Immobilization of Candida rugosa Lipase on MCM-41 for the Transesterification of Cotton Seed Oil

Abstract

pure lipase. In literature a variety of carriers, e.g., acrylic resin, textile membrane, polypropylene, celite, mesoporous silica, and diatomaceous earth, have been employed for the lipase immobilization by physical adsorption technique 18). Silica-based porous material, because of their high surface area and tunable pore diameter, also gained popularity in recent past 23) as support for the immobilization of large molecules viz., enzymes. In continuation to our earlier efforts 10, 11, 22) , in present study Candida rugosa lipase (CRL) has been immobilized on MCM-41 by physical adsorption method, and resulted solid biocatalyst (CRL-MCM-41) has been employed for the transesterification of cotton seed oil with methanol. 2 Materials and methods 2.1 Enzyme solutions and instrumentations The solutions of CRL (Sigma-Aldrich) of 3.4 mg/ml concentration were prepared by shaking 68 mg of enzyme in 20 mL buffer solution (25 mM) of desired pH (3-10) at 25℃. Acetate, phosphate and tris/HCl were used for making the buffer solutions of pH 3-5, 6-8, and 9-10, respectively. The CRL solutions thus obtained were centrifuged and clear supernatant was collected and stored at 4℃ for further use. Powder X-ray diffraction (XRD) data has been collected on Panalytical' s X' Pert Pro with Cu Kα radiation, scanning electron microscopy (SEM) images were recorded on JEOL

Burkholderia cepacia lipase: A versatile catalyst in synthesis reactions

“…Lipases are naturally designed to act at an oil‐water interface, which makes them very compatible with organic solvents (Gotor‐Fernández, Brieva, & Gotor, ; Hari Krishna & Karanth, ; Hasan, Shah, & Hameed, ; Jaeger & Eggert, ; Madeira Lau, Van Rantwijk, Seddon, & Sheldon, ; Reetz, ). These enzymes may act in different reaction media, recognize a wide variety of substrates and catalyze a large number of reactions, such as hydrolysis (Charusheela & Arvind, ; Fernandez‐Lorente et al, ; Liu et al, ; Vaysse, Ly, Moulin, & Dubreucq, ), esterifications (Gandhi et al, ; Kontogianni, Skouridou, Sereti, Stamatis, & Kolisis, ; Vaysse et al, ; Zaidi et al, ), alcoholysis (Deng, Xu, Haraldsson, Tan, & Wang, ; Shimada, Watanabe, Sugihara, & Tominaga, ; Soumanou & Bornscheuer, ; Vaysse et al, ), ammoniolysis (De Zoete, Kock‐van Dalen, Van Rantwijk, & Sheldon, ; Gotor‐Fernandez & Gotor, ; Levinson, Kuo, & Kurtzman, ; López‐Serrano, Wegman, van Rantwijk, & Sheldon, ), aminolysis (Badjic, Kadnikova, & Kostic, ; Gotor‐Fernandez & Gotor, ; Torre, Gotor‐Fernández, Alfonso, García‐Alles, & Gotor, ), transesterification (Katiyar & Ali, , ), interesterification (Abigor et al, ; Yang, Fruekilde, & Xu, ; Zhang et al, ) and others.…”

Effect of Metal Ions on the Hydrolytic and Transesterification Activities of Candida rugosa Lipase

“…3.1.1.3 , for the transesterification of triglycerides. Direct application of lipase producing microorganisms as whole cell , pure lipase and immobilized lipase has been well documented in literature for the transesterification of a variety of triglycerides 6,7 . However, the biodiesel production by enzymatic method has not been adopted industrially as enzyme catalyzed reactions are relatively slow 8 and often not lead to the completion of the reaction 96.5 FAMEs yield .…”

Chitosan/Polyvinylpyrrolidone/MCM‐41 Composite Hydrogel Films: Structural, Thermal, Surface, and Antibacterial Properties

“…The band at 810 cm −1 was associated to the symmetric stretching vibration of the rocking mode of SiOSi . In addition, pure MCM‐41 shows an intense band at 1080 cm −1 due to SiO asymmetric stretching vibrations . Other absorption bands found at 440 cm −1 and 3400 cm −1 , which attributed to SiOSi bending of surface silanols and adsorbed water molecules, respectively .…”