Immobilization of glucose oxidase in cross-linked poly(acrylamide/acrylic acid) microgels

Cabarcos, E. López; Rubio-Retama, Jorge; López-Ruíz, Beatríz

doi:10.1007/b94016

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…The biosensor response was also affected by the quantity of microparticles immobilized at the surface of the electrode that acts as transducer of the biosensor. The same group reported the possibility of GOx immobilization in similar microgel systems 138–140. The immobilization of the enzyme was effective when the number of crosslinks was equal or greater than 0.021 mol g −1 .…”

Section: Synthesis Characterization and Applications Of Composite MImentioning

confidence: 97%

Composite aqueous microgels: an overview of recent advances in synthesis, characterization and application

Pich

Adler

2006

Polymer International

104

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In the last decade there has been a steady increase in the number of publications describing the synthesis, characterization and applications of hybrid particles. Colloidal aqueous microgels form an important subdivision of polymer colloids used for the deposition of different functional materials. Due to their numerous attractive properties, microgel particles have been used as templates for the synthesis, storage and transportation of nanostructured materials. This paper reviews the important developments in the area of composite microgels over the last decade, and is limited to microgel particles operating in aqueous media. The patent literature is not included in the review. Different synthetic approaches as well as some properties and applications of composite microgels are discussed.

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Section: Synthesis Characterization and Applications Of Composite MImentioning

confidence: 97%

Composite aqueous microgels: an overview of recent advances in synthesis, characterization and application

Pich

Adler

2006

Polymer International

104

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“…A unique feature of the proposed methodology was spatial focusing of PAA/enzyme co-electrodeposition on the microscopic structures, which is not possible in manual procedures. Further tests with a library of PAA/EDP variants from combinatorial synthesis proved that the substrate response characteristics of resulting PAA/EDP-based glucose biosensors were related to the degree of hydrophobicity and the swelling properties of the PAA polymer matrix. , Other examples of successful PAA-based enzyme biosensors, mostly using glucose oxidase (GO x ) as enzyme model, involved polyacrylamide/PAA, polyaniline/polyacrylonitrile/PAA, polyaniline/PAA, polytetrafluoroethylene/PAA, silica/ferrocene-tagged PAA, graphite oxide-polyethyleneimine/PAA, and composites or thin films of PAA hydrogels as electrode coatings for biocatalyst immobilization. Existing biosensor design involving PAA involves chemical synthesis steps to make the polymer and functional additives and thus requires appropriate skills and facilities.…”

Section: Introductionmentioning

confidence: 99%

Cheap and Sustainable Biosensor Fabrication by Enzyme Immobilization in Commercial Polyacrylic Acid/Carbon Nanotube Films

2022

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Novel glucose biosensors were constructed by loading glucose oxidase (GO x ) into the nanopores of homogenous carbon nanotube (CNT) films on the surface of Pt disk electrodes and trapping the enzyme by subsequent deposition of polyacrylic acid (PAA), forming PAA/GO x -CNT-modified Pt disks. In amperometric biosensing with anodic hydrogen peroxide (H 2 O 2 ) detection at a potential of +600 mV, increasing electrolyte glucose concentrations produced instantaneous steps in the H 2 O 2 oxidation current. Glucose biosensor amperometry was feasible down to 10 μM, with a sensitivity of about 34 μA mM –1 cm –2 and linear current response up to 5 mM. The biosensors reliably determined glucose concentrations in human serum and a beverage. Successful trials with PAA/GO x -CNT-modified screen-printed Pt electrode disks demonstrated the potential of this means of enzyme fixation in biosensor mass fabrication, which offers a unique combination of cheap availability of the two matrix constituents and sensor layer formation through simple drop-and-dry steps. PAA/GO x -CNT/Pt biosensors are green and user-friendly bioanalytical tools that do not need large budgets, special skills, or laboratory amenities for their production. Any user, from industrial, university, or school laboratories, even if inexperienced in biosensor construction, can prepare functional biosensors with GO x , as in these proof-of-principle studies, or with other redox enzymes, for clinical, environmental, pharmaceutical, or food sample analysis.

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Enzymes encapsulated smart polymer micro assemblies and their tuned multi-functionalities: a critical review

Naseem,

Arif,

Ahmad Haral

et al. 2023

International Journal of Polymeric Materials and Polymeric Biom

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Immobilization of glucose oxidase in cross-linked poly(acrylamide/acrylic acid) microgels

Cited by 3 publications

References 12 publications

Composite aqueous microgels: an overview of recent advances in synthesis, characterization and application

Composite aqueous microgels: an overview of recent advances in synthesis, characterization and application

Cheap and Sustainable Biosensor Fabrication by Enzyme Immobilization in Commercial Polyacrylic Acid/Carbon Nanotube Films

Enzymes encapsulated smart polymer micro assemblies and their tuned multi-functionalities: a critical review

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