials offer a diverse series of possible applications, ranging from sensors and wearable devices to fermentation bioreactors. [1,2,6,7] Ideally, these materials should be easy to handle and process, promote viability and longevity of the enclosed cells, and produce physically robust structures for extended use.…”

Swelling and drug release behavior of calcium alginate/poly (sodium acrylate) hydrogel beads

“…[7][8][9][10] However calcium alginate beads do not exhibit fair stability in the physiological fluid or phosphate buffer of pH 7.4. [11,12] The presence of counter ions in the swelling medium induces ion exchange between cross-linking Ca ++ ions that are present within the so called 'egg-box' cavity, which finally results in the degradation of alginate beads within a course of few hours. In addition, the transition of drug-loaded calcium alginate beads from stomach to large intestine consists of passage of drugloaded oral formulations via the simulating gastric fluid (SGF) of pH 1.0 for 2-3 h, followed by their transfer in simulating intestinal fluid (SIF) of pH 7.4.…”

Compartmentalized microbes and co-cultures in hydrogels for on-demand bioproduction and preservation

“…Immobilized cell technologies, wherein microbes are encapsulated within a polymeric matrix, have been developed as an alternative to suspension cell culture [12][13][14][15][16] . These microbe-laden matrices have been used to investigate quorum sensing between microbial species 14 , and as 3D architected 'living materials' [15][16][17] .…”