Immobilization of erythropoietin to culture erythropoietin-dependent human leukemia cell line

Ito, Yoshihiro; Hasuda, Hirokazu; Yamauchi, Tetsuya; Komatsu, Norio; Ikebuchi, Kenji

doi:10.1016/j.biomaterials.2003.09.002

Cited by 50 publications

(32 citation statements)

References 39 publications

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“…We previously prepared ultraviolet-curable gelatin, which was derivatized with phenylazido groups, and micropatterned the surface by lithography [27,28]. This study reveals that the micropatterning can be produced by visible light irradiation.…”

Section: Cell Adhesionmentioning

confidence: 96%

Visible light-induced crosslinkable gelatin

et al. 2010

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Section: Cell Adhesionmentioning

confidence: 96%

Visible light-induced crosslinkable gelatin

et al. 2010

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“…2.1 Photoreactive preparation and the detection of AzPhIFN-α [8][9][10][11][12][13][14][15][16][17][18][19] All treatments were carried out in the dark. In a series of experiments, reagents were dissolved in deionized water in ice bath.…”

Section: Methodsmentioning

confidence: 99%

Preparation and activity of a nanometer anti-microbial polyurethane

Guan

Tao

et al. 2009

J. Wuhan Univ. Technol.-Mat. Sci. Edit.

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A novel way for photo-immobilization with interferon-α (IFN-α) on the surface of polyurethane (PU) material, which makes PU condom more effective function in the future, was investigated. A kind of photoactive arylazide-4-azidobenzoic acid was modified to IFN-α with the confirmation of IR and Raman spectrum. Micro morphology of the photo-immobilized cell factors was observed by FESEM as well as CSLM at the level of nanometer. Finally, the product with elementary test of anti-microbial was also evaluated .

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“…Considering that porcine gelatin has 11.4 wt% of carboxyl groups of glutamic acid (4.2%) and aspartic acid (7.2%), we concluded that about 14% of these groups were coupled with 3-aminopropylphosphonic acid. We had previously incorporated azidophenyl or furan groups into gelatin in a similar manner, and found that the contents were 84% 44 and near 100%, 45 respectively. The low reactivity with phosphate was considered to be caused by ionic repulsion of the phosphate group.…”

Section: Preparation Of Phosphonated Gelatinmentioning

confidence: 99%

Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement

Zhou¹,

Park²,

Mao³

et al. 2015

IJN

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Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by 31 P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

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Immobilization of erythropoietin to culture erythropoietin-dependent human leukemia cell line

Cited by 50 publications

References 39 publications

Visible light-induced crosslinkable gelatin

Visible light-induced crosslinkable gelatin

Preparation and activity of a nanometer anti-microbial polyurethane

Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement

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