Immobilization of Candida antarctica Lipase B by Covalent Attachment to Green Coconut Fiber

Brígida, Ana Iraidy S.; Pinheiro, Álvaro Daniel Teles; Ferreira, Andrea Lopes de Oliveira; Pinto, Gustavo Adolfo Saavedra; Gonçalves, Luciana Rocha Barros

doi:10.1007/978-1-60327-181-3_7

Cited by 17 publications

(11 citation statements)

References 24 publications

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“…Preparation of Immobilized Enzyme Immobilized enzyme was obtained by adsorption, at room temperature, by contacting enzyme solution and support in 5 ml syringes, stirred using the apparatus presented in a previous work [27]. For each gram of dry support, 10 ml of lipase solution in 25 mM sodium phosphate buffer was used.…”

Section: Protein Determinationmentioning

confidence: 99%

“…2 ) and making A equal to 50%. In this work, stabilization factor (F) was considered as the ratio of immobilized enzymes' half-lives to soluble enzyme half-life [27].…”

Section: Protein Determinationmentioning

confidence: 99%

“…However, although more molecules were immobilized on coconut fiber, not all of them were available to the substrate, causing a decrease in recovered activity. Coconut fiber surface does not have a porous structure, and it has a low surface area [27]. This poor surface area limits the number of enzyme molecules to be immobilized, facilitating the multilayer adsorption of proteins.…”

Section: Influence Of Contact Time and Enzyme Concentration On The Immentioning

confidence: 99%

“…These studies showed that agroindustrial wastes are a suitable raw material source for immobilization matrixes. Previous studies [27] had shown that green coconut fiber is a suitable support for lipase immobilization by covalent attachment, as an increase in thermal and operational stabilities were obtained when compared with the soluble enzyme.…”

Section: Introductionmentioning

confidence: 99%

“…Low-cost supports can be organic (chitin, chitosan) or inorganic (CaCO 3 ) [21]. From organic group, it has been detached from lignocellulosic agroindustrial waste supports, such as saw dust, straw, wood chips/shavings, rice husk [22], spent grains [23], rice straw [21,24], cellulignin [25], and coconut fiber [26,27]. These studies showed that agroindustrial wastes are a suitable raw material source for immobilization matrixes.…”

Section: Introductionmentioning

confidence: 99%

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Immobilization of Candida antarctica Lipase B by Adsorption to Green Coconut Fiber

Brígida

Pinheiro

Ferreira

et al. 2007

Appl Biochem Biotechnol

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An agroindustrial residue, green coconut fiber, was evaluated as support for immobilization of Candida antarctica type B (CALB) lipase by physical adsorption. The influence of several parameters, such as contact time, amount of enzyme offered to immobilization, and pH of lipase solution was analyzed to select a suitable immobilization protocol. Kinetic constants of soluble and immobilized lipases were assayed. Thermal and operational stability of the immobilized enzyme, obtained after 2 h of contact between coconut fiber and enzyme solution, containing 40 U/ml in 25 mM sodium phosphate buffer pH 7, were determined. CALB immobilization by adsorption on coconut fiber promoted an increase in thermal stability at 50 and 60 degrees C, as half-lives (t (1/2)) of the immobilized enzyme were, respectively, 2- and 92-fold higher than the ones for soluble enzyme. Furthermore, operational stabilities of methyl butyrate hydrolysis and butyl butyrate synthesis were evaluated. After the third cycle of methyl butyrate hydrolysis, it retained less than 50% of the initial activity, while Novozyme 435 retained more than 70% after the tenth cycle. However, in the synthesis of butyl butyrate, CALB immobilized on coconut fiber showed a good operational stability when compared to Novozyme 435, retaining 80% of its initial activity after the sixth cycle of reaction.

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Section: Protein Determinationmentioning

confidence: 99%

“…2 ) and making A equal to 50%. In this work, stabilization factor (F) was considered as the ratio of immobilized enzymes' half-lives to soluble enzyme half-life [27].…”

Section: Protein Determinationmentioning

confidence: 99%

Section: Influence Of Contact Time and Enzyme Concentration On The Immentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%