Immobilization of gold nanoparticles on cell culture surfaces for safe and enhanced gold nanoparticle-mediated laser transfection

Kalies, Stefan; Heinemann, Dag; Schomaker, Markus; Gentemann, Lara; Meyer, Heiko; Ripken, Tammo

doi:10.1117/1.jbo.19.7.070505

Cited by 15 publications

(17 citation statements)

References 11 publications

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“…The GNOME-LP technology was previously found as a high-throughput, cell friendly and rapid method for a transient cell permeabilization allowing an uptake of molecules, like dyes or siRNA into cells 32 , 35 , 36 , 56 . The gentle cell permeabilization was achieved by heat generated through the interaction of laser pulses and gold nanoparticles randomly adherent on the cells.…”

Section: Discussionmentioning

confidence: 99%

“…The gentle cell permeabilization was achieved by heat generated through the interaction of laser pulses and gold nanoparticles randomly adherent on the cells. In these experiments, gold nanoparticles and energy density of the applied laser beam were adjusted to achieve a transient permeabilization 32 , 42 , 56 . In consideration of the survival, patch-clamp experiments suggested that the repair of the cells after GNOME-LP treatment had two components.…”

Section: Discussionmentioning

confidence: 99%

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Functionalization of gold-nanoparticles by the Clostridium perfringens enterotoxin C-terminus for tumor cell ablation using the gold nanoparticle-mediated laser perforation technique

Becker

Leskau

Schlingmann-Molina

et al. 2018

Sci Rep

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A recombinant produced C-terminus of the C. perfringens enterotoxin (C-CPE) was conjugated to gold nanoparticles (AuNPs) to produce a C-CPE-AuNP complex (C-CPE-AuNP). By binding to claudins, the C- CPE should allow to target the AuNPs onto the claudin expressing tumor cells for a subsequent cell killing by application of the gold nanoparticle-mediated laser perforation (GNOME-LP) technique. Using qPCR and immunocytochemistry, we identified the human Caco-2, MCF-7 and OE-33 as well as the canine TiHoDMglCarc1305 as tumor cells expressing claudin-3, -4 and -7. Transepithelial electrical resistance (TEER) measurements of Caco-2 cell monolayer showed that the recombinant C-CPE bound to the claudins. GNOME-LP at a laser fluence of 60 mJ/cm2 and a scanning speed of 0.5 cm/s specifically eliminated more than 75% of claudin expressing human and canine cells treated with C-CPE-AuNP. The same laser fluence did not affect the cells when non-functionalized AuNPs were used. Furthermore, most of the claudin non-expressing cells treated with C-CPE-AuNP were not killed by GNOME-LP. Additionally, application of C-CPE-AuNP to spheroids formed by MCF-7 and OE-33 cells grown in Matrigel reduced spheroid area. The results demonstrate that specific ablation of claudin expressing tumor cells is efficiently increased by activated C-CPE functionalized AuNPs using optical methods.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Functionalization of gold-nanoparticles by the Clostridium perfringens enterotoxin C-terminus for tumor cell ablation using the gold nanoparticle-mediated laser perforation technique

Becker

Leskau

Schlingmann-Molina

et al. 2018

Sci Rep

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“…Thereby, various laser parameters, including resonant and off-resonant irradiation of the gold nanoparticles, were taken into account. [20][21][22][23][24] However, the biological consequences of this approach and other laser transfection procedures are still not well understood. Addressing this, we previously analyzed cell membrane potential changes by patch clamp analysis in off-resonant gold nanoparticle-mediated laser transfection.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the cellular response triggered by gold nanoparticle–mediated laser manipulation

et al. 2015

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Laser-based transfection techniques have proven high applicability in several cell biologic applications. The delivery of different molecules using these techniques has been extensively investigated. In particular, new high-throughput approaches such as gold nanoparticle–mediated laser transfection allow efficient delivery of antisense molecules or proteins into cells preserving high cell viabilities. However, the cellular response to the perforation procedure is not well understood. We herein analyzed the perforation kinetics of single cells during resonant gold nanoparticle–mediated laser manipulation with an 850-ps laser system at a wavelength of 532 nm. Inflow velocity of propidium iodide into manipulated cells reached a maximum within a few seconds. Experiments based on the inflow of FM4-64 indicated that the membrane remains permeable for a few minutes for small molecules. To further characterize the cellular response postmanipulation, we analyzed levels of oxidative heat or general stress. Although we observed an increased formation of reactive oxygen species by an increase of dichlorofluorescein fluorescence, heat shock protein 70 was not upregulated in laser-treated cells. Additionally, no evidence of stress granule formation was visible by immunofluorescence staining. The data provided in this study help to identify the cellular reactions to gold nanoparticle–mediated laser manipulation.

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“…Effective cell permeabilization with a precise control of cell treatment allows applying AuNPs’ optical transfection: an effective and accurate method with many potential applications both in vivo and in the clinic. The ability to deliver DNA/RNA/siRNA into mammalian cells using AuNSps and AuNRs was demonstrated by Ripken’s [ 121 , 122 ], Chia-Chun Chen et al’s [ 123 ] and Dholakia’s research groups [ 124 ]. Even more precise treatment can be achieved by single-cell transfection techniques [ 125 , 126 ], which enable the individual monitoring and performing of genetic changes in a specific cell, without treatment of other cells.…”

Section: Aunp Mediated Optoporationmentioning

confidence: 99%

Towards Effective Photothermal/Photodynamic Treatment Using Plasmonic Gold Nanoparticles

Bucharskaya

Maslyakova

Terentyuk

et al. 2016

IJMS

119

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Gold nanoparticles (AuNPs) of different size and shape are widely used as photosensitizers for cancer diagnostics and plasmonic photothermal (PPT)/photodynamic (PDT) therapy, as nanocarriers for drug delivery and laser-mediated pathogen killing, even the underlying mechanisms of treatment effects remain poorly understood. There is a need in analyzing and improving the ways to increase accumulation of AuNP in tumors and other crucial steps in interaction of AuNPs with laser light and tissues. In this review, we summarize our recent theoretical, experimental, and pre-clinical results on light activated interaction of AuNPs with tissues and cells. Specifically, we discuss a combined PPT/PDT treatment of tumors and killing of pathogen bacteria with gold-based nanocomposites and atomic clusters, cell optoporation, and theoretical simulations of nanoparticle-mediated laser heating of tissues and cells.

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Immobilization of gold nanoparticles on cell culture surfaces for safe and enhanced gold nanoparticle-mediated laser transfection

Cited by 15 publications

References 11 publications

Functionalization of gold-nanoparticles by the Clostridium perfringens enterotoxin C-terminus for tumor cell ablation using the gold nanoparticle-mediated laser perforation technique

Functionalization of gold-nanoparticles by the Clostridium perfringens enterotoxin C-terminus for tumor cell ablation using the gold nanoparticle-mediated laser perforation technique

Characterization of the cellular response triggered by gold nanoparticle–mediated laser manipulation

Towards Effective Photothermal/Photodynamic Treatment Using Plasmonic Gold Nanoparticles

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