Immobilization of Candida antarctica Lipase B by Adsorption to Green Coconut Fiber

Abstract

An agroindustrial residue, green coconut fiber, was evaluated as support for immobilization of Candida antarctica type B (CALB) lipase by physical adsorption. The influence of several parameters, such as contact time, amount of enzyme offered to immobilization, and pH of lipase solution was analyzed to select a suitable immobilization protocol. Kinetic constants of soluble and immobilized lipases were assayed. Thermal and operational stability of the immobilized enzyme, obtained after 2 h of contact between coconut fiber and enzyme solution, containing 40 U/ml in 25 mM sodium phosphate buffer pH 7, were determined. CALB immobilization by adsorption on coconut fiber promoted an increase in thermal stability at 50 and 60 degrees C, as half-lives (t (1/2)) of the immobilized enzyme were, respectively, 2- and 92-fold higher than the ones for soluble enzyme. Furthermore, operational stabilities of methyl butyrate hydrolysis and butyl butyrate synthesis were evaluated. After the third cycle of methyl butyrate hydrolysis, it retained less than 50% of the initial activity, while Novozyme 435 retained more than 70% after the tenth cycle. However, in the synthesis of butyl butyrate, CALB immobilized on coconut fiber showed a good operational stability when compared to Novozyme 435, retaining 80% of its initial activity after the sixth cycle of reaction.

Use of Yarrowia lipolytica Lipase Immobilized in Cell Debris for the Production of Lipolyzed Milk Fat (LMF)

“…A deactivation rate coefficient, k d , of 0.08 h −1 and a half-life (t 1/2 ) of 8.7 h were determined at these conditions. Higher k d and lower t 1/2 were found for immobilized lipase B from C. antarctica obtained by covalent attachment at pH 7.0 [26]. Kumari and Gupta [22] also found lower t 1/2 for a lipase from Trichosporon asahii , which shows that LipImDebri has a good thermal stability at 37 °C.…”

Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles

“…[15][16][17][18] For example, work has addressed support surface hydrophobicity/hydrophobocity [19,20,21] and 3 enzyme solution pH. [17,22,23] These parameters have large influence on the total amount of enzyme loading and enzyme-catalyst activity. [24,25] Hydrophobic binding of lipases by adsorption has proved successful due to its affinity for water/oil interfaces.…”

Successive cycles of utilization of novozym 435 in three different reaction systems

“…After 4 cycles, no significant difference was observed in the production of isoamyl acetate at 37 o C. After 10 cycles, a reduction of about 50% in the conversion was observed and the residual activity was 76%. Brígida et al (2008) evaluated the use of coconut fiber residue as a support for immobilization of lipase from Candida antarctica type B (CALB). The authors studied the cycles of reuse of the immobilized enzyme and compared the results with those obtained for Novozym 435.…”