Immobilization of carbonic anhydrase on chitosan beads for enhanced carbonation reaction

Wanjari, Snehal; Prabhu, C. N.; Yadav, Reena; Satyanarayana, T.; Labhsetwar, Nitin; Rayalu, Sadhana

doi:10.1016/j.procbio.2011.01.023

Cited by 78 publications

(62 citation statements)

References 24 publications

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“…Immobilized human CA on SBA-15 support 18 also exhibited a high level of CO2 hydration efficiency, in agreement with our current work. The comparison of the amount of CaCO3 precipitated by per microgram CA indicated our results were comparable or higher than literature values 18,36,41 .…”

Section: Co2 Hydration and Conversion To Calcium Carbonatesupporting

confidence: 84%

Preparation of titania based biocatalytic nanoparticles and membranes for CO₂conversion

et al. 2015

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Biomimetic route of CO2 conversion catalyzed by carbonic anhydrase (CA) is an attractive option for carbon capture and storage due to the high efficiency and specificity of CA in CO2 hydration. Preparation of TiO2 based biocatalytic naonparticles and membranes via CA immobilization facilitates the reuse of the enzyme and could be potentially integrated in a gasliquid membrane contactor for high efficient CO2 capture. In this work, different immobilization protocols were compared based on CA loading, activity and stability. For biocatalytic nanoparticles, over 80% activity recovery and 163 mg/g support was achieved. Repeated reuse and recovery of the biocatalytic nanoparticles over twenty cycles showed only modest loss in activity. For the biocatalytic membranes, the nanostructure of the titania coating and the pH values during immobilization were examined to optimize the biocatalytic performance. Biocatalytic membranes prepared at pH 6 with two cycles of sol-gel coating were able to immobilize 700 µg CA/cm 2 nominal membrane area. The CO2 hydration efficiency of the biocatalytic nanoparticles and membranes were examined, and only marginal loss of catalytic efficiency was observed when compared with their free CA counterpart, indicating good potential to apply such biocatalytic nanoparticles and membranes for CO2 conversion.

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Section: Co2 Hydration and Conversion To Calcium Carbonatesupporting

confidence: 84%

Preparation of titania based biocatalytic nanoparticles and membranes for CO₂conversion

et al. 2015

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“…Initial successful attempts of CO 2 sequestration by the aid of carbonic anhydrases were made by bovine carbonic anhydrases, which encouraged investigations from bacterial sources [86,108,116]. Microbially induced carbonate precipitation via carbonic anhydrase has offered the possibility of capturing CO 2 into safe, stable and environmentally benign carbonate reservoirs and a permanent method for disposing off CO 2 [93,94].…”

Section: Co 2 Sequestrationmentioning

confidence: 99%

A review of microbial precipitation for sustainable construction

Achal

Mukherjee

2015

Construction and Building Materials

243

111

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“…The major challenges in enzyme application are limited stability and the difficulties in recovery and reuse (12,13). The immobilization of the enzyme onto appropriate support materials is an attractive way to address those problems.…”

Section: Discussionmentioning

confidence: 99%

“…However, several practical problems such as high cost, limited stability, narrow pH range, and difficulties in recovery and reuse should be solved (13,14). One of the most attractive methods to address these problems is immobilization of enzyme onto porous silica supports (15).…”

mentioning

confidence: 99%

Comparison of amino and epoxy functionalized SBA-15 used for carbonic anhydrase immobilization

Fei

Chen

Dai

et al. 2016

Journal of Bioscience and Bioengineering

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Immobilization of carbonic anhydrase on chitosan beads for enhanced carbonation reaction

Cited by 78 publications

References 24 publications

Preparation of titania based biocatalytic nanoparticles and membranes for CO₂conversion

Preparation of titania based biocatalytic nanoparticles and membranes for CO₂conversion

A review of microbial precipitation for sustainable construction

Comparison of amino and epoxy functionalized SBA-15 used for carbonic anhydrase immobilization

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Immobilization of carbonic anhydrase on chitosan beads for enhanced carbonation reaction

Cited by 78 publications

References 24 publications

Preparation of titania based biocatalytic nanoparticles and membranes for CO2conversion

Preparation of titania based biocatalytic nanoparticles and membranes for CO2conversion

A review of microbial precipitation for sustainable construction

Comparison of amino and epoxy functionalized SBA-15 used for carbonic anhydrase immobilization

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Product

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Preparation of titania based biocatalytic nanoparticles and membranes for CO₂conversion

Preparation of titania based biocatalytic nanoparticles and membranes for CO₂conversion