Immobilization of cellulase on magnetoresponsive graphene nano-supports

Gokhale, Ankush A.; Lu, Jue; Lee, Ilsoon

doi:10.1016/j.molcatb.2013.01.025

Cited by 107 publications

(52 citation statements)

References 90 publications

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“…More specifically, the highest immobilization efficiency was observed for bgl on iron-based nanomaterials, rather than on functionalized GO. Similar immobilization efficiencies have been previously reported for bgl on magnetic nanoparticles (Ricco et al, 2014) or GO-iron nanoparticles hybrid nanomaterials (Gokhale et al, 2013;Ricco et al, 2014). It is interesting to note that the type of amine groups on the surface of the hybrid nanomaterial seems to affect the immobilization efficiency.…”

Section: Immobilization Yield and Activity Of Immobilized Bglsupporting

confidence: 83%

“…The functionalization of magnetic nanoparticles with carbon-based nanomaterials has recently attracted great interest as the resulting hybrid nanomaterials combine the properties of both building blocks. These hybrids can be applied in enzyme immobilization (Jiang et al, 2012;Gokhale et al, 2013) as well as in drug delivery (Yang et al, 2009;Mahmoudi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties

et al. 2018

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Hybrid nanostructures of magnetic iron nanoparticles and graphene oxide were synthesized and used as nanosupports for the covalent immobilization of β-glucosidase. This study revealed that the immobilization efficiency depends on the structure and the surface chemistry of nanostructures employed. The hybrid nanostructure-based biocatalysts formed exhibited a two to four-fold higher thermostability as compared to the free enzyme, as well as an enhanced performance at higher temperatures (up to 70 • C) and in a wider pH range. Moreover, these biocatalysts retained a significant part of their bioactivity (up to 40%) after 12 repeated reaction cycles.

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Section: Immobilization Yield and Activity Of Immobilized Bglsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties

et al. 2018

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“…It can be easily observed that in case of graphene the 2D peak is much narrower and its position is down-shifted. Another difference is observed in the Raman spectrum of graphite in which the 2D band is formed from two elements, namely 2D 1 and 2D 2 [45], which are roughly ¼ and ½ of the height of the G peak, respectively. Graphene exhibits a single sharp 2D signal, approximately four times more intense than G band [47].…”

Section: Graphene and Graphene Oxidementioning

confidence: 96%

“…In addition, once functionalized with biomolecules like polysaccharides [41], proteins [42], etc. or other biological systems graphene can be integrated for developing new applications in biomedicine and bio-nanotechnology such as biosensors [43], biocatalysis [44], biofuel cells [45], etc.…”

Section: Graphene and Graphene Oxidementioning

confidence: 99%

Graphene Nanocomposites Studied by Raman Spectroscopy

Bîru¹,

Iovu²

2018

Raman Spectroscopy

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The goal of this chapter is to provide a general introduction about graphene nanocomposites studied by Raman spectroscopy. The chapter will therefore begin with a brief description of the major Raman bands of carbon allotropes. In the following chapter a concise comparison between single walled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs), fullerenes and graphene is exposed. The characteristic features in Raman spectra of carbon allotropes, namely the intense signals D and G are investigated. In particular, the chapter will outline the Raman spectrum of graphene and different types of graphene oxide. The last part of the chapter is devoted to graphene nanocomposites.

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“…[1][2][3] From the past few decades, nanosized iron oxide particles have attracted great attention in the fields including, but not limited to, immunoassay, 4,5 biosensor, 6,7 bioseparation, 8,9 targeted drug delivery, 10,11 and environmental analysis 12,13 and protein immobilization. [14][15][16][17][18][19][20][21] However, the bare Fe 3 O 4 NPs have high reactivity and easily undergo degradation upon direct exposing to certain environment, leading to poor stability and dispersity. Therefore, the surface of magnetic nanoparticles should be modified to improve the dispersity and biocompatibility, which could significantly facilitate its utilization.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Magnetic Nanoparticles and Its Application in Lipase Immobilization

Xu¹,

Ju²,

Sheng³

et al. 2013

Bulletin of the Korean Chemical Society

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We demonstrate herein the synthesis and modification of magnetic nanoparticles and its use in the immobilization of the lipase. Magnetic Fe 3 O 4 nanoparticles (MNPs) were prepared by simple co-precipitation method in aqueous medium and then subsequently modified with tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethylenesilane (APTES). Silanization magnetic nanoparticles (SMNP) and amino magnetic nanomicrosphere (AMNP) were synthesized successfully. The morphology, structure, magnetic property and chemical composition of the synthetic MNP and its derivatives were characterized using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) analysis, X-ray diffraction, superconducting quantum interference device (SQUID) and thermogravimetric analyses (TGA). All of these three nanoparticles exhibited good crystallization performance, apparent superparamagnetism, and the saturation magnetization of MNP, SMNP, AMNP were 47.9 emu/g, 33.0 emu/g and 19.5 emu/g, respectively. The amino content was 5.66%. The AMNP was used to immobilize lipase, and the maximum adsorption capacity of the protein was 26.3 mg/g. The maximum maintained activity (88 percent) was achieved while the amount of immobilized lipase was 23.7 mg g −1. Immobilization of enzyme on the magnetic nanoparticles can facilitate the isolation of reaction products from reaction mixture and thus lowers the cost of enzyme application.

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Immobilization of cellulase on magnetoresponsive graphene nano-supports

Cited by 107 publications

References 90 publications

Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties

Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties

Graphene Nanocomposites Studied by Raman Spectroscopy

Synthesis and Characterization of Magnetic Nanoparticles and Its Application in Lipase Immobilization

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