Immobilization of commercial laccase onto green coconut fiber by adsorption and its application for reactive textile dyes degradation

Effective removal of sulfur dyes from water by biosorption and subsequent immobilized laccase degradation on crosslinked chitosan beads

“…Before measuring the free laccase activity assay, 0.1 mL of the activated enzyme solution was mixed with 1.9 mL of the ABTS solution (0.4 mM) in an acetate buffer (0.2 M, pH 5.0) at 40 o C [25]. After 30 s, the absorbance at 420 nm was recorded and the linearized kinetic curve was plotted.…”

Immobilization of laccase in a sponge-like hydrogel for enhanced durability in enzymatic degradation of dye pollutants

“…The experiments were conducted in triplicate. The thermal stability parameters were calculated according to the simplified deactivation model as follows [26].…”

“…Environmental stability is one of the most important features concerning the practical application of a biocatalyst [26]. Thus, effects of solution pH and thermal treatment on the residual activity of both free laccase and the Lac-PAM-CTS were carried out to elucidate their stability towards the change of environmental conditions.…”

Laccase, an Emerging Tool to Fabricate Green Composites: A Review

“…Currently, laccase is considered one of the most inexpensive and widely available enzymes used in commercial applications (Brijwani et al 2010;Cristóvão et al 2011). Some of these applications are already in practice, such as pulp processing (Virk et al 2012), detoxification of environmental pollutants (Harms et al 2011), preventing wine browning (Osma et al 2010), oxidation of dyes and their precursors (Kumar et al 2011), and producing lignin from cellulosic material (Wang et al 2014).…”