Immobilization of Aspergillus japonicus by entrapping cells in gluten for production of fructooligosaccharides

Chien, Ching-Shan; Lee, Wen‐Chien; Lin, Tsao-Jen

doi:10.1016/s0141-0229(01)00384-2

Cited by 72 publications

(48 citation statements)

References 21 publications

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“…The 6-M69 and 4-M37 mutants have been shown to have higher FTase activity than the parental strains and this demonstrates the potential of these strains for use in cell immobilization techniques for the production of FOS (Chien et al, 2001). This increase of about 1.5 fold in FTase activity can be compared to the results obtained by Guilarte et al (2009) The FTase activity and, consequently, the production of FOS increased dramatically by performing the reaction in 2 mL tubes in a ThermoMixer®.…”

Section: Discussionmentioning

confidence: 90%

Obtaining mutant fungal strains of Aspergillus niger with high production of fructooligosaccharides (FOS) using ultraviolet light irradiation

Castillo¹,

DÃez-Municio²,

Cruz³

et al. 2017

Afr. J. Biotechnol.

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A strain of Aspergillus niger PR-142 native to northern coast of Peru was subjected to successive processes of mutagenesis by ultraviolet light (UV) irradiation at 253.7 nm to increase the production of fructooligosaccharides (FOS). An initial selection was made by considering the mutants with increased invertase activity followed by the measurement of β-fructosyltransferase (FTase) activity both in mycelium and extracellular environment. Five selected mutants, which showed increased values of mycelium invertase activity (ranging from 101 to 128% as compared to the parent strain) at 40°C and sodium dodecylsulfate 0.15 (w/v), were grown in a fermentative medium in 50 mL conical tubes on a rotary shaker, and their FTase activity was determined. The 6-M69 mutant showed the most active mycelium activity of 1.5 fold as compared to the parent strain. When the same reaction was performed between 1 to 4 h, at the 3 rd h, the mycelium FTase activity significantly increased up to 7 and 3 times in the mutant and parental strain, respectively. Finally, 4 mutants and the parental PR-142 were genetically characterized using inter simple sequence repeat polymerase chain reaction (ISSR-PCR) molecular markers. This analysis showed a significant 33% polymorphic bands between the parent and mutant markers, and 20 bands were unique to the mutants.

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Section: Discussionmentioning

confidence: 90%

Obtaining mutant fungal strains of Aspergillus niger with high production of fructooligosaccharides (FOS) using ultraviolet light irradiation

Castillo¹,

DÃez-Municio²,

Cruz³

et al. 2017

Afr. J. Biotechnol.

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“…However, the interest to produce FOS using different fungus such as Aspergillus, Aureobasidum, and Penicillium, in which these enzymes have been found, has increased in the last years. Aspergillus japonicus has been considered a potential strain for industrial production of FOS by SSF (Chien et al, 2001;Mussatto et al, 2009), and the development of a viable and economic process that permits to obtain high volumetric productivity is one of the main challenges to be overcome.…”

Section: Fructooligosaccharides Productionmentioning

confidence: 99%

Use of Agro-Industrial Wastes in Solid-State Fermentation Processes

Mussatto¹,

Ballesteros²,

Martins³

et al. 2012

Industrial Waste

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“…Classical mutagenesis with physical and/or chemical agents followed by titre test of a large number of isolates has been used successfully to improve the productivity of several fungal metabolites and enzymes (4,19,22). α-Amylase overproducing mutant of Aspergillus oryzae, which was obtained by random mutagenesis showed 6.73 times more dextrinizing and 5.13 times more saccharogenic activities than the parent strain (3).…”

Section: Production Of -Fructofuranosidase By a Oryzaementioning

confidence: 99%

“…Fructooligosaccharides (FOS) are produced from sucrose by the action of enzymes with transfructosylating activity, which have been classified as β-D-fructofuranosidases (EC 3.2.1.26) or fructosyltransferases (EC 2.4.1.9) (1,6). Although several microorganisms such as Fusarium sp.…”

Section: Introductionmentioning

confidence: 99%

Mutagenesis of Aspergillus oryzae IPT-301 to improve the production of β-fructofuranosidase

Maresma¹,

Castillo²,

Fernandez³

et al. 2010

Braz. J. Microbiol.

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Aspergillus oryzae IPT-301, previously reported as a β-fructofuranosidase producing microorganism, was successfully mutated using UV irradiation at 253.7 nm followed by the screening of survivors resistant to certain stress conditions. Strains were first subjected to the β-fructofuranosidase activity assay using a portion from the colony grown in Petri dish as the enzyme source. Seven mutants with β-fructofuranosidase activity values relative to the parent culture between 140 -190% were selected from survivors grown at temperature of 40ºC or 0.018% (w/v) sodium dodecyl sulfate concentration. They were cultivated on a rotary shaker to characterize mycelium and extracellular fructosyltransferase activities.Three mutants named IPT-745, IPT-746 and IPT-748 showed the highest amount of mycelium activity whose values increased 1.5 -1.8 fold, compared with the parent strain. It was found that more than 55% of total enzyme activity (mycelium-plus extracellular-activity) from these strains was detected in the mycelium fraction. Only one mutant, IPT-747, exceeded the amount of extracellular enzyme exhibited by the parent strain (1.5 times). This mutant also showed the highest value of total fructosyltransferase activity.

show abstract

Immobilization of Aspergillus japonicus by entrapping cells in gluten for production of fructooligosaccharides

Cited by 72 publications

References 21 publications

Obtaining mutant fungal strains of Aspergillus niger with high production of fructooligosaccharides (FOS) using ultraviolet light irradiation

Obtaining mutant fungal strains of Aspergillus niger with high production of fructooligosaccharides (FOS) using ultraviolet light irradiation

Use of Agro-Industrial Wastes in Solid-State Fermentation Processes

Mutagenesis of Aspergillus oryzae IPT-301 to improve the production of β-fructofuranosidase

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