Immobilization of bacteriophage Qβ on metal-derivatized surfaces via polyvalent display of hexahistidine tags

Udit, Andrew K.; Brown, Steven D.; Baksh, Michael M.; Finn, M. G.

doi:10.1016/j.jinorgbio.2008.08.003

Cited by 24 publications

(25 citation statements)

References 27 publications

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“…The filamentous bacteriophage M13 is most commonly employed for phage display, and M13 systems are at the forefront of research in nanomaterials [3][4][5][6][7] and nanomedicine [8][9][10][11]. Fusion proteins have been displayed on every one of the five M13 coat proteins [12][13][14][15], and platforms utilizing combinations of several, but not all, coat proteins have been developed [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Phage display selection of tight specific binding variants from a hyperthermostable Sso7d scaffold protein library

2016

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Antibodies, the quintessential biological recognition molecules, are not ideal for many applications because of their large size, complex modifications, and thermal and chemical instability. Identifying alternative scaffolds that may be evolved into tight, specific binding molecules with improved physical properties is of increasing interest, particularly for biomedical applications in resource-limited environments. Hyperthermophilic organisms, such as Sulfolobus solfataricus, are an attractive source of highly stable proteins that may serve as starting points for alternative molecular recognition scaffolds. We describe the first application of phage display to identify binding proteins based on the S. solfataricus protein Sso7d scaffold. Sso7d is a small cysteine-free DNA-binding protein (approximately 7 kDa, 63 amino acids), with a melting temperature of nearly 100°C. Tight-binding Sso7d variants were selected for a diverse set of protein targets from a 10 10 member library, demonstrating the versatility of the scaffold. These Sso7d variants are able to discriminate among closely related human, bovine and rabbit serum albumins. Equilibrium dissociation constants in the nanomolar to low micromolar range were measured via competitive ELISA. Importantly, the Sso7d variants continue to bind their targets in the absence of the phage context. Furthermore, phage-displayed Sso7d variants retain their binding affinity after exposure to temperatures up to 70°C. Taken together, our results suggest that the Sso7d scaffold will be a complementary addition to the range of non-antibody scaffold proteins that may be utilized in phage display. Variants of hyperthermostable binding proteins have potential applications in diagnostics and therapeutics for environments with extreme conditions of storage and deployment.

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Section: Introductionmentioning

confidence: 99%

Phage display selection of tight specific binding variants from a hyperthermostable Sso7d scaffold protein library

2016

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“…For example, genetic engineering can be applied to introduce novel amino acids to serve as targets for bioconjugation, 19,20 to introduce affinity tags, 21–23 or to alter the surface charge. 24 …”

Section: Introductionmentioning

confidence: 99%

Engineering of Brome mosaic virus for biomedical applications

et al. 2012

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Viral nanoparticles (VNPs) are becoming versatile tools in platform technology development. Their well-defined structures as well as their programmability through chemical and genetic modification allow VNPs to be engineered for potential imaging and therapeutic applications. In this article, we report the application of a variety of bioconjugation chemistries to the plant VNP Brome mosaic virus (BMV). Functional BMV nanoparticles displaying multiple copies of fluorescent dyes, PEG molecules, chemotherapeutic drug moieties, targeting proteins and cell penetrating peptides were formulated. This opens the door for the application of BMV in nanomedicine.

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“…Genetic engineering can change the basic structure of the viral CP by the insertion, removal, or substitution of particular amino acid residues. [ 68 ] This can include the addition or excision of sequences from the N‐ or C‐terminus of the CP, the insertion of targeting sequences to surface loops, or the substitution of individual amino acids to introduce functional groups. Cysteine mutants are the most popular of these constructs because they potentially form disulfide linkages, allowing their association with gold particles and their bioconjugation with thiol‐selective chemistries.…”

Section: Strategies For Multifunctional Vlp Formulationsmentioning

confidence: 99%

Virus‐Like Particles as Theranostic Platforms

Sun

Cui

2020

Advanced Therapeutics

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In recent years, theranostics have received enormous attention for their use in individualized diagnosis and treatment. The ability of viral coat protein subunits to assemble hierarchically can be used to package various kinds of foreign compounds. Moreover, numerous chemistries and modification strategies allow the functionalization of these virus-like particles (VLPs) with imaging reagents, targeting ligands, or therapeutic molecules. VLP nanoplatforms have great potential utility in the design of sophisticated multifunctional theranostic platforms. Numerous studies have reported the construction of multifunctional nanoparticles using VLPs for targeted diagnoses and treatment. In this paper, the latest progress in molecular imaging, drug delivery, and multifunctional theranostic nanodevices based on VLPs is reviewed.

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Immobilization of bacteriophage Qβ on metal-derivatized surfaces via polyvalent display of hexahistidine tags

Cited by 24 publications

References 27 publications

Phage display selection of tight specific binding variants from a hyperthermostable Sso7d scaffold protein library

Phage display selection of tight specific binding variants from a hyperthermostable Sso7d scaffold protein library

Engineering of Brome mosaic virus for biomedical applications

Virus‐Like Particles as Theranostic Platforms

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