Immobilization of DNA Hydrogel Plugs in Microfluidic Channels

Olsen, Kimberly G.; Ross, David; Tarlov, Michael J.

doi:10.1021/ac0156969

Cited by 102 publications

(135 citation statements)

References 30 publications

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“…The advent of soft lithography using materials such as poly(dimethylsiloxane) (PDMS) opened new avenues for chip construction by using simple molding techniques for microstructuring and not requiring high-temperature bonding [14][15][16]. Occasionally, other materials such as synthetic polymers [14] including polyacrylic, polycarbonate, polystyrene, cellulose acetate, and poly(ethylene terephthalate), as well as ceramics substrates, were proposed for fluidic channel construction [14,17,18]. In the case of synthetic polymers, injection molding is a preferred method for putative mass-production, while channel sealing can be accomplished by means of a plastic foil [14].…”

Section: Introductionmentioning

confidence: 99%

New advances in microchip fabrication for electrochromatography

Szekeres

Guttman

2005

Electrophoresis

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There is a great demand in separation technologies for faster and more effective analysis processes. Miniaturization is a suitable technique for satisfying this demand as reduction in size gives increased separation speed with higher efficiency. CEC is an electric-field-mediated separation technique where the liquid flow is generated by the electric field itself. The main advantage of using electric field over pressure for flow generation is the flat flow profile of the EOF; thus, CEC is one of the best candidates to construct a novel and high-efficiency microanalytical device. The aim of the present paper is to review the basic fabrication and bonding principles, as well as connection and system integration options for microfluidics-based electrochromatography. The physical structure and fluidic channel formation are critically evaluated, including glass microstructuring and fusion bonding. Recent developments in nanoflow measurements and the application of various flow control units are also extensively discussed.

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Section: Introductionmentioning

confidence: 99%

New advances in microchip fabrication for electrochromatography

Szekeres

Guttman

2005

Electrophoresis

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“…͑corresponding to C-erbB-2͒ at 10 M was covalently immobilized in the polyacrylamide hydrogel plugs within chambers 1a and 1b. [26][27][28][29] Similarly, capture molecule 2 ͑corresponding to nm23͒ was immobilized in chamber 2. Saturated drug segments A and B were hybridized with capture molecule 1 in chamber 1b and capture molecule 2 in chamber 2, respectively.…”

Section: Experiments and Resultsmentioning

confidence: 99%

“…1ϫ TE and 0.5M NaCl was chosen as the buffer because it is a kind of high-salt buffer that can assure the high hybridization rate. [26][27][28][29] Here, 2 M was set as the normal concentration of C-erbB-2 and nm23. When 5 l of FIG.…”

Section: Experiments and Resultsmentioning

confidence: 99%

“…When the concentration of C-erbB-2 was above 2 M, the velocity of capture would be raised. 26 After electrophoresed 3 min, the over-expressed C-erbB-2 ͑10 M͒ was moved out of the hydrogel microplug in chamber 1a and then electrophoresed into chamber 1b and displaced some drug segment A ͑8 min experientially͒. This part of drug segment A was then moved into reservoir 5 and finally transferred through chamber 4 to reservoir 13 ͑10 min experientially͒.…”

Section: -4 Zhang Et Al Biomicrofluidics 3 044105 ͑2009͒mentioning

confidence: 99%

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A microfluidic DNA computing processor for gene expression analysis and gene drug synthesis

Qin

Lin

2009

Biomicrofluidics

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Boolean logic performs a logical operation on one or more logic input and produces a single logic output. Here, we describe a microfluidic DNA computing processor performing Boolean logic operations for gene expression analysis and gene drug synthesis. Multiple cancer-related genes were used as input molecules. Their expression levels were identified by interacting with the computing related DNA strands, which were designed according to the sequences of cancer-related genes and the suicide gene. When all the expressions of the cancer-related genes fit in with the diagnostic criteria, positive diagnosis would be confirmed and then a complete suicide gene ͑gene drug͒ could be synthesized as an output molecule. Microfluidic chip was employed as an effective platform to realize the computing process by integrating multistep biochemical reactions involving hybridization, displacement, denaturalization, and ligation. By combining the specific design of the computing related molecules and the integrated functions of the microfluidics, the microfluidic DNA computing processor is able to analyze the multiple gene expressions simultaneously and realize the corresponding gene drug synthesis with simplicity and fast speed, which demonstrates the potential of this platform for DNA computing in biomedical applications.

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“…The primary advantage of polymer substrates lies in the ability for mass production at low costs based on replication (casting, embossing, imprinting, or injection molding) techniques for the possible disposable use [7]. Several important polymers including poly(dimethylsiloxane) (PDMS) [8][9][10][11], polymethylmethacrylate (PMMA) [12][13][14][15], polycarbonate [16,17], and polystyrene [18,19] are now increasingly used to fabricate microfluidic systems. In particular, PDMS is a soft polymer that is being actively developed for miniaturized bioassays due to its desirable features including easy replica molding, good optical transparency (down to 230 nm), no toxicity to proteins and cells, as well as easy irreversibly or reversibly sealing to itself and other materials [9,[20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

A polymeric master replication technology for mass fabrication of poly(dimethylsiloxane) microfluidic devices

Lin

et al. 2005

Electrophoresis

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A polymeric master replication technology for mass fabrication of poly(dimethylsiloxane) microfluidic devicesA protocol of producing multiple polymeric masters from an original glass master mold has been developed, which enables the production of multiple poly(dimethylsiloxane) (PDMS)-based microfluidic devices in a low-cost and efficient manner. Standard wetetching techniques were used to fabricate an original glass master with negative features, from which more than 50 polymethylmethacrylate (PMMA) positive replica masters were rapidly created using the thermal printing technique. The time to replicate each PMMA master was as short as 20 min. The PMMA replica masters have excellent structural features and could be used to cast PDMS devices for many times. An integration geometry designed for laser-induced fluorescence (LIF) detection, which contains normal deep microfluidic channels and a much deeper optical fiber channel, was successfully transferred into PDMS devices. The positive relief on seven PMMA replica masters is replicated with regard to the negative original glass master, with a depth average variation of 0.89% for 26 mm deep microfluidic channels and 1.16% for the 90 mm deep fiber channel. The imprinted positive relief in PMMA from master-to-master is reproducible with relative standard deviations (RSDs) of 1.06% for the maximum width and 0.46% for depth in terms of the separation channel. The PDMS devices fabricated from the PMMA replica masters were characterized and applied to the separation of a fluorescein isothiocyanate (FITC)-labeled epinephrine sample.

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Immobilization of DNA Hydrogel Plugs in Microfluidic Channels

Cited by 102 publications

References 30 publications

New advances in microchip fabrication for electrochromatography

New advances in microchip fabrication for electrochromatography

A microfluidic DNA computing processor for gene expression analysis and gene drug synthesis

A polymeric master replication technology for mass fabrication of poly(dimethylsiloxane) microfluidic devices

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